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Cited by 427Cellular transport and secretionLipid Membrane Structure and BehaviorExtracellular vesicles in disease

Plasma membrane deformation by circular arrays of ESCRT-III protein filaments

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Hanson, Phyllis I., Roth, Robyn, Lin, Yuan, Heuser, John E.·2008·The Journal of Cell Biology
DOI 10.1083/jcb.200707031PMC PMC2213594
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Abstract

Endosomal sorting complex required for transport III (ESCRT-III) proteins function in multivesicular body biogenesis and viral budding. They are recruited from the cytoplasm to the membrane, where they assemble into large complexes. We used “deep-etch” electron microscopy to examine polymers formed by the ESCRT-III proteins hSnf7-1 (CHMP4A) and hSnf7-2 (CHMP4B). When overexpressed, these proteins target to endosomes and the plasma membrane. Both hSnf7 proteins assemble into regular approximately 5-nm filaments that curve and self-associate to create circular arrays. Binding to a coexpressed adenosine triphosphate hydrolysis–deficient mutant of VPS4B draws these filaments together into tight circular scaffolds that bend the membrane away from the cytoplasm to form buds and tubules protruding from the cell surface. Similar buds develop in the absence of mutant VPS4B when hSnf7-1 is expressed without its regulatory C-terminal domain. We demonstrate that hSnf7 proteins form novel membrane-attached filaments that can promote or stabilize negative curvature and outward budding. We suggest that ESCRT-III polymers delineate and help generate the luminal vesicles of multivesicular bodies.

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  • Key ESCRT-III components, such as CHMP2 and CHMP4, and VPS4 ATPase, are recruited to the budding site at the plasma membrane.

    “key ESCRT-III components, such as charged multivesicular body proteins 2 and 4 (CHMP2 and CHMP4), and the vacuolar protein sorting-associated protein 4 ATPase (VPS4 ATPase), are recruited to the budding site at the plasma membrane”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Hanson et al. 2008

    The Results (page unavailable) show CHMP4 filaments and a coexpressed hydrolysis-defective VPS4B mutant at plasma-membrane arrays associated with buds. The Discussion (page unavailable) says ESCRT machinery, probably including ESCRT-III, is recruited to the plasma membrane. The paper does not establish that CHMP2 is recruited to those budding sites, and its direct budding-site observation uses mutant rather than unqualified VPS4. (LLM, conf=0.87)

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Cited by

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Authors
Hanson, P. I., Roth, R., Lin, Y., & Heuser, J. E.
Affiliations
Washington University in St. Louis (US)
ORCID
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2008-01-21
Volume
180
Issue
2
Pages
389-402
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.200707031
PMC
PMC2213594
OpenAlex
W2166456692
Cited by
427
References
1
Citations by year
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FWCI
21.02
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
Funders
National Institute of General Medical Sciences (R01GM029647)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
ESCRT-III, CHMP4A, CHMP4B, VPS4B, membrane curvature, plasma membrane deformation, multivesicular body biogenesis, viral budding, membrane budding, membrane tubules, protein polymers, endosomes
MeSH terms
Animals, Carrier Proteins, Cell Line, Cell Membrane, Chlorocebus aethiops, Humans, Microscopy, Electron, Mutation, Polymers, Endosomes, Transfection, COS Cells, Vesicular Transport Proteins, Endosomal Sorting Complexes Required for Transport

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