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Isolation, Structure Elucidation, and Synthesis of a Macrophage Stimulatory Lipopeptide from Mycoplasma fermentans Acting at Picomolar Concentration
Open accessAbstract
Macrophages are typically stimulated by components of microbial cell walls. Surprisingly, cell wall–less mycoplasmas can also very efficiently stimulate macrophages. We showed recently that mycoplasma-derived lipopeptides constitute the active principle. We have now isolated a clone of Mycoplasma fermentans expressing mainly one macrophage-stimulating lipopeptide. This lipopeptide was detergent-extracted and isolated by reversed-phase high-performance liquid chromotography, using nitric oxide release from C3H/HeJ mouse macrophages as bioassay for detection. In contrast to “conventional” bacterial lipoproteins, this lipopeptide had a free NH2 terminus. Amino acid composition, sequence, and the molecular weight of 2,163.3 are consistent with the following structure: S-(2,3-bisacyloxypropyl)cysteine-GNNDESNISFKEK with one mole C16:0, and a further mole of a mixture of C18:0 and C18:1 fatty acid per lipopeptide molecule. The sequence could not be found in either the protein identification resource nor the Swiss Prot data bank. We named this 2-kD lipopeptide, macrophage-activating lipopeptide-2 (MALP-2). Synthetic dipalmitoyl MALP-2 and mycoplasma-derived MALP-2 were compared with the bioassay. Both lipopeptides showed an identical dose dependency with a half-maximal response at 10−11 M concentration. MALP-2 may be one of the most potent natural macrophage stimulators besides endotoxin.
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PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
A gas-liquid solid phase peptide and protein sequenator.
Hewick, R. M., Hunkapiller, M. W., Hood, L. E., & Dreyer, W. J. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)43377-7 · Cited by 2,218
Complete Sequence Analysis of the Genome of the Bacterium Mycoplasma Pneumoniae
Himmelreich, R., Hilbert, H., Plagens, H., Pirkl, E., Li, B.-C., & Herrmann, R. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.22.4420 · Cited by 1,145
THE QUANTITATIVE HISTOCHEMISTRY OF BRAIN
Lowry, O. H., Roberts, N. R., Leiner, K. Y., Wu, M.-L., & Farr, A. L. (1954) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71241-6 · Cited by 1,019
THE QUANTITATIVE HISTOCHEMISTRY OF THE RETINA
Lowry, O. H., Roberts, N. R., & Lewis, C. (1956) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)65313-x · Cited by 911
Host factors and the pathogenesis of HIV-induced disease
Fauci, A. S. (1996) · Nature · doi:10.1038/384529a0 · Cited by 829
In vivo priming of virus-specific cytotoxic T lymphocytes with synthetic lipopeptide vaccine
Deres, K., Schild, H., Wiesmüller, K.-H., Jung, G., & Rammensee, H.-G. (1989) · Nature · doi:10.1038/342561a0 · Cited by 631
Self-citationInfluence of Matrix Solution Conditions on the MALDI-MS Analysis of Peptides and Proteins
Cohen, S. L., & Chait, B. T. (1996) · Analytical Chemistry · doi:10.1021/ac9507956 · Cited by 547
Hantke, K., & Braun, V. (1973) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1973.tb02757.x · Cited by 444
Henderson, B., Poole, S., & Wilson, M. (1996) · Microbiological Reviews · doi:10.1128/mmbr.60.2.316-341.1996 · Cited by 419
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Molecular Biology and Pathogenicity of Mycoplasmas
Razin, S., Yogev, D., & Naot, Y. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.4.1094-1156.1998 · Cited by 1,930
A Novel Host-Parasite Lipid Cross-talk
van der Kleij, D., Latz, E., Brouwers, J. F. H. M., Kruize, Y. C. M., Schmitz, M., Kurt-Jones, E. A., Espevik, T., de Jong, E. C., Kapsenberg, M. L., Golenbock, D. T., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m206941200 · Cited by 587
Activation of Toll-Like Receptor-2 by Glycosylphosphatidylinositol Anchors from a Protozoan Parasite
Campos, M. A. S., Almeida, I. C., Takeuchi, O., Akira, S., Valente, E. P., Procópio, D. O., Travassos, L. R., Smith, J. A., Golenbock, D. T., & Gazzinelli, R. T. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.1.416 · Cited by 587
Interaction of Mycoplasmas With Host Cells
Rottem, S. (2003) · Physiological Reviews · doi:10.1152/physrev.00030.2002 · Cited by 432
Levy, O., Zarember, K. A., Roy, R. M., Cywes, C., Godowski, P. J., & Wessels, M. R. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.7.4627 · Cited by 375
Metadata
- Authors
- Mühlradt, P. F., Kieß, M., Meyer, H., Süßmuth, R., & Jung, G.
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 1997-06-02
- Volume
- 185
- Issue
- 11
- Pages
- 1951-1958
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2109148136
- Cited by
- 396
- References
- 50
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Mühlradt, P. F., Kieß, M., Meyer, H., Süßmuth, R., & Jung, G. (1997). Isolation, Structure Elucidation, and Synthesis of a Macrophage Stimulatory Lipopeptide from Mycoplasma fermentans Acting at Picomolar Concentration. The Journal of Experimental Medicine. https://doi.org/10.1084/jem.185.11.1951
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