Regulation of Mitochondrial Morphology and Inheritance by Mdm10p, a Protein of the Mitochondrial Outer Membrane
Open accessAbstract
Yeast cells with the mdm10 mutation possess giant spherical mitochondria and are defective for mitochondrial inheritance. The giant mitochondria display classical features of mitochondrial ultrastructure, yet they appear incapable of movement or division. Genetic analysis indicated that the mutant phenotypes resulted from a single nuclear mutation, and the isolated MDM10 gene restored wild-type mitochondrial distribution and morphology when introduced into mutant cells. MDM10 encodes a protein of 56.2 kD located in the mitochondrial outer membrane. Depletion of Mdm10p from cells led to a condensation of normally extended, tubular mitochondria into giant spheres, and reexpression of the protein resulted in a rapid restoration of normal mitochondrial morphology. These results demonstrate that Mdm10p can control mitochondrial morphology, and that it plays a role in the inheritance of mitochondria.
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The ERMES complex is involved in mitochondrial fission, the distribution of mtDNA, and mitophagy.
“The ERMES complex is also involved in mitochondrial fission, the distribution of mtDNA, and mitophagy [27,28,29,30,31,32,33].”
From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [29]
The discussion (p. 1371) reports that mdm10 mutant mitochondria are generally defective in division and inheritance, supporting the fission-related part of the claim. The paper studies Mdm10p, but our copy does not establish a role for the ERMES complex in mtDNA distribution or mitophagy. (LLM, conf=0.88)
Experimental — accuracy not yet measuredDeletion mutants of the ERMES core complex display defects in the segregation of mtDNA into daughter cells, resulting in the loss of mitochondria over several generations of growth in Saccharomyces cerevisiae.
“In particular, deletion mutants of the ERMES core complex display defects in the segregation of mtDNA into daughter cells, resulting in the loss of mitochondria over several generations of growth in S. cerevisiae [27,29,30,45].”
From In Candida glabrata, ERMES Component GEM1 Controls Mitochondrial Morphology, mtROS, and Drug Efflux Pump Expression, Resulting in Azole Susceptibility (2023) · cites this paper as [29]
The Results (pp. 1363–1364) report that an MDM10 mutant has defective mitochondrial inheritance: 60–65% of daughter buds lacked mitochondria under the stated growth conditions. The paper studies MDM10 rather than deletion mutants of the ERMES core complex as a group, and it does not establish defective mtDNA segregation or loss of mitochondria over several generations. (LLM, conf=0.86)
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- Authors
- Sogo, L. F., & Yaffe, M. P.
- Affiliations
- University of California San Diego (US)
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1994-09-15
- Volume
- 126
- Issue
- 6
- Pages
- 1361-1373
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2290945
- OpenAlex
- W2140850020
- Cited by
- 284
- References
- 42
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 8 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 10 · 2019: 4 · 2018: 7 · 2017: 8 · 2016: 13 · 2015: 8 · 2014: 11 · 2013: 6 · 2012: 10
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM044614, GM44614)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- mitochondrial morphology, mitochondrial inheritance, mitochondrial outer membrane, giant mitochondria, mitochondrial division, mitochondrial distribution, Saccharomyces cerevisiae, nuclear mutations, mitochondrial ultrastructure
- MeSH terms
- Amino Acid Sequence, Base Sequence, Fungal Proteins, Intracellular Membranes, Membrane Proteins, Mitochondria, Molecular Sequence Data, Mutation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
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