MAP2 and tau segregate into dendritic and axonal domains after the elaboration of morphologically distinct neurites: an immunocytochemical study of cultured rat cerebrum

Kosik, KS; Finch, EA

doi:10.1523/jneurosci.07-10-03142.1987

Cited by 368Alzheimer's disease research and treatmentsNeuroscience and Neuropharmacology ResearchAxon Guidance and Neuronal Signaling

MAP2 and tau segregate into dendritic and axonal domains after the elaboration of morphologically distinct neurites: an immunocytochemical study of cultured rat cerebrum

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Kosik, KS, Finch, EA·1987·The Journal of Neuroscience

DOI 10.1523/jneurosci.07-10-03142.1987PMC PMC6569170

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Abstract

We sought to determine whether the strict segregation of MAP2 and tau into somatodendritic and axonal compartments in situ was maintained in dissociated neuronal cultures of the rat cerebrum. Cultures grown under serum-free conditions were immunolabeled with monoclonal antibodies specific for MAP2 and tau. At 14 d after plating, a clear distinction between MAP2- and tau-immunoreactive neurites was apparent. MAP2- immunoreactive neurites were relatively short, thick, tapering, and branched. Tau-immunoreactive neurites formed a crisscrossing meshwork of long, fine-caliber neurites, which, in more densely plated cultures, had a tendency to form thick, ropelike fascicles. Unlike the MAP2 pattern, tau antibodies labeled somata only lightly. Since distinct populations of neurites were labeled with the 2 antibodies, we sought to observe the development of the topographically distinct compartments by double-labeled immunocytochemistry with both polyclonal and monoclonal antibodies to MAP2 and tau. Cells observed within the first 8 hr after plating demonstrated equally intense MAP2 and tau immunoreactivity in a coextensive distribution throughout the cell body and initial neurites. By 16 hr, some neurites began to assume dendritic and axonal features; however, many such processes contained reaction product for both MAP2 and tau. Beginning at this time, neurites that appeared axonal showed a progressively weaker reaction with MAP2 antibodies, and neurites that appeared dendritic showed a progressively weaker reaction with tau antibodies. In most neurites the diminution appeared to occur uniformly over the entire extent of the neurite. During this transformation period there were occasional axon-like neurites that contained MAP2 immunoreactivity proximally, while tau immunoreactivity extended over the entire length of the neurite. We conclude that neurons in culture are able to compartmentalize MAP2 and tau into their appropriate processes and only attain an apparently homogeneous population of one of these MAPs after the neuron has assumed dendritic and axonal features. The analysis also lends indirect support to the hypothesis that microtubule-associated proteins (MAPs) form this association at the distal extent of the growing neurite.

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MAP2 is a marker for mature dendrites.
“contained MAP2 (Supplementary video 4), a marker for mature dendrites38.”
From Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB (2021) · cites this paper as [38]

