Blueberry ( Vaccinium virgatum ) Leaf Extracts Protect Against Aβ-Induced Cytotoxicity and Cognitive Impairment
Abstract
The ethylacetate (EtOAc) fraction of blueberry leaf extract was investigated to examine the in vivo antiamnesic effects against amyloid β protein (Aβ)-induced learning and memory deficit. The fraction showed the highest antioxidant activities, and the generation of intracellular reactive oxygen species was significantly decreased. Cell viability assays revealed the in vitro cytoprotective effects of the fraction, and the cytoplasmic lactate dehydrogenase release into the medium was dose-dependently inhibited. In addition, a chlorogenic acid was identified as a predominant phenolic compound by high-performance liquid chromatography analysis. Antiamnesic effects were evaluated by using in vivo the Y-maze and passive avoidance tests, and preadministration of the fraction attenuated Aβ-induced memory impairment in both in vivo experiments. Acetylcholinesterase prepared from mice brain was inhibited by the fraction, and malondialdehyde generation in the brain homogenate was also decreased. These findings suggest that the EtOAc fraction of blueberry leaf extract could possess a wide range of physiological effects against neurodegenerative diseases.
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Blueberry leaf extract (ethyl acetate fraction, 5-20 mg/kg) alleviated amyloid beta-induced memory impairment by inhibiting AChE activity and oxidative stress in amyloid beta-treated mice.
“Blueberry leaf extract (ethyl acetate fraction, 5–20 mg/kg) alleviated Aβ-induced memory impairment by inhibiting AChE activity and oxidative stress in Aβ-treated mice [81].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [81]
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- Authors
- Jeong, H. R., Jo, Y. N., Jeong, J. H., Kim, H. J., Kim, M.-J., & Heo, H. J.
- Affiliations
- Gyeongsang National University (KR)
- Department of Special Purpose Trees, Korea Forest Research Institute, Suwon, Korea.
- Venue
- Journal of Medicinal Food
- Publisher
- SAGE Publications
- Published
- 2013-11
- Volume
- 16
- Issue
- 11
- Pages
- 968-976
- ISSN
- 1096-620X (ISSN-L)
- 1557-7600
- OpenAlex
- W1973073191
- Cited by
- 26
- References
- 42
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 4 · 2021: 2 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 3
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medicinal Food (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- Vaccinium virgatum, amyloid-beta, Alzheimer's disease, anti-amnesic effect, learning and memory impairment, chlorogenic acid, antioxidant activity, reactive oxygen species, acetylcholinesterase inhibition, neuroprotection, neurodegenerative diseases, ethyl acetate fraction
- MeSH terms
- Animals, Antioxidants, Cell Survival, Chlorogenic Acid, Cholinesterase Inhibitors, Cognition, Cognition Disorders, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, Male, Malondialdehyde, Phytotherapy, Memory Disorders, Mice, Inbred ICR, Neurons, Phenols, Plant Extracts, Amyloid beta-Peptides, PC12 Cells, Reactive Oxygen Species, Oxidative Stress, Plant Leaves, Blueberry Plants, Mice, Rats
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