A reliable and simplified sj/β-TREC ratio quantification method for human thymic output measurement
Abstract
Current techniques to peripherally assess thymic function are: the signal-joint T-cell receptor excision circle (sj-TREC) level measurement and the naive T cell and CD31+ TREC-rich subset determination. However, all of them are indirect approaches and none could be considered a direct recent thymic emigrant (RTE) marker. To overcome their limitations, Dion et al. (2004) described the sj/beta-TREC ratio that allows the peripheral quantification of the double negative to double positive intrathymic proliferation step. Nevertheless, the protocol described is expensive, sample and time-consuming, thus, limiting its usefulness. In this study, we describe a simplified protocol that reduces from 33 to 9 the amount of PCR reaction needed but maintaining the sensitivity and reproducibility of the original technique. In addition, we corroborated the effectiveness of our technique as an accurate thymic output-related marker by correlating the peripheral sj/beta-TREC ratio with a direct measurement of thymic function as the percentage of double positive thymocytes (r=0.601, p<0.001).
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Thymic output persists, albeit reduced, up until around 70 years of age.
“While studies continue to support the age-associated reduction in thymic function, evidence now points towards a more gradual decline, whereby thymic output persists, albeit reduced, up until around 70 years of age (Douek et al. 1998; Ferrando-Martinez et al. 2010).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Ferrando-Martinez et al. 2010
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- Authors
- Ferrando-Martínez, S., Franco, J. M., Ruiz-Mateos, E., Hernández, A., Ordoñez, A., Gutierrez, E., & Leal, M.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Hospital Universitario Virgen del Rocío (ES)
- Instituto de Biomedicina de Sevilla (ES)
- Corresponding author
- Leal, Manuel
- Venue
- Journal of Immunological Methods
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 352
- Issue
- 1-2
- Pages
- 111-117
- ISSN
- 0022-1759 (ISSN-L)
- OpenAlex
- W2094247899
- Cited by
- 59
- References
- 35
- Citations by year
- 2026: 4 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 5 · 2016: 10 · 2015: 2 · 2014: 5 · 2013: 2 · 2012: 11
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Immunological Methods (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Instituto de Salud Carlos III (RECAVA RD06/0014, RETIC RD06/0006/0021)
- Fundación Para La Innovación Y La Prospectiva En Salud En España (12481/05, 36624/06)
- Servicio Andaluz de Salud (156/2006, PI0366/07)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunodeficiency and Autoimmune Disorders
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- thymic output, recent thymic emigrants, TREC, thymic function, double-positive thymocytes, naive T cells
- MeSH terms
- Antigens, Differentiation, Cell Differentiation, Female, Fetal Blood, Humans, Receptors, Antigen, T-Cell, Thymus Gland, Reproducibility of Results, Gene Rearrangement, Antigens, CD, Polymerase Chain Reaction, Cell Proliferation
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