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Cited by 482Neuroscience and Neuropharmacology ResearchCellular transport and secretionGenetics and Neurodevelopmental Disorders

Bassoon, a novel zinc-finger CAG/glutamine-repeat protein selectively localized at the active zone of presynaptic nerve terminals

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Dieck, Susannetom, Sanmartí-Vila, Lydia, Langnaese, Kristina, Richter, Karin, Kindler, Stefan, Soyke, Antje, Wex, Heike, Smalla, Karl-Heinz +5 more·1998·The Journal of Cell Biology
DOI 10.1083/jcb.142.2.499PMC PMC2133055
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Abstract

The molecular architecture of the cytomatrix of presynaptic nerve terminals is poorly understood. Here we show that Bassoon, a novel protein of >400,000 Mr, is a new component of the presynaptic cytoskeleton. The murine bassoon gene maps to chromosome 9F. A comparison with the corresponding rat cDNA identified 10 exons within its protein-coding region. The Bassoon protein is predicted to contain two double-zinc fingers, several coiled-coil domains, and a stretch of polyglutamines (24 and 11 residues in rat and mouse, respectively). In some human proteins, e.g., Huntingtin, abnormal amplification of such poly-glutamine regions causes late-onset neurodegeneration. Bassoon is highly enriched in synaptic protein preparations. In cultured hippocampal neurons, Bassoon colocalizes with the synaptic vesicle protein synaptophysin and Piccolo, a presynaptic cytomatrix component. At the ultrastructural level, Bassoon is detected in axon terminals of hippocampal neurons where it is highly concentrated in the vicinity of the active zone. Immunogold labeling of synaptosomes revealed that Bassoon is associated with material interspersed between clear synaptic vesicles, and biochemical studies suggest a tight association with cytoskeletal structures. These data indicate that Bassoon is a strong candidate to be involved in cytomatrix organization at the site of neurotransmitter release.

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  • Piccolo and bassoon are associated with active zones in vertebrates.

    “In addition to these five core active zone proteins, piccolo and bassoon (two large homologous proteins) are associated in vertebrates with active zones (tom Dieck et al., 1998; Wang et al., 1999; Fenster et al., 2000; Limbach et al., 2011), and proteins related to C. elegans SYD-1 are important for the assembly of active zones in invertebrates (Hallam et al., 2002; Patel et al., 2006; Owald et al”

    From The Presynaptic Active Zone (2012) · cites this paper as tom Dieck et al., 1998

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Authors
Dieck, S., Sanmartí-Vila, L., Langnaese, K., Richter, K., Kindler, S., Soyke, A., Wex, H., Smalla, K.-H., Kämpf, U., Fränzer, J.-T., Stumm, M., Garner, C. C., & Gundelfinger, E. D.
Affiliations
  • *Leibniz Institute for Neurobiology, D-39118 Magdeburg, Germany; ‡Institute for Cellular Biochemistry and Clinical Neurobiology, University of Hamburg, D-20246 Hamburg, Germany; §Institute for Human Genetics, Medical Faculty, Otto von Guericke University, D-39120 Magdeburg, Germany; ‖Institute for Pharmacology and Toxicology, Medical Faculty, Otto von Guericke University, D-39120 Magdeburg, Germany; and ¶Department of Neurobiology, University of Alabama at Birmingham, South Birmingham, Alabama 35213-0021
  • Universität Hamburg (DE)
ORCID
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1998-07-27
Volume
142
Issue
2
Pages
499-509
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.142.2.499
PMC
PMC2133055
OpenAlex
W1944633888
Cited by
482
References
1
Citations by year
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FWCI
6.33
Type
journal article
Language
en
Open access
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • W. M. Keck Foundation
  • Deutsche Forschungsgemeinschaft (SFB 426)
  • National Institutes of Health (5p50hd032901-05, P50 HD32901)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (P50 HD32901)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
bassoon, active zone, presynaptic terminals, zinc finger proteins, polyglutamine repeat, coiled-coil domain, synaptophysin, piccolo, synaptic vesicles, neurotransmitter release, hippocampal neurons, gene mapping, huntingtin
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cloning, Molecular, Exons, Hippocampus, Humans, Molecular Sequence Data, Molecular Weight, Nerve Tissue Proteins, Peptides, Microscopy, Immunoelectron, Zinc Fingers, Sequence Homology, Amino Acid, Presynaptic Terminals, DNA, Complementary, Trinucleotide Repeats, Mice, Rats

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