Cytoplasmic actin in neuronal processes as a possible mediator of synaptic plasticity.
Open accessAbstract
We have demonstrated that, after permeation with saponin and decoration with S-1 myosin subfragment, the cytoplasmic actin is organized in filaments in dendritic spines, dendrites, and axon terminals of the dentate molecular layer. The filaments are associated with the plasma membrane and the postsynaptic density with their barbed ends and also in parallel with periodical cross bridges. In the spine stalks and dendrites, the actin filaments are organized in long strands. Given the contractile properties of actin, these results suggest that the cytoplasmic actin may be involved in various forms of experimentally induced synaptic plasticity by changing the shape or volume of the pre- and postsynaptic side and by retracting and sprouting synapses.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Actin filament density is much greater in spines than in the adjacent dendritic shaft or presynaptic terminal.
“direct observations of actin filaments identified by heavy meromyosin labeling confirm a much greater density of actin filaments in spines compared with that in the adjacent dendritic shaft or presynaptic terminal (Fifkova and Delay, 1982).”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Fifkova and Delay, 1982
The Dendrites section (p. 347) says the filament network in dendrites is far less dense than in spines, supporting the comparison with the dendritic shaft. The Axon Terminals section (p. 347) describes a network similar to that in spines but does not establish a density comparison with presynaptic terminals. The Methods describe labeling with myosin S-1 subfragment, not heavy meromyosin. (LLM, conf=0.90)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cited by
Sorted by citations
Structure and function of dendritic spines
Nimchinsky, E. A., Sabatini, B. L., & Svoboda, K. (2002) · Annual Review of Physiology · doi:10.1146/annurev.physiol.64.081501.160008 · Cited by 1,208 · see citing works
Hoover, B. R., Reed, M. N., Su, J., Penrod, R. D., Kotilinek, L. A., Grant, M. K., Pitstick, R., Carlson, G. A., Lanier, L. M., Yuan, L.-L., et al. (2010) · Neuron · doi:10.1016/j.neuron.2010.11.030 · Cited by 1,118 · see citing works
Rapid Actin-Based Plasticity in Dendritic Spines
Fischer, M., Kaech, S., Knutti, D., & Matus, A. (1998) · Neuron · doi:10.1016/s0896-6273(00)80467-5 · Cited by 905 · see citing works
Arendt, T., Stieler, J. T., & Holzer, M. (2016) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2016.08.018 · Cited by 609 · see citing works
Actin Cytoskeleton Regulation in Neuronal Morphogenesis and Structural Plasticity
Luo, L. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.031802.150501 · Cited by 658 · see citing works
Scofield, M. D., Heinsbroek, J. A., Gipson, C. D., Kupchik, Y. M., Spencer, S., Smith, A. C. W., Roberts-Wolfe, D., & Kalivas, P. W. (2016) · Pharmacological Reviews · doi:10.1124/pr.116.012484 · Cited by 598 · see citing works
Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin
Halpain, S., Hipolito, A., & Saffer, L. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-23-09835.1998 · Cited by 408 · see citing works
“Immunoelectron microscopy studies demonstrated that actin is highly enriched at spines as compared with the surrounding neuropil (Matus et al., 1982; Cohen et al., 1985), and direct observations of actin filaments identified by heavy meromyosin labeling confirm a much greater density of actin filaments in spines compa…”
BackgroundDevelopmental regulation of spine motility in the mammalian central nervous system
Dunaevsky, A., Tashiro, A., Majewska, A., Mason, C., & Yuste, R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.23.13438 · Cited by 412 · see citing works
Korobova, F., & Svitkina, T. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-07-0596 · Cited by 399 · see citing works
A Role of Actin Filament in Synaptic Transmission and Long-Term Potentiation
Kim, C.-H., & Lisman, J. E. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-11-04314.1999 · Cited by 386 · see citing works
Bonhoeffer, T., & Yuste, R. (2002) · Neuron · doi:10.1016/s0896-6273(02)00906-6 · Cited by 337 · see citing works
Dendritic spines: from structure to in vivo function
Rochefort, N. L., & Konnerth, A. (2012) · The EMBO Reports · doi:10.1038/embor.2012.102 · Cited by 299 · see citing works
Hasbani, M. J., Schlief, M. L., Fisher, D. A., & Goldberg, M. P. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-07-02393.2001 · Cited by 208 · see citing works
Glutamatergic synapses onto hippocampal interneurons: precision timing without lasting plasticity
McBain, C. J., Freund, T. F., & Mody, I. (1999) · Trends in Neurosciences · doi:10.1016/s0166-2236(98)01347-2 · Cited by 113 · see citing works
Cohen, R. S., Chung, S. K., & Pfaff, D. W. (1985) · Cellular and Molecular Neurobiology · doi:10.1007/bf00711012 · Cited by 114 · see citing works
Diffusion laws in dendritic spines
Holcman, D., & Schuss, Z. (2011) · The Journal of Mathematical Neuroscience · doi:10.1186/2190-8567-1-10 · Cited by 50 · see citing works
- Authors
- Fifková, E., & Delay, R. J.
- Affiliations
- Johns Hopkins University (US)
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1982-10-01
- Volume
- 95
- Issue
- 1
- Pages
- 345-350
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2112353
- OpenAlex
- W2011734517
- Cited by
- 309
- References
- 0
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 6 · 2017: 6 · 2016: 18 · 2015: 8 · 2014: 17 · 2013: 12 · 2012: 11
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- University of Washington
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- cytoplasmic actin, F-actin, synaptic plasticity, dendritic spines, postsynaptic density, axon terminals, dendrites, synaptic morphology
- MeSH terms
- Actins, Animals, Axons, Cytoskeleton, Dendrites, Hippocampus, Neuronal Plasticity, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
