The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin.
Open accessAbstract
rbSec1 is a mammalian neuronal protein homologous to the yeast SEC1 gene product which is required for exocytosis. Mutations in Sec1 homologues in the nervous systems of C. elegans and D. melanogaster lead to defective neurotransmitter secretion. Biochemical studies have shown that recombinant rbSec1 binds syntaxin 1 but not SNAP-25 or synaptobrevin/VAMP, the two proteins which together with syntaxin 1 form the synaptic SNARE complex. In this study we have examined the subcellular localization of rbSec1 and the degree of interaction between rbSec1 and syntaxin 1 in situ. rbSec1, which we show here to be represented by two alternatively spliced isoforms, rbSec1A and B, has a widespread distribution in the axon and is not restricted to the nerve terminal. This distribution parallels the localization of syntaxin 1 and SNAP-25 along the entire axonal plasmalemma. rbSec1 is found in a soluble and a membrane-associated form. Although a pool of rbSec1 is present on the plasmalemma, the majority of membrane-bound rbSec1 is not associated with syntaxin 1. We also show that rbSec1 is not part of the synaptic SNARE complex or of the syntaxin 1/SNAP-25 complex we show to be present in non-synaptic regions of the axon. Thus, in spite of biochemical studies demonstrating the high affinity interaction of rbSec1 and syntaxin 1, our results indicate that rbSec1 and syntaxin 1 are not stably associated. They also suggest that the function of rbSec1, syntaxin 1, and SNAP-25 is not restricted to synaptic vesicle exocytosis at the synapse.
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Rizo, J., & Südhof, T. C. (2002) · Nature Reviews Neuroscience · doi:10.1038/nrn898 · Cited by 539
Dieck, S., Sanmartí-Vila, L., Langnaese, K., Richter, K., Kindler, S., Soyke, A., Wex, H., Smalla, K.-H., Kämpf, U., Fränzer, J.-T., et al. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.2.499 · Cited by 482
Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming
Betz, A., Thakur, P., Junge, H. J., Ashery, U., Rhee, J.-S., Scheuss, V., Rosenmund, C., Rettig, J., & Brose, N. (2001) · Neuron · doi:10.1016/s0896-6273(01)00272-0 · Cited by 425
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Metadata
- Authors
- Garcia, E. P., McPherson, P. S., Chilcote, T. J., Takei, K., & De Camilli, P.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1995-04-01
- Volume
- 129
- Issue
- 1
- Pages
- 105-120
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2162740393
- Cited by
- 266
- References
- 62
- FWCI
- 9.96
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin. https://app.cochat.ai/papers/10.1083/jcb.129.1.105
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- Garcia, E. P., McPherson, P. S., Chilcote, T. J., Takei, K., & De Camilli, P. (1995). rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin. The Journal of cell biology. https://doi.org/10.1083/jcb.129.1.105
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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