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Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue
Mccarthy, K., & de Vellis, J. (1980) · Journal of Cell Biology · doi:10.1083/jcb.85.3.890 · Cited by 3,915 · see citing works
PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY
McLean, I. W., & Nakane, P. K. (1974) · Journal of Histochemistry & Cytochemistry · doi:10.1177/22.12.1077 · Cited by 3,817 · see citing works
The distribution of tau in the mammalian central nervous system.
Binder, L. I., Frankfurter, A., & Rebhun, L. I. (1985) · The Journal of cell biology · doi:10.1083/jcb.101.4.1371 · Cited by 1,588 · see citing works
Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside.
Ranscht, B., Clapshaw, P. A., Price, J., Noble, M., & Seifert, W. E. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.8.2709 · Cited by 834 · see citing works
Intracellular localization of messenger RNAs for cytoskeletal proteins
Lawrence, J. B. (1986) · Cell · doi:10.1016/0092-8674(86)90326-0 · Cited by 649 · see citing works
Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture
Caceres, A., Banker, G., & Binder, L. (1986) · The Journal of Neuroscience · doi:10.1523/jneurosci.06-03-00714.1986 · Cited by 367 · cited 2× · see citing works
“The work of Caceres et al. (1986) with rat hippocampal cultures describes the progressive loss of MAP2 immunoreactivity in a proximodistal fashion during in vitro development of an axon.”
Background
High molecular weight microtubule-associated proteins are preferentially associated with dendritic microtubules in brain.
Matus, A. I., Bernhardt, R. R., & Hugh-Jones, T. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.5.3010 · Cited by 362 · see citing works
An electron microscopic study of the development of axons and dendrites by hippocampal neurons in culture. I. Cells which develop without intercellular contacts
Bartlett, W., & Banker, G. (1984) · Journal of Neuroscience · doi:10.1523/jneurosci.04-08-01944.1984 · Cited by 348 · see citing works
An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain.
Shaw, G., Osborn, M., & Weber, K. (1981) · PubMed · Cited by 305 · see citing works
Assembly of microtubules at the tip of growing axons
Bamburg, J. R., Bray, D., & Chapman, K. (1986) · Nature · doi:10.1038/321788a0 · Cited by 279 · see citing works
Microtubule-associated protein 2: monoclonal antibodies demonstrate the selective incorporation of certain epitopes into Alzheimer neurofibrillary tangles.
Kosik, K. S., Duffy, L. K., Dowling, M. M., Abraham, C. R., McCluskey, A. H., & Selkoe, D. J. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.24.7941 · Cited by 223 · see citing works
Self-citation
An electron microscopic study of the development of axons and dendrites by hippocampal neurons in culture. II. Synaptic relationships
Bartlett, W., & Banker, G. (1984) · The Journal of Neuroscience · doi:10.1523/jneurosci.04-08-01954.1984 · Cited by 222 · see citing works
Studies on the expression of the microtubule-associated protein, tau, during mouse brain development, with newly isolated complementary DNA probes.
Drubin, D. G., Caput, D., & Kirschner, M. W. (1984) · The Journal of Cell Biology · doi:10.1083/jcb.98.3.1090 · Cited by 164 · see citing works
Changes in composition and activity of microtubule-associated proteins during brain development
Mareck, A., Fellous, A., Francon, J., & Nunez, J. (1980) · Nature · doi:10.1038/284353a0 · Cited by 160 · see citing works
Heterogeneity of Microtubule‐Associated Proteins and Brain Development
Francon, J., Lennon, A. M., Fellous, A., Mareck, A., Pierre, M., & Nunez, J. (1982) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1982.tb07072.x · Cited by 155 · see citing works
Morphological classification of rat cortical neurons in cell culture
Kriegstein, A. R., & Dichter, M. (1983) · Journal of Neuroscience · doi:10.1523/jneurosci.03-08-01634.1983 · Cited by 145 · see citing works
Ontogeny of microtubule-associated protein 2 in rat cerebellum: Differential expression of the doublet polypeptides
Burgoyne, R. D., & Cumming, R. (1984) · Neuroscience · doi:10.1016/0306-4522(84)90220-3 · Cited by 143 · see citing works
Hirano bodies contain tau protein
Galloway, P. G., Perry, G. L., Kosik, K. S., & Gambetti, P. L. (1987) · Brain Research · doi:10.1016/0006-8993(87)90071-0 · Cited by 93 · see citing works
Self-citation
Frozen tissue sections as an experimental system to reveal specific binding sites for the regulatory subunit of type II cAMP-dependent protein kinase in neurons.
Miller, P., Walter, U., Theurkauf, W. E., Vallee, R. B., & Camilli, P. V. D. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.18.5562 · Cited by 90 · see citing works
Axonal transport of a subclass of tau proteins: evidence for the regional differentiation of microtubules in neurons.
Tytell, M., Brady, S. T., & Lasek, R. J. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1570 · Cited by 90 · see citing works
Architecture of apical dendrites in the murine neocortex: Dual apical dendritic systems
Escobar, M. I., Pimienta, H. J., Caviness, V. S., Jacobson, M., Crandall, J., & Kosik, K. S. (1986) · Neuroscience · doi:10.1016/0306-4522(86)90074-6 · Cited by 86 · see citing works
Self-citation
Ontogenesis of microtubule-associated protein 2 (MAP2) in embryonic mouse cortex
Crandall, J. E., Jacobson, M., & Kosik, K. S. (1986) · Developmental Brain Research · doi:10.1016/0165-3806(86)90072-6 · Cited by 78 · see citing works
Self-citation
Intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons
Neale, E. A., Macdonald, R. L., & Nelson, P. G. (1978) · Brain Research · doi:10.1016/0006-8993(78)90255-x · Cited by 73 · see citing works
Spine formation and maturation of type 1 synapses on spiny stellate neurons in primate visual cortex
Mates, S. L., & Lund, J. S. (1983) · The Journal of Comparative Neurology · doi:10.1002/cne.902210107 · Cited by 58 · see citing works
Postnatal rat sympathetic neurons in culture. I. A comparison with embryonic neurons
Wakshull, E., Johnson, M. I., & Burton, H. (1979) · Journal of Neurophysiology · doi:10.1152/jn.1979.42.5.1410 · Cited by 53 · see citing works
Expression of Microtubule‐Associated Proteins During the Early Stages of Neurite Extension by Brain Neurons Cultured in a Defined Medium
Couchie, D., Faivre‐Bauman, A., Puymirat, J., Guilleminot, J., Tixier‐Vidal, A., & Nunez, J. (1986) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1986.tb00748.x · Cited by 35 · see citing works
Changes in the cytoplasmic distribution of microtubule-associated protein 2 during the differentiation of cultured cerebellar granule cells
Alaimo-Beuret, D., & Matus, A. I. (1985) · Neuroscience · doi:10.1016/0306-4522(85)90280-5 · Cited by 33 · see citing works
Proteins transported in slow components a and b of axonal transport are distributed differently in the transverse plane of the axon.
Heriot, K., Gambetti, P., & Lasek, R. J. (1985) · The Journal of Cell Biology · doi:10.1083/jcb.100.4.1167 · Cited by 30 · see citing works

