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The Toll-like Receptor Protein Rp105 Regulates Lipopolysaccharide Signaling in B Cells
Abstract
The susceptibility to infections induced by Gram-negative bacteria is largely determined by innate immune responses to bacteria cell wall lipopolysaccharide (LPS). The stimulation of B cells by LPS enhances their antigen-presenting capacity and is accompanied by B cell proliferation and secretion of large quantities of LPS-neutralizing antibodies. Similar to macrophages and neutrophils, the LPS-induced activation of B cells is dependent on Toll-like receptor (TLR)4. Here, we demonstrate that the responses of B cells to LPS are also regulated by another TLR protein, RP105, which is predominantly expressed on mature B cells in mice and humans. The analysis of mice homozygous for the null mutation in the RP105 gene revealed impaired proliferative and humoral immune responses of RP105-deficient B cells to LPS. Using originally LPS-unresponsive Ba/F3 cells expressing exogenous TLR4 and RP105, we demonstrate the functional cooperation between TLR4 and RP105 in LPS-induced nuclear factor κB activation. These data suggest the existence of the TLR4–RP105 signaling module in the LPS-induced B cell activation.
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CD180 is related to TLRs, contains leucine-rich repeats, and binds Ly86 (MD1).
“CD180 is related to TLRs, contains leucine-rich repeats32,33 and binds Ly86 (MD1).34”
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Janeway, C. A., & Medzhitov, R. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.083001.084359 · Cited by 8,394
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Weekes, M. P., Antrobus, R., Talbot, S., Hör, S., Simecek, N., Smith, D. L., Bloor, S., Randow, F., & Lehner, P. J. (2012) · Journal of Proteome Research · doi:10.1021/pr201135e · Cited by 82
Metadata
- Authors
- Ogata, H., Su, I.-H., Miyake, K., Nagai, Y., Akashi, S., Mecklenbräuker, I., Rajewsky, K., Kimoto, M., & Tarakhovsky, A.
- Affiliations
- aDepartment of Immunology, Saga Medical School, Saga 849-8501, Japan
- bLaboratory of Lymphocyte Signaling, Institute for Genetics, University of Cologne, 50931 Cologne, Germany
- cDepartment of Immunology, Institute for Genetics, University of Cologne, 50931 Cologne, Germany
- Venue
- The Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2000-06-26
- Volume
- 192
- Issue
- 1
- Pages
- 23-30
- ISSN
- 0022-1007
- 1540-9538
- PMC
- PMC1887709
- Cited by
- 230
- References
- 0
- Language
- en
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