Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function

Haines, Christopher J.; Giffon, Thierry D.; Lu, Li-Sheng; Lu, Xiaowei; Tessier-Lavigne, Marc; Ross, Douglas T.; Lewis, David B.

doi:10.1084/jem.20080996

Cited by 189T-cell and B-cell ImmunologyPsoriasis: Treatment and PathogenesisImmunotherapy and Immune Responses

Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function

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Haines, Christopher J., Giffon, Thierry D., Lu, Li-Sheng, Lu, Xiaowei, Tessier-Lavigne, Marc, Ross, Douglas T., Lewis, David B.·2009·Journal of Experimental Medicine

DOI 10.1084/jem.20080996PMC PMC2646563

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Abstract

CD4+ recent thymic emigrants (RTEs) comprise a clinically and immunologically important T cell population that indicates thymic output and that is essential for maintaining a diverse αβ–T cell receptor (TCR) repertoire of the naive CD4+ T cell compartment. However, their frequency and function are poorly understood because no known surface markers distinguish them from older non-RTE naive CD4+ T cells. We demonstrate that protein tyrosine kinase 7 (PTK7) is a novel marker for human CD4+ RTEs. Consistent with their recent thymic origin, human PTK7+ RTEs contained higher levels of signal joint TCR gene excision circles and were more responsive to interleukin (IL)-7 compared with PTK7− naive CD4+ T cells, and rapidly decreased after complete thymectomy. Importantly, CD4+ RTEs proliferated less and produced less IL-2 and interferon-γ than PTK7− naive CD4+ T cells after αβ-TCR/CD3 and CD28 engagement. This immaturity in CD4+ RTE effector function may contribute to the reduced CD4+ T cell immunity observed in contexts in which CD4+ RTEs predominate, such as in the fetus and neonate or after immune reconstitution. The ability to identify viable CD4+ RTEs by PTK7 staining should be useful for monitoring thymic output in both healthy individuals and in patients with genetic or acquired CD4+ T cell immunodeficiencies.

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Protein tyrosine kinase 7 and CD31 are expressed on a subpopulation of naive cells in children and young adults, are lost with homeostatic proliferation, and decline with age.
“The cell surface molecules protein tyrosine kinase 7 and CD31, expressed on a subpopulation of naive cells in children and young adults, are lost with homeostatic proliferation and decline with age”
From Successful and Maladaptive T Cell Aging (2017) · cites this paper as Haines et al., 2009

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Authors: Haines, C. J., Giffon, T. D., Lu, L.-S., Lu, X., Tessier-Lavigne, M., Ross, D. T., & Lewis, D. B.
Affiliations: Howard Hughes Medical Institute (US)
Department of Pediatrics and the Immunology Program 1 , 2 , and 3
Corresponding author: Lewis, David B.
ORCID: Giffon, Thierry D.
Lu, Xiaowei
Ross, Douglas T.
Lewis, David B.
Venue: Journal of Experimental Medicine
Publisher: Rockefeller University Press
Published: 2009-01-26
Volume: 206
Issue: 2
Pages: 275-285
ISSN: 0022-1007 (ISSN-L)
1540-9538
DOI: 10.1084/jem.20080996
PMC: PMC2646563
OpenAlex: W2112729981
Cited by: 189
References: 30
Citations by year: 2026: 5 · 2025: 7 · 2024: 9 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 8 · 2019: 7 · 2018: 22 · 2017: 10 · 2016: 10 · 2015: 14 · 2014: 9 · 2013: 22 · 2012: 17
FWCI: 6.61
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: cc-by-nc-sa
Locations: The Journal of Experimental Medicine (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
PubMed Central (publishedVersion, cc-by-nc-sa)
PubMed (publishedVersion)
Funders: National Institute of Allergy and Infectious Diseases (T32AI007290)
Topics: Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords: recent thymic emigrants, CD4+ T cells, PTK7, naive T cells, thymic output, IL-7, T cell proliferation, interleukin-2, interferon-gamma, thymectomy, T cell deficiency, immune reconstitution
MeSH terms: Age Factors, Animals, Cell Movement, Cricetulus, Flow Cytometry, Cricetinae, Humans, Immunity, Cellular, Thymus Gland, Biomarkers, CD4-Positive T-Lymphocytes, Cell Adhesion Molecules, Immunophenotyping, CHO Cells, DNA Primers, Receptor Protein-Tyrosine Kinases

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Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function

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