The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Perfusion Improves Tissue Architecture of Engineered Cardiac Muscle
Abstract
Cardiac muscle with a certain threshold thickness, uniformity of tissue architecture, and functionality would expand the therapeutic options currently available to patients with congenital or acquired cardiac defects. Cardiac constructs cultured in well-mixed medium had an approximately 100-microm-thick peripheral tissue-like region around a relatively cell-free interior, a structure consistent with the presence of concentration gradients within the tissue. We hypothesized that direct perfusion of cultured constructs can reduce diffusional distances for mass transport, improve control of oxygen, pH, nutrients and metabolites in the cell microenvironment, and thereby increase the thickness and spatial uniformity of engineered cardiac muscle. To test this hypothesis, constructs (9.5-mm-diameter, 2-mm-thick discs) based on neonatal rat cardiac myocytes and fibrous polyglycolic acid scaffolds were cultured either directly perfused with medium or in control spinner flasks. Perfusion improved the spatial uniformity of cell distribution and enhanced the expression of cardiac-specific markers, presumably due to the improved control of local microenvironmental conditions within the forming tissue. Medium perfusion could thus be utilized to better mimic the transport conditions within native cardiac muscle and enable in vitro engineering of cardiac constructs with clinically useful thicknesses.
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Cites
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Self-citationRegenerating functional myocardium: Improved performance after skeletal myoblast transplantation
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Self-citationThe permeability of fibrous porous media
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Cited by
Challenges in Cardiac Tissue Engineering
Vunjak-Novakovic, G., Tandon, N., Godier, A., Maidhof, R., Marsano, A., Martens, T. P., & Radisic, M. (2010) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2009.0352 · Cited by 511
Self-citationEngineered Microenvironments for Controlled Stem Cell Differentiation
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Self-citationOxygen gradients correlate with cell density and cell viability in engineered cardiac tissue
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Self-citationMedium perfusion enables engineering of compact and contractile cardiac tissue
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Metadata
- Authors
- Carrier, R. L., Rupnick, M., Langer, R., Schoen, F. J., Freed, L. E., & Vunjak-Novakovic, G.
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2002-04
- Volume
- 8
- Issue
- 2
- Pages
- 175-188
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W2099635527
- Cited by
- 323
- References
- 42
- FWCI
- 12.70
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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