Flagged first, then by citations

Research works printed but not linked: 3

Binder, L. I., A. Frankfurter, H. Kim, A. Caceres, M. R. Payne, and L. I. Rebhun (1984) Heterogeneity of microtubule-associated protein 2 during rat brain. Proc. Natl. Acad. Sci. USA. 81: 5613-5617.
Kosik, K. S. C. L. Joachim, and D. J. Selkoe (1986) Microtubule- associated protein tau is a major antigenic component of paired helical filaments in Alzheimer's disease. Proc. Natl. Acad. Sci. USA 83: 4044-4048.
Towbin, H., T. Staehelin, and J. Gordon (1979) Electrophoretictransfer of proteins from polyacrylamide gels to nitrocellulose sheets: Proce- dure and some auolications. Proc. Natl. Acad. Sci. USA 76: 4350- __ 4354.

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Keller, J. N., & Markesbery, W. R. (2000) · Journal of Neuroscience Research · doi:10.1002/1097-4547(20000815)61:4 · Cited by 54 · see citing works
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Buée, L., Bussière, T., Buée‐Scherrer, V., Delacourte, A., & Hof, P. R. (2000) · Brain Research Reviews · doi:10.1016/s0165-0173(00)00019-9 · Cited by 2,007 · see citing works
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Pleasure, S., Page, C., & Lee, V. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-05-01802.1992 · Cited by 607 · see citing works
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Butner, K. A., & Kirschner, M. W. (1991) · The Journal of cell biology · doi:10.1083/jcb.115.3.717 · Cited by 550 · see citing works
The microtubule binding domain of tau protein
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Suppression of MAP2 in cultured cerebeller macroneurons inhibits minor neurite formation
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Kobayashi, S., Ishiguro, K., Omori, A., Takamatsu, M., Arioka, M., Imahori, K., & Uchida, T. (1993) · FEBS Letters · doi:10.1016/0014-5793(93)80723-8 · Cited by 234 · see citing works
Novel diffusion barrier for axonal retention of Tau in neurons and its failure in neurodegeneration
Li, X., Kumar, Y., Zempel, H., Mandelkow, E., Biernat, J., & Mandelkow, E. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.376 · Cited by 218 · see citing works
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Li, Y., Jiang, N., Powers, C., & Chopp, M. (1998) · Stroke · doi:10.1161/01.str.29.9.1972 · Cited by 218 · see citing works
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Yu, W., & Baas, P. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-05-02818.1994 · Cited by 213 · see citing works
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Axonal growth and fasciculation linked to differential expression of BDNF and NT3 receptors in developing cerebellar granule cells
Segal, R. A., Pomeroy, S. L., & Stiles, C. D. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-07-04970.1995 · Cited by 212 · see citing works
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Self-citation
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Ferreira, A., Niclas, J., Vale, R. D., Banker, G. A., & Kosik, K. S. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.117.3.595 · Cited by 190 · see citing works
Self-citation
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Zemlan, F. P., Rosenberg, W. S., Luebbe, P., Campbell, T. A., Dean, G. E., Weiner, N. E., Cohen, J. A., Rudick, R. A., & Woo, D. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0720741.x · Cited by 187 · see citing works
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Okabe, S., & Hirokawa, N. (1990) · Nature · doi:10.1038/343479a0 · Cited by 186 · see citing works
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Jones, S. L., & Svitkina, T. (2016) · Neural Plasticity · doi:10.1155/2016/6808293 · Cited by 184 · see citing works
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Panda, D., Goode, B. L., Feinstein, S. C., & Wilson, L. (1995) · Biochemistry · doi:10.1021/bi00035a017 · Cited by 185 · see citing works
Sense and antisense transfection analysis of tau function: tau influences net microtubule assembly, neurite outgrowth and neuritic stability
Esmaeli‐Azad, B., McCarty, J. H., & Feinstein, S. C. (1994) · Journal of Cell Science · doi:10.1242/jcs.107.4.869 · Cited by 184 · see citing works
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Sudo, H., & Baas, P. W. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.0048-10.2010 · Cited by 181 · see citing works
