CoChat
Sign Up
Cited by 1,964Immune Response and InflammationImmunotherapy and Immune ResponsesImmune responses and vaccinations

The DOI matches its Crossref record; 43 of the 45 references it cites were found in a registry or an index.

Subsets of Human Dendritic Cell Precursors Express Different Toll-like Receptors and Respond to Different Microbial Antigens

Open access
Kadowaki, Norimitsu, Ho, Stephen, Antonenko, Svetlana, de Waal Malefyt, Rene, Kastelein, Robert A., Bazan, Fernando, Liu, Yong-Jun·2001·The Journal of Experimental Medicine
DOI 10.1084/jem.194.6.863PMC PMC2195968
Open this paper in CoChat — free accountOpen PDF

Abstract

Toll-like receptors (TLRs) are ancient microbial pattern recognition receptors highly conserved from Drosophila to humans. To investigate if subsets of human dendritic cell precursors (pre-DC), including monocytes (pre-DC1), plasmacytoid DC precursors (pre-DC2), and CD11c+ immature DCs (imDCs) are developed to recognize different microbes or microbial antigens, we studied their TLR expression and responses to microbial antigens. We demonstrate that whereas monocytes preferentially express TLR 1, 2, 4, 5, and 8, plasmacytoid pre-DC strongly express TLR 7 and 9. In accordance with these TLR expression profiles, monocytes respond to the known microbial ligands for TLR2 (peptidoglycan [PGN], lipoteichoic acid) and TLR4 (lipopolysaccharide), by producing tumor necrosis factor (TNF)-α and interleukin (IL)-6. In contrast, plasmacytoid pre-DCs only respond to the microbial TLR9-ligand, CpG-ODNs (oligodeoxynucleotides [ODNs] containing unmethylated CpG motifs), by producing IFN-α. CD11c+ imDCs preferentially express TLR 1, 2, and 3 and respond to TLR 2-ligand PGN by producing large amounts of TNF-α, and to viral double-stranded RNA-like molecule poly I:C, by producing IFN-α and IL-12. The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1084/jem.194.6.863

