The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Leukemia Inhibitory Factor–dependent Transcriptional Activation in Embryonic Stem Cells
Open accessAbstract
STAT transcription factors are induced by a number of growth factors and cytokines. Within minutes of induction, the STAT proteins are phosphorylated on tyrosine and serine residues and translocated to the nucleus, where they bind to their DNA targets. The leukemia inhibitory factor (LIF) mediates pleiotropic and sometimes opposite effects both in vivo and in cultured cells. It is known, for example, to prevent differentiation of embryonic stem (ES) cells in vitro. To get insights into LIF-regulated signaling in ES cells, we have analyzed protein-binding and transcriptional properties of STAT recognition sites in ES cells cultivated in the presence and in the absence of LIF. We have detected a specific LIF-regulated DNA-binding activity implicating the STAT3 protein. We show that STAT3 phosphorylation is essential for this LIF-dependent DNA-binding activity. The possibility that ERK2 or a closely related protein kinase, whose activity is modulated in a LIF-dependent manner, contributes to this phosphorylation is discussed. Finally, we show that the multimerized STAT3-binding DNA element confers LIF responsiveness to a minimal thymidine kinase promoter. This, together with our observation that overexpression of STAT3 dominant-negative mutants abrogates this LIF responsiveness, clearly indicates that STAT3 is involved in LIF-regulated transcriptional events in ES cells. Finally, stable expression of such a dominant negative mutant of STAT3 induces morphological differentiation of ES cells despite continuous LIF supply. Our results suggest that STAT3 is a critical target of the LIF signaling pathway, which maintains pluripotent cell proliferation.
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Cites
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Liver Failure and Defective Hepatocyte Regeneration in Interleukin-6-Deficient Mice
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Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality
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Association and Activation of Jak-Tyk Kinases by CNTF-LIF-OSM-IL-6 β Receptor Components
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Enhanced DNA-Binding Activity of a Stat3-Related Protein in Cells Transformed by the Src Oncoprotein
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“Activation of these kinases occurs as a result of LIFinduced dimerization of the receptor components (gp130LIFR b ) and leads to their phosphorylation (22, 31, 34, 52, 53).”
BackgroundInfluentialShuai, K., Horvath, C. M., Huang, L. H. T., Qureshi, S. A., Cowburn, D., & Darnell, J. E. (1994) · Cell · doi:10.1016/0092-8674(94)90357-3 · Cited by 807
Activation and Association of Stat3 with Src in v-Src-Transformed Cell Lines
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Cited by
Levy, D. E., & Lee, C.-K. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215650 · Cited by 734
Takeda, K., Kaisho, T., Yoshida, N., Takeda, J., Kishimoto, T., & Akira, S. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1500168 · Cited by 616
Suppression of SHP-2 and ERK Signalling Promotes Self-Renewal of Mouse Embryonic Stem Cells
Burdon, T., Stracey, C., Chambers, I., Nichols, J., & Smith, A. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9265 · Cited by 585
Takeda, K., Kaisho, T., Yoshida, N., Takeda, J., Kishimoto, T., & Akira, S. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.9.4652 · Cited by 584
STAT3 Is Required for Proliferation and Maintenance of Multipotency in Glioblastoma Stem Cells
Sherry, M. M., Reeves, A., Wu, J. K., & Cochran, B. H. (2009) · Stem Cells · doi:10.1002/stem.185 · Cited by 478
Metadata
- Authors
- Boeuf, H., Hauss, C., Graeve, F. D., Baran, N., & Kedinger, C.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1997-09-22
- Volume
- 138
- Issue
- 6
- Pages
- 1207-1217
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2015921568
- Cited by
- 227
- References
- 9
- FWCI
- 3.87
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Boeuf, H., Hauss, C., Graeve, F. D., Baran, N., & Kedinger, C. (1997). Leukemia Inhibitory Factor–dependent Transcriptional Activation in Embryonic Stem Cells. The Journal of Cell Biology. https://doi.org/10.1083/jcb.138.6.1207
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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