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Li, C., & Götz, J. (2017) · The EMBO Journal · doi:10.15252/embj.201797724 · Cited by 178 · see citing works
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Khatoon, S., Grundke, I., & Iqbal, K. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)00829-9 · Cited by 167 · see citing works
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Wiche, G., Oberkanins, C., & Himmler, A. (1991) · International review of cytology · doi:10.1016/s0074-7696(08)61528-4 · Cited by 165 · see citing works
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Self-citation
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Sluch, V. M., Davis, C.-H. O., Jaskula-Ranga, V., Kerr, J. M., Krick, K., Martin, R., Berlinicke, C. A., Marsh‐Armstrong, N., Diamond, J. S., Mao, H., et al. (2015) · Scientific Reports · doi:10.1038/srep16595 · Cited by 154 · see citing works
A Primate lncRNA Mediates Notch Signaling during Neuronal Development by Sequestering miRNA
Rani, N., Nowakowski, T. J., Zhou, H., Godshalk, S. E., Lisi, V., Kriegstein, A. R., & Kosik, K. S. (2016) · Neuron · doi:10.1016/j.neuron.2016.05.005 · Cited by 153 · see citing works
Self-citation
Dendritic Ca2+ Channels Characterized by Recordings from Isolated Hippocampal Dendritic Segments
Kavalali, E. T., Zhuo, M., Bito, H., & Tsien, R. W. (1997) · Neuron · doi:10.1016/s0896-6273(00)80305-0 · Cited by 148 · see citing works
Frequent and symmetric deposition of misfolded tau oligomers within presynaptic and postsynaptic terminals in Alzheimer¿s disease
Tai, H., Wang, B. Y., Serrano‐Pozo, A., Frosch, M. P., Spires‐Jones, T. L., & Hyman, B. T. (2014) · Acta Neuropathologica Communications · doi:10.1186/s40478-014-0146-2 · Cited by 142 · see citing works
Sorting mechanisms of Tau and MAP2 in neurons: Suppressed axonal transit of MAP2 and locally regulated microtubule binding
Kanai, Y., & Hirokawa, N. (1995) · Neuron · doi:10.1016/0896-6273(95)90298-8 · Cited by 144 · see citing works
Processes induced by tau expression in Sf9 cells have an axon-like microtubule organization.
Baas, P. W., Pienkowski, T. P., & Kosik, K. S. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.115.5.1333 · Cited by 143 · see citing works
Self-citation
Distribution of the phosphorylated microtubule-associated protein tau in developing cortical neurons
Brion, J., Octave, J., & Couck, A. M. (1994) · Neuroscience · doi:10.1016/0306-4522(94)90533-9 · Cited by 141 · see citing works
Microtubule-associated proteins and neuronal morphogenesis
Matus, A. I. (1991) · Journal of Cell Science · doi:10.1242/jcs.1991.supplement_15.9 · Cited by 138 · see citing works
Association of poly(A) mRNA with microtubules in cultured neurons.
Bassell, G., Singer, R., & Kosik, K. (1994) · Neuron · doi:10.1016/0896-6273(94)90213-5 · Cited by 137 · see citing works
Self-citation
Plasticity of Polarization: Changing Dendrites into Axons in Neurons Integrated in Neuronal Circuits
Gomis-Rüth, S., Wierenga, C. J., & Bradke, F. (2008) · Current Biology · doi:10.1016/j.cub.2008.06.026 · Cited by 135 · see citing works
Transgenic mice overexpressing the neurotrophic factor S-100β show neuronal cytoskeletal and behavioral signs of altered aging processes: implications for Alzheimer's disease and Down's syndrome
Whitaker‐Azmitia, P. M., Wingate, M. T., Borella, A., Gerlai, R. T., Roder, J., & Azmitia, E. C. (1997) · Brain Research · doi:10.1016/s0006-8993(97)01002-0 · Cited by 135 · see citing works
NMNAT2:HSP90 Complex Mediates Proteostasis in Proteinopathies
Ali, Y., Allen, H. M., Yu, L., Li‐Kroeger, D., Bakhshizadehmahmoudi, D., Hatcher, A., Aubin, C., Xu, J., Bjorklund, N. L., Taglialatela, G., et al. (2016) · PLoS Biology · doi:10.1371/journal.pbio.1002472 · Cited by 128 · see citing works
Activity-dependent regulation of synaptic clustering in a hippocampal culture system
Kavalali, E. T., Klingauf, J., & Tsien, R. W. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.22.12893 · Cited by 128 · see citing works
Pathological proteins Tau 64 and 69 are specifically expressed in the somatodendritic domain of the degenerating cortical neurons during Alzheimer's disease
Delacourte, A., Flament, S., Dibe, E. M., Hublau, P., Sablnni�re, P., H�mon, B., Sh�rrer, V., & D�fossez, A. (1990) · Acta Neuropathologica · doi:10.1007/bf00308912 · Cited by 127 · see citing works