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 2 Quantitation of TLR mRNA by real-time quantitative RT-PCR in freshly isolated monocytes (Mono), CD11c+ imDCs, and plasmacytoid pre-DCs (pDC), and in immature and mature DCs induced from them. Monocytes were cultured with 50 ng/ml GM-CSF and 200 U/ml IL-4 for 5 d to obtain imDC1 and were further stimulated with CD40L-transfected L cells for 24 h to obtain mDC1. CD11c+ imDCs were stimulated with CD40L-transfected L cells for 24 h to obtain mature CD11c+ DCs. Plasmacytoid pre-DCs were cultured with 10 ng/ml IL-3 for 5 d to obtain imDC2 and were further stimulated with CD40L-transfected L cells for 24 h to obtain mature DC2. The amounts of mRNA were quantitated by real-time quantitative RT-PCR, and were shown by arbitrary unit relative to the amount of ubiquitin mRNA. The data shown are representative of three experiments.
Figure 2 Quantitation of TLR mRNA by real-time quantitative RT-PCR in freshly isolated monocytes (Mono), CD11c+ imDCs, and plasmacytoid pre-DCs (pDC), and in immature and mature DCs induced from them. Monocytes were cultured with 50 ng/ml GM-CSF and 200 U/ml IL-4 for 5 d to obtain imDC1 and were further stimulated with CD40L-transfected L cells for 24 h to obtain mDC1. CD11c+ imDCs were stimulated with CD40L-transfected L cells for 24 h to obtain mature CD11c+ DCs. Plasmacytoid pre-DCs were cultured with 10 ng/ml IL-3 for 5 d to obtain imDC2 and were further stimulated with CD40L-transfected L cells for 24 h to obtain mature DC2. The amounts of mRNA were quantitated by real-time quantitative RT-PCR, and were shown by arbitrary unit relative to the amount of ubiquitin mRNA. The data shown are representative of three experiments.CC BY-NC-SA · PubMed Central
Figure 1 Detection of TLR mRNA by RT-PCR in freshly isolated monocytes, CD11c+ imDCs, and plasmacytoid pre-DCs. The three cell populations were isolated by cell sorting to the purity of >99%. cDNAs were amplified for 35 cycles and were separated on a 3% agarose gel containing ethidium bromide. The data shown are representative of three experiments.
Figure 1 Detection of TLR mRNA by RT-PCR in freshly isolated monocytes, CD11c+ imDCs, and plasmacytoid pre-DCs. The three cell populations were isolated by cell sorting to the purity of >99%. cDNAs were amplified for 35 cycles and were separated on a 3% agarose gel containing ethidium bromide. The data shown are representative of three experiments.CC BY-NC-SA · PubMed Central
Figure 3 TNF-α, IL-6, IFN-α, and IL-12 production by monocytes (Mono), CD11c+ imDCs, and plasmacytoid pre-DCs (pDC) stimulated with microbial molecules that trigger different TLR signaling. The three cell populations were cultured with 10 μg/ml PGN, 10 μg/ml LTA, 10 μg/ml LPS, 50 μg/ml poly I:C, or with 5 μM CpG-ODN AAC-30 for 24 h, and the concentrations of cytokines in the supernatants were measured by ELISA. The data shown are representative of four or five experiments.
Figure 3 TNF-α, IL-6, IFN-α, and IL-12 production by monocytes (Mono), CD11c+ imDCs, and plasmacytoid pre-DCs (pDC) stimulated with microbial molecules that trigger different TLR signaling. The three cell populations were cultured with 10 μg/ml PGN, 10 μg/ml LTA, 10 μg/ml LPS, 50 μg/ml poly I:C, or with 5 μM CpG-ODN AAC-30 for 24 h, and the concentrations of cytokines in the supernatants were measured by ELISA. The data shown are representative of four or five experiments.CC BY-NC-SA · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 45 references

Cited by

Sorted by citations

Items 301–400 of 1,001

Related

See all

Metadata

Authors
Kadowaki, N., Ho, S., Antonenko, S., de Waal Malefyt, R., Kastelein, R. A., Bazan, F., & Liu, Y.-J.
Affiliations
aDNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304
ORCID
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
2001-09-17
Volume
194
Issue
6
Pages
863-870
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.194.6.863
PMC
PMC2195968
OpenAlex
W2104904748
Cited by
1,964
References
45
Citations by year
2026: 10 · 2025: 12 · 2024: 16 · 2023: 16 · 2022: 27 · 2021: 37 · 2020: 39 · 2019: 38 · 2018: 28 · 2017: 62 · 2016: 52 · 2015: 56 · 2014: 86 · 2013: 90 · 2012: 95
FWCI
40.14
Type
journal article
Language
en
Open access
Locations
  • The Journal of Experimental Medicine (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
Keywords
Toll-like receptors, dendritic cell precursors, plasmacytoid dendritic cells, monocytes, TLR2, TLR4, TLR7, TLR9, peptidoglycan, lipoteichoic acid, lipopolysaccharide, CpG oligodeoxynucleotides, poly(I:C), TNF-α, interleukin-6, interferon-alpha, IL-12, dendritic cell development
MeSH terms
Dendritic Cells, Hematopoietic Stem Cells, Humans, Lipopolysaccharides, Membrane Glycoproteins, Monocytes, Oligodeoxyribonucleotides, Receptors, Cell Surface, Staphylococcus aureus, Biomarkers, Gene Expression, Cytokines, Drosophila Proteins, Toll-Like Receptors, Toll-Like Receptor 1, Toll-Like Receptor 2, Toll-Like Receptor 4, Toll-Like Receptor 7, Toll-Like Receptor 9

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review