Cited by 249Mitochondrial Function and PathologyFungal and yeast genetics researchAutophagy in Disease and Therapy

MMM1 Encodes a Mitochondrial Outer Membrane Protein Essential for Establishing and Maintaining the Structure of Yeast Mitochondria

Open access
Burgess, S M, Delannoy, M, Jensen, R E·1994·The Journal of Cell Biology

Abstract

In the yeast Saccharomyces cerevisiae, mitochondria are elongated organelles which form a reticulum around the cell periphery. To determine the mechanism by which mitochondrial shape is established and maintained, we screened yeast mutants for those defective in mitochondrial morphology. One of these mutants, mmm1, is temperature-sensitive for the external shape of its mitochondria. At the restrictive temperature, elongated mitochondria appear to quickly collapse into large, spherical organelles. Upon return to the permissive temperature, wild-type mitochondrial structure is restored. The morphology of other cellular organelles is not affected in mmm1 mutants, and mmm1 does not disrupt normal actin or tubulin organization. Cells disrupted in the MMM1 gene are inviable when grown on nonfermentable carbon sources and show abnormal mitochondrial morphology at all temperatures. The lethality of mmm1 mutants appears to result from the inability to segregate the aberrant-shaped mitochondria into daughter cells. Mitochondrial structure is therefore important for normal cell function. Mmm1p is located in the mitochondrial outer membrane, with a large carboxyl-terminal domain facing the cytosol. We propose that Mmm1p maintains mitochondria in an elongated shape by attaching the mitochondrion to an external framework, such as the cytoskeleton.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1083/jcb.126.6.1375

Cites

72 of 72 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The ERMES complex is involved in mitochondrial fission, the distribution of mtDNA, and mitophagy.

    “The ERMES complex is also involved in mitochondrial fission, the distribution of mtDNA, and mitophagy [27,28,29,30,31,32,33].”

    From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [28]

    The Results (pp. 1378, 1386–1387) show that MMM1 mutants have altered mitochondrial shape and impaired transmission of DNA-containing mitochondria to daughter buds. The Discussion raises mitochondrial division as a possible mechanism but does not establish a role in fission. Our copy does not discuss the ERMES complex or mitophagy, or directly establish a role in mtDNA distribution. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Burgess, S. M., Delannoy, M., & Jensen, R. E.
Affiliations
Johns Hopkins University (US)
Venue
The Journal of Cell Biology
Published
1994-09-15
Volume
126
Issue
6
Pages
1375-1391
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W2166125801
Cited by
249
References
72
Citations by year
2026: 1 · 2025: 3 · 2024: 7 · 2023: 6 · 2022: 3 · 2021: 6 · 2020: 8 · 2019: 3 · 2018: 4 · 2017: 9 · 2016: 10 · 2015: 8 · 2014: 10 · 2013: 7 · 2012: 6
FWCI
3.80
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • U.S. Public Health Service (5T32GN07445)
  • National Institute of General Medical Sciences (R01GM46803, R01GM046803)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
mitochondrial outer membrane proteins, mitochondrial morphology, Saccharomyces cerevisiae, temperature-sensitive mutants, mitochondrial segregation, organelle structure
MeSH terms
Amino Acid Sequence, Base Sequence, Carbon, Cell Membrane Permeability, Fungal Proteins, Genes, Fungal, Intracellular Membranes, Membrane Proteins, Mitochondria, Molecular Sequence Data, Mutation, Saccharomyces cerevisiae, Temperature, Saccharomyces cerevisiae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review