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A Role for Endogenous Transforming Growth Factor β1 in Langerhans Cell Biology: The Skin of Transforming Growth Factor β1 Null Mice Is Devoid of Epidermal Langerhans Cells

Borkowski, Teresa A., Letterio, John J., Farr, Andrew G., Udey, Mark C.·1996·The Journal of Experimental Medicine
DOI 10.1084/jem.184.6.2417PMC PMC2196398
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Abstract

Transforming growth factor β1 (TGF-β1) regulates leukocytes and epithelial cells. To determine whether the pleiotropic effects of TGF-β1, a cytokine that is produced by both keratinocytes and Langerhans cells (LC), extend to epidermal leukocytes, we characterized LC (the epidermal contingent of the dendritic cell [DC] lineage) and dendritic epidermal T cells (DETC) in TGF-β1 null (TGF-β1 −/−) mice. I-A+ LC were not detected in epidermal cell suspensions or epidermal sheets prepared from TGF-β1 −/− mice, and epidermal cell suspensions were devoid of allostimulatory activity. In contrast, TCR-γδ+ DETC were normal in number and appearance in TGF-β1 −/− mice and, importantly, DETC represented the only leukocytes in the epidermis. Immunolocalization studies revealed CD11c+ DC in lymph nodes from TGF-β1 −/− mice, although gp40+ DC were absent. Treatment of TGF-β1 −/− mice with rapamycin abrogated the characteristic inflammatory wasting syndrome and prolonged survival indefinitely, but did not result in population of the epidermis with LC. Thus, the LC abnormality in TGF-β1 −/− mice is not a consequence of inflammation in skin or other organs, and LC development is not simply delayed in these animals. We conclude that endogenous TGF-β1 is essential for normal murine LC development or epidermal localization.

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  • Endogenous TGF-β1 is essential for normal murine Langerhans cell development and TGF-β1 acts directly on Langerhans cells through an autocrine/paracrine loop.

    “it has been reported that endogenous TGF-β1 is essential for normal murine Langerhans cell development35 and TGF-β1 acts directly on Langerhans cells through an autocrine/paracrine loop36.”

    From Identification of a unique TGF-β–dependent molecular and functional signature in microglia (2014) · cites this paper as [35]

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Research works printed but not linked: 30

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Authors
Borkowski, T. A., Letterio, J. J., Farr, A. G., & Udey, M. C.
Affiliations
From the *Dermatology Branch and ‡the Laboratory of Chemoprevention, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1908; and the §Department of Biological Structure, University of Washington, Seattle, Washington 98195
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
1996-12-01
Volume
184
Issue
6
Pages
2417-2422
ISSN
  • 0022-1007
  • 1540-9538
DOI
10.1084/jem.184.6.2417
PMC
PMC2196398
Cited by
402
References
0
Language
en

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