Cited by 208Antimicrobial Resistance in Staphylococcusvaccines and immunoinformatics approachesBiochemical and Structural Characterization

Identification of In Vivo–Expressed Antigens of Staphylococcus aureus and Their Use in Vaccinations for Protection against Nasal Carriage

Clarke, Simon R., Brummell, Kirsten J., Horsburgh, Malcolm J., McDowell, Philip W., Mohamad, Sharifah A. Syed, Stapleton, Melanie R., Acevedo, Jorge, Read, Robert C. +5 more·2006·The Journal of Infectious Diseases

Abstract

A spectrum of in vivo-expressed Staphylococcus aureus antigens was identified by probing bacteriophage expression libraries of S. aureus with serum samples from infected and uninfected individuals. Eleven recombinant antigenic proteins were produced, and specific antibody titers in a large collection of human serum samples were determined. Significantly increased concentrations of reactive immunoglobulin G (IgG) to 7 antigens were found in serum samples from ill individuals, compared with those in healthy individuals. Significantly higher concentrations of reactive IgG to 4 antigens, including iron-responsive surface determinant (Isd) A and IsdH, were found in serum samples from healthy individuals who were not nasal carriers of S. aureus, compared with those in healthy carriers. Vaccination of cotton rats with IsdA or IsdH protected against nasal carriage. Also, IsdA is involved in adherence of S. aureus to human desquamated nasal epithelial cells and is required for nasal colonization in the cotton rat model. Thus, vaccination with these antigens may prevent S. aureus carriage and reduce the prevalence of human disease.

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  • IsdA facilitates binding to human desquamated epithelial cells and promotes nasal colonization in rats.

    “Consistent with this hypothesis, IsdA facilitates binding to human desquamated epithelial cells, and promotes nasal colonization in rats [122, 126, 127].”
  • IsdA and IsdB were identified as vaccine candidates using reverse vaccinology, and immunization with these proteins is protective in murine models of infection.

    “IsdA and IsdB were identified as vaccine candidates using “reverse vaccinology” [119], and subsequent studies confirmed that immunization with these proteins is protective in murine models of infection [117, 120–123].”

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Research works printed but not linked: 4

  1. Marchese A, Schito GC, Debbia EA. Emergence of drug-resistant gram- positive pathogenic bacteria. J Chemother 2000; 12:459-62.
  2. Bredius RGM, de Vries CE, Troelstra A, et al. Phagocytosis of Staph- ylococcus aureus and Haemophilus influenzae type B opsonized with polyclonal human IgG1 and IgG2 antibodies. J Immunol 1993; 151: 1463-72.
  3. Kauppi M, Saarinen L, Ka ¨yhty H. Anti-capsular polysaccharide anti- bodies reduce nasopharyngeal colonization by Haemophilus influenzae type b in infant rats. J Infect Dis 1993; 167:365-71.
  4. Andrews SC, Robinson AK, Rodrı ´guez-Quin ˜ones F. Bacterial iron ho- meostasis. FEMS Microbiol Rev 2003; 27:215-37.

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Authors
Clarke, S. R., Brummell, K. J., Horsburgh, M. J., McDowell, P. W., Mohamad, S. A. S., Stapleton, M. R., Acevedo, J., Read, R. C., Day, N. P. J., Peacock, S. J., Mond, J. J., Kokai‐Kun, J. F., & Foster, S. J.
Affiliations
  • University of Sheffield (GB)
  • Mahidol University (TH)
Venue
The Journal of Infectious Diseases
Published
2006-04-15
Volume
193
Issue
8
Pages
1098-1108
ISSN
  • 0022-1899 (ISSN-L)
  • 1537-6613
OpenAlex
W2103675658
Cited by
208
References
49
Citations by year
2026: 2 · 2025: 4 · 2024: 7 · 2023: 4 · 2022: 4 · 2021: 1 · 2020: 6 · 2019: 9 · 2018: 10 · 2017: 8 · 2016: 12 · 2015: 19 · 2014: 8 · 2013: 12 · 2012: 16
FWCI
9.50
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Infectious Diseases (publishedVersion)
  • PubMed (publishedVersion)
  • ePrints Soton (University of Southampton) (submittedVersion)
Funders
  • Vanderbilt University
  • Wellcome Trust
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
Staphylococcus aureus, nasal carriage, bacterial antigens, IgG, ISDA, IsdH, vaccine development, bacterial adhesion, cotton rat model, antibody titers
MeSH terms
Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Vaccines, Carrier State, Cells, Cultured, Epithelial Cells, Female, Humans, Immunoglobulin G, Nasal Cavity, Receptors, Cell Surface, Recombinant Proteins, Staphylococcal Infections, Staphylococcus aureus, Sigmodontinae, DNA Primers, Cation Transport Proteins

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