Synaptophysin I Controls the Targeting of VAMP2/Synaptobrevin II to Synaptic Vesicles
Pennuto, M., Bonanomi, D., Benfenati, F., & Valtorta, F. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-06-0380 · Cited by 123 · see citing works
The Golgi apparatus and the centrosome are localized to the sites of newly emerging axons in cerebellar granule neurons in vitro
Zmuda, J. F., & Rivas, R. J. (1998) · Cell Motility and the Cytoskeleton · doi:10.1002/(sici)1097-0169(1998)41:1 · Cited by 125 · see citing works
Diverse Distribution and Function of Fibrous Microtubule-Associated Proteins in the Nervous System
Schoenfeld, T. A., & Obar, R. A. (1994) · International review of cytology · doi:10.1016/s0074-7696(08)62631-5 · Cited by 124 · see citing works
Cleaved-Tau: A Biomarker of Neuronal Damage after Traumatic Brain Injury
Gabbita, S., Scheff, S., Menard, R. M., Roberts, K. N., Fugaccia, I., & Zemlan, F. (2005) · Journal of Neurotrauma · doi:10.1089/neu.2005.22.83 · Cited by 127 · see citing works
Tenascin and extracellular matrix glycoproteins: from promotion to polarization of neurite growth in vitro
Lochter, A., & Schachner, M. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-09-03986.1993 · Cited by 123 · see citing works
Differential localisation of tyrosinated, detyrosinated, and acetylated α‐tubulins in neurites and growth cones of dorsal root ganglion neurons
Robson, S. J., & Burgoyne, R. D. (1989) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.970120408 · Cited by 122 · see citing works
Methamphetamine- and Trauma-Induced Brain Injuries: Comparative Cellular and Molecular Neurobiological Substrates
Gold, M. S., Kobeissy, F. H., Wang, K., Merlo, L. J., Bruijnzeel, A. W., Krasnova, I. N., & Cadet, J. L. (2009) · Biological Psychiatry · doi:10.1016/j.biopsych.2009.02.021 · Cited by 121 · see citing works
Motor neuron mitochondrial dysfunction in spinal muscular atrophy
Miller, N., Shi, H., Zelikovich, A. S., & Ma, Y.-C. (2016) · Human Molecular Genetics · doi:10.1093/hmg/ddw262 · Cited by 119 · see citing works
Effect of chronic restraint stress and tianeptine on growth factors, growth-associated protein-43 and microtubule-associated protein 2 mRNA expression in the rat hippocampus
Kuroda, Y., & McEwen, B. S. (1998) · Molecular Brain Research · doi:10.1016/s0169-328x(98)00130-2 · Cited by 114 · see citing works
Organization of oligodendroglial membrane sheets. I: Association of myelin basic protein and 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase with cytoskeleton
Dyer, C. A., & Benjamins, J. A. (1989) · Journal of Neuroscience Research · doi:10.1002/jnr.490240211 · Cited by 112 · see citing works
Calcineurin is associated with the cytoskeleton of cultured neurons and has a role in the acquisition of polarity.
Ferreira, A., Kincaid, R., & Kosik, K. S. (1993) · Molecular Biology of the Cell · doi:10.1091/mbc.4.12.1225 · Cited by 110 · see citing works
Self-citation
A cAMP-activated pathway, including PKA and PI3K, regulates neuronal differentiation
Sanchez, S. P., Jiménez, C., Carrera, A. C., Díaz‐Nido, J., Ávila, J., & Wandosell, F. G. (2003) · Neurochemistry International · doi:10.1016/s0197-0186(03)00150-5 · Cited by 109 · see citing works
Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2™ cells using automated high-content image analysis
Harrill, J., Freudenrich, T. M., Machacek, D. W., Stice, S. L., & Mundy, W. R. (2010) · NeuroToxicology · doi:10.1016/j.neuro.2010.02.003 · Cited by 106 · see citing works
Tau Accumulation, Altered Phosphorylation, and Missorting Promote Neurodegeneration in Glaucoma
Chiasseu, M., Vargas, J. L. C., Destroismaisons, L., Velde, C. V., Leclerc, N. M., & Polo, A. D. (2016) · Journal of Neuroscience · doi:10.1523/jneurosci.3986-15.2016 · Cited by 105 · see citing works
Induction of axon-like and dendrite-like processes in neuroblastoma cells
Wu, G., Fang, Y., Lu, Z.-H., & Ledeen, R. W. (1998) · Journal of Neurocytology · doi:10.1023/a:1006910001869 · Cited by 105 · see citing works
Tau protein in cerebrospinal fluid (CSF): a blood–CSF barrier related evaluation in patients with various neurological diseases
Süßmuth, S. D., Reiber, H., & Tumani, H. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(01)01556-7 · Cited by 104 · see citing works
