Overexpression of tau in a nonneuronal cell induces long cellular processes.
Open accessAbstract
The ways in which the various microtubule-associated proteins (MAPs) contribute to cellular function are unknown beyond the ability of these proteins to modify microtubule dynamics. One member of the MAP family, tau protein, is restricted in its distribution to the axonal compartment of neurons, and has therefore prompted studies that attempt to relate tau function to the generation or maintenance of this structure. Sf9 cells from a moth ovary, when infected with a baculovirus containing a tau cDNA insert, elaborate very long processes. This single gene product expressed in a foreign host cell grossly alters the normal rounded morphology of these cells. The slender, relatively nonbranched appearance of these processes as well as their uniform caliber resembles the light-microscopic appearance of axons observed in several neuronal culture systems. Immunolabeling of the tau-expressing Sf9 cells demonstrated tau reactivity in the induced processes, and EM that microtubule bundles were present in the processes. Microtubule stabilization alone was insufficient to generate processes, since taxol treatment did not alter the overall cell shape, despite the induction of microtubule bundling within the cell body.
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The interaction of Tau with actin is important for neurite outgrowth and synaptic dysfunction.
“The interaction of Tau with actin is important for neurite outgrowth and synaptic dysfunction11, 12, 22.”
From Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau (2017) · cites this paper as [11]
The Introduction (p. 725) links tau to neurite elongation, and the discussion reports that tau-expressing Sf9 cells developed long processes. The paper does not establish that tau’s interaction with actin drives outgrowth or synaptic dysfunction; it says synaptic vesicles were not observed in those processes. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe interaction of MAPs with actin is important for neurite outgrowth.
“The interaction of MAPs with actin is important for neurite outgrowth11, 12.”
From Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau (2017) · cites this paper as [11]
The paper reports that tau expression induces long processes in nonneuronal Sf9 cells and, separately, that cytochalasin treatment produces short retraction processes by disrupting the actin network. The passages on process formation and cytochalasin do not address whether MAPs interact with actin or whether such an interaction is important for neurite outgrowth (searched: actin interaction, tau actin cytoskeleton). (LLM, conf=0.72)
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Cites
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Glycated tau protein in Alzheimer disease: a mechanism for induction of oxidant stress.
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- Authors
- Knops, J., Kosik, K. S., Lee, G., Pardee, J. D., Cohen-Gould, L., & McConlogue, L.
- Affiliations
- Athena Neuroscience Inc., San Francisco, California.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1991-08-15
- Volume
- 114
- Issue
- 4
- Pages
- 725-733
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2289897
- OpenAlex
- W2051358675
- Cited by
- 286
- References
- 3
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 7 · 2019: 2 · 2018: 7 · 2017: 6 · 2016: 3 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 18.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG 06601, AG06172, R01AG006172, R01AG006601)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- tau protein, microtubule-associated proteins, non-neuronal cells, Sf9 cells, baculovirus expression system, cell morphology, microtubule stabilization, paclitaxel, microtubule bundling, axon formation
- MeSH terms
- Alkaloids, Animals, Antineoplastic Agents, Phytogenic, Cell Line, Genetic Vectors, Microscopy, Electron, Microtubule-Associated Proteins, Microtubules, Moths, Nerve Tissue Proteins, Transfection, Cytochalasin D, Polymerase Chain Reaction, Baculoviridae, tau Proteins, Paclitaxel
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