The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Human Bone Marrow and Adipose Tissue Mesenchymal Stem Cells: A User's Guide
Open accessAbstract
Mesenchymal stem cells (MSCs) are adult stem cells that hold great promise in the field of regenerative medicine. They can be isolated from almost any tissue of the body and display, after expansion, very similar properties and minor differences, probably due to their microenvironment of origin. Expansion in vitro can be obtained in cytokine-free, serum-enriched media, as well as in serum-free, basic fibroblast growth factor-enriched media. A detailed immunophenotypic analysis is required to test the purity of the preparation, but no unique distinguishing marker has been described as yet. Functional assays, that is