Basic fibroblast growth factor increases division and delays differentiation of dopamine precursors in vitro
Bouvier, M., & Mytilineou, C. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-11-07141.1995 · Cited by 104 · see citing works
Transport and diffusion of Tau protein in neurons
Scholz, T., & Mandelkow�, E. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1610-7 · Cited by 99 · see citing works
GAL4-responsive UAS- tau as a tool for studying the anatomy and development of the Drosophila central nervous system
Schneuwly, S., Hofbauer, A., Ito, K., Sass, H., & Urban, J. (1997) · Cell and Tissue Research · doi:10.1007/s004410050901 · Cited by 99 · see citing works
Ephrin-dependent growth and pruning of hippocampal axons
Gao, P.-P., Yue, Y., Cerretti, D. P., Dreyfus, C. F., & Zhou, R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.4073 · Cited by 98 · see citing works
The transport and assembly of the axonal cytoskeleton.
Hollenbeck, P. J. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.223 · Cited by 98 · see citing works
The molecular composition of neuronal microfilaments is spatially and temporally regulated
Weinberger, R. P., Schevzov, G., Jeffrey, P. L., Gordon, K., Hill, M., & Gunning, P. W. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-01-00238.1996 · Cited by 97 · see citing works
Microtubule Dynamics in Neuronal Development, Plasticity, and Neurodegeneration
Penazzi, L., Bakota, L., & Brandt, R. (2015) · International review of cytology · doi:10.1016/bs.ircmb.2015.09.004 · Cited by 94 · see citing works
PHBV microspheres as neural tissue engineering scaffold support neuronal cell growth and axon–dendrite polarization
Chen, W., & Tong, Y. W. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.09.026 · Cited by 93 · see citing works
Regional differences in glial-derived factors that promote dendritic outgrowth from mouse cortical neurons in vitro
Roux, P. L., & Reh, T. (1994) · Journal of Neuroscience · doi:10.1523/jneurosci.14-08-04639.1994 · Cited by 93 · see citing works
Tau in situ hybridization in normal and alzheimer brain: Localization in the somatodendritic compartment
Kosik, K. S., Crandall, J. E., Mufson, E. J., & Neve, R. L. (1989) · Annals of Neurology · doi:10.1002/ana.410260308 · Cited by 92 · see citing works
Self-citation
Environmental and genetic factors support the dissociation between α-synuclein aggregation and toxicity
Villar‐Piqué, A., da Fonseca, T. L., Sant’Anna, R., Szegő, É. M., Fonseca‐Ornelas, L., Pinho, R., Čarija, A., Gerhardt, E., Masaracchia, C., Gonzalez, E. A., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1606791113 · Cited by 87 · see citing works
Microtubule functions
Ávila, J. (1992) · Life Sciences · doi:10.1016/0024-3205(92)90433-p · Cited by 89 · see citing works
Tau protein kinase II has a similar characteristic to cdc2 kinase for phosphorylating neurofilament proteins
Hisanaga, S., Ishiguro, K., Uchida, T., Okumura, E., Okano, T., & Kishimoto, T. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)82437-1 · Cited by 89 · see citing works
Macroporous hydrogels derived from aqueous dynamic phase separation
Broguière, N., Husch, A., Palazzolo, G., Bradke, F., Madduri, S., & Zenobi‐Wong, M. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.01.047 · Cited by 85 · see citing works
Microtubule-associated proteins (MAPs) in the peripheral nervous system during development and regeneration
Nunez, J., & Fischer, I. (1997) · Journal of Molecular Neuroscience · doi:10.1007/bf02736834 · Cited by 83 · see citing works
Mitochondrial malic enzyme activity is much higher in mitochondria from cortical synaptic terminals compared with mitochondria from primary cultures of cortical neurons or cerebellar granule cells
McKenna, M. C., Stevenson, J., Huang, X., Tildón, J. T., Zielke, C. L., & Hopkins, I. B. (2000) · Neurochemistry International · doi:10.1016/s0197-0186(99)00148-5 · Cited by 82 · see citing works
Regionally specific properties of midbrain glia: I. Interactions with midbrain neurons
Garcia-Abreu, J., Moura‐Neto, V., de Carvalho, S. L., & Cavalcante, L. A. (1995) · Journal of Neuroscience Research · doi:10.1002/jnr.490400406 · Cited by 80 · see citing works
Tau protein: An update on structure and function
Lee, G. S. (1990) · Cell Motility and the Cytoskeleton · doi:10.1002/cm.970150402 · Cited by 80 · see citing works

