The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cofilin is an essential component of the yeast cortical cytoskeleton.
Open accessAbstract
We have biochemically identified the Saccharomyces cerevisiae homologue of the mammalian actin binding protein cofilin. Cofilin and related proteins isolated from diverse organisms are low molecular weight proteins (15-20 kD) that possess several activities in vitro. All bind to monomeric actin and sever filaments, and some can stably associate with filaments. In this study, we demonstrate using viscosity, sedimentation, and actin assembly rate assays that yeast cofilin (16 kD) possesses all of these properties. Cloning and sequencing of the S. cerevisiae cofilin gene (COF1) revealed that yeast cofilin is 41% identical in amino acid sequence to mammalian cofilin and, surprisingly, has homology to a protein outside the family of cofilin-like proteins. The NH2-terminal 16kD of Abp1p, a 65-kD yeast protein identified by its ability to bind to actin filaments, is 23% identical to yeast cofilin. Immunofluorescence experiments showed that, like Abp1p, cofilin is associated with the membrane actin cytoskeleton. A complete disruption of the COF1 gene was created in diploid cells. Sporulation and tetrad analysis revealed that yeast cofilin has an essential function in vivo. Although Abp1p shares sequence similarity with cofilin and has the same distribution as cofilin in the cell, multiple copies of the ABP1 gene cannot compensate for the loss of cofilin. Thus, cofilin and Abp1p are structurally related but functionally distinct components of the yeast membrane cytoskeleton.
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Cites
Distribution among tissues and intracellular localization of cofilin, a 21kDa actin-binding protein.
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Cited by
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Polarization of cell growth in yeast II. The role of the cortical actin cytoskeleton
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Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin
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Ins and outs of ADF/cofilin activity and regulation
Van Troys, M., Huyck, L., Leyman, S., Dhaese, S., Vandekerkhove, J., & Ampe, C. (2008) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2008.04.001 · Cited by 329
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Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments
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Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics
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The actin cytoskeleton of Dictyostelium: a story told by mutants
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Metadata
- Authors
- Moon, A. L., Janmey, P. A., Louie, K. A., & Drubin, D. G.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1993-01-15
- Volume
- 120
- Issue
- 2
- Pages
- 421-435
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2125510211
- Cited by
- 249
- References
- 1
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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