Mdm12p, a Component Required for Mitochondrial Inheritance That Is Conserved between Budding and Fission Yeast
Open accessAbstract
Saccharomyces cerevisiae cells lacking the MDM12 gene product display temperature-sensitive growth and possess abnormally large, round mitochondria that are defective for inheritance by daughter buds. Analysis of the wild-type MDM12 gene revealed its product to be a 31-kD polypeptide that is homologous to a protein of the fission yeast Schizosaccharomyces pombe. When expressed in S. cerevisiae, the S. pombe Mdm12p homolog conferred a dominant-negative phenotype of giant mitochondria and aberrant mitochondrial distribution, suggesting partial functional conservation of Mdm12p activity between budding and fission yeast. The S. cerevisiae Mdm12p was localized by indirect immunofluorescence microscopy and by subcellular fractionation and immunodetection to the mitochondrial outer membrane and displayed biochemical properties of an integral membrane protein. Mdm12p is the third mitochondrial outer membrane protein required for normal mitochondrial morphology and distribution to be identified in S. cerevisiae and the first such mitochondrial component that is conserved between two different species.
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Deletion mutants of the ERMES core complex display defects in the segregation of mtDNA into daughter cells, resulting in the loss of mitochondria over several generations of growth in Saccharomyces cerevisiae.
“In particular, deletion mutants of the ERMES core complex display defects in the segregation of mtDNA into daughter cells, resulting in the loss of mitochondria over several generations of growth in S. cerevisiae [27,29,30,45].”
From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [27]
The Results section reports that mdm12-null cells in S. cerevisiae frequently fail to transmit mitochondria to daughter buds; the Discussion also describes cells lacking mitochondria. The paper measures mitochondrial distribution and respiratory function, but does not establish defective mtDNA segregation or loss of mitochondria over several generations of growth. It discusses Mdm10p, Mmm1p, and Mdm12p as proteins that may function together, rather than identifying an ERMES core complex. (LLM, conf=0.87)
Experimental — accuracy not yet measuredThe ERMES complex is involved in mitochondrial fission, the distribution of mtDNA, and mitophagy.
“The ERMES complex is also involved in mitochondrial fission, the distribution of mtDNA, and mitophagy [27,28,29,30,31,32,33].”
From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [27]
The Discussion (page not provided) reports that Mdm12p is required for normal mitochondrial morphology and inheritance, and suggests that it may function in a complex with Mdm10p and Mmm1p. It does not establish a role for that complex in mitochondrial fission, mtDNA distribution, or mitophagy; mitochondrial inheritance here refers to transfer of mitochondria into daughter buds. (LLM, conf=0.87)
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- Authors
- Berger, K. H., Sogo, L. F., & Yaffe, M. P.
- Affiliations
- University of California San Diego (US)
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-02-10
- Volume
- 136
- Issue
- 3
- Pages
- 545-553
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2134291
- OpenAlex
- W2136570326
- Cited by
- 197
- References
- 37
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 4 · 2023: 3 · 2022: 1 · 2021: 5 · 2020: 5 · 2019: 5 · 2018: 5 · 2017: 11 · 2016: 12 · 2015: 4 · 2014: 9 · 2013: 7 · 2012: 7
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM044614, GM16173, GM44614, F32GM016173)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- mitochondrial inheritance, mitochondrial morphology, mitochondrial distribution, mitochondrial outer membrane proteins, Saccharomyces cerevisiae, Schizosaccharomyces pombe, temperature-sensitive growth, dominant negative phenotype, giant mitochondria
- MeSH terms
- Base Sequence, Cell Membrane, DNA, Fungal, Fungal Proteins, Membrane Proteins, Mitochondria, Molecular Sequence Data, Phenotype, Repressor Proteins, Saccharomyces cerevisiae, Schizosaccharomyces, Spores, Fungal, Sequence Homology, Amino Acid, Saccharomyces cerevisiae Proteins
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