An immunocytochemical analysis of the ontogeny of the microtubule-associated proteins MAP-2 and Tau in the nervous system of the rat
Ferreira, A., Busciglio, J. A., & Cáceres, A. (1987) · Developmental Brain Research · doi:10.1016/0165-3806(87)90191-x · Cited by 86 · see citing works
Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture
Caceres, A., Banker, G., & Binder, L. (1986) · The Journal of Neuroscience · doi:10.1523/jneurosci.06-03-00714.1986 · Cited by 367 · see citing works
Selective stabilization of tau in axons and microtubule-associated protein 2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons.
Hirokawa, N., Funakoshi, T., Sato-Harada, R., & Kanai, Y. (1996) · The Journal of cell biology · doi:10.1083/jcb.132.4.667 · Cited by 179 · see citing works
MAP2 is localized to the dendrites of hippocampal neurons which develop in culture
Cáceres, A., Banker, G. A., Steward, O., Binder, L. I., & Payne, M. R. (1984) · Developmental Brain Research · doi:10.1016/0165-3806(84)90167-6 · Cited by 320 · see citing works
Tau protein and the establishment of an axonal morphology
Kosik, K. S., & Cáceres, A. (1991) · Journal of Cell Science · doi:10.1242/jcs.1991.supplement_15.10 · Cited by 43 · see citing works
Self-citation

