The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
EGF-R signaling through Fyn kinase disrupts the function of integrin α6β4 at hemidesmosomes
Open accessAbstract
We have examined the mechanism and functional significance of hemidesmosome disassembly during normal epithelial cell migration and squamous carcinoma invasion. Our findings indicate that a fraction of EGF receptor (EGF-R) combines with the hemidesmosomal integrin α6β4 in both normal and neoplastic keratinocytes. Activation of the EGF-R causes tyrosine phosphorylation of the β4 cytoplasmic domain and disruption of hemidesmosomes. The Src family kinase inhibitors PP1 and PP2 prevent tyrosine phosphorylation of β4 and disassembly of hemidesmosomes without interfering with the activation of EGF-R. Coimmunoprecipitation experiments indicate that Fyn and, to a lesser extent, Yes combine with α6β4. By contrast, Src and Lck do not associate with α6β4 to a significant extent. A dominant negative form of Fyn, but not Src, prevents tyrosine phosphorylation of β4 and disassembly of hemidesmosomes. These observations suggest that the EGF-R causes disassembly of hemidesmosomes by activating Fyn, which in turn phosphorylates the β4 cytoplasmic domain. Neoplastic cells expressing dominant negative Fyn display increased hemidesmosomes and migrate poorly in vitro in response to EGF. Furthermore, dominant negative Fyn decreases the ability of squamous carcinoma cells to invade through Matrigel in vitro and to form lung metastases following intravenous injection in nude mice. These results suggest that disruption of hemidesmosomes mediated by Fyn is a prerequisite for normal keratinocyte migration and squamous carcinoma invasion.
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Cites
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Src family kinases are required for integrin but not PDGFR signal transduction
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Self-citationActivation of Phosphoinositide 3-OH Kinase by the α6β4 Integrin Promotes Carcinoma Invasion
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Structure and Function of Hemidesmosomes: More Than Simple Adhesion Complexes
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Lechler, T., & Fuchs, E. (2005) · Nature · doi:10.1038/nature03922 · Cited by 998
Adhesion signaling – crosstalk between integrins, Src and Rho
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β-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex for Survival and Tumorigenesis
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β4 Integrin Amplifies ErbB2 Signaling to Promote Mammary Tumorigenesis
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Metadata
- Authors
- Mariotti, A., Kedeshian, P. A., Dans, M., Curatola, A. M., Gagnoux-Palacios, L., & Giancotti, F. G.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-10-29
- Volume
- 155
- Issue
- 3
- Pages
- 447-458
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2146958914
- Cited by
- 305
- References
- 55
- FWCI
- 7.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Mariotti, A., Kedeshian, P. A., Dans, M., Curatola, A. M., Gagnoux-Palacios, L., & Giancotti, F. G. (2001). EGF-R signaling through Fyn kinase disrupts the function of integrin α6β4 at hemidesmosomes. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200105017
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