Authors: Kosik, K., & Finch, E.
Affiliations: Brigham and Women's Hospital (US)
Venue: The Journal of Neuroscience
Publisher: Society for Neuroscience
Published: 1987-10-01
Volume: 7
Issue: 10
Pages: 3142-3153
ISSN: 0270-6474 (ISSN-L)
1529-2401
DOI: 10.1523/jneurosci.07-10-03142.1987
PMC: PMC6569170
OpenAlex: W1812094318
Cited by: 368
References: 28
Citations by year: 2026: 2 · 2025: 4 · 2024: 2 · 2023: 7 · 2022: 14 · 2021: 12 · 2020: 6 · 2019: 5 · 2018: 10 · 2017: 14 · 2016: 17 · 2015: 13 · 2014: 15 · 2013: 7 · 2012: 13
FWCI: 10.43
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
creativecommons.org/licenses/by-nc-sa/4.0/ (vor, 183 d)
Locations: Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r01ag006172-05, 5k07ns000835-03)
National Institute on Aging (5 R01 AG06172)
National Institute of Neurological Disorders and Stroke (5K07 NS00835)
Topics: Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
Keywords: MAP2, tau protein, microtubule-associated proteins, dendritic compartmentalization, neurite development, neurite fasciculation, cultured neurons, microtubule cytoskeleton
MeSH terms: Animals, Axons, Cells, Cultured, Cerebral Cortex, Dendrites, Immunohistochemistry, Microtubule-Associated Proteins, Neurons, Staining and Labeling, Time Factors, tau Proteins, Rats

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MAP2 and tau segregate into dendritic and axonal domains after the elaboration of morphologically distinct neurites: an immunocytochemical study of cultured rat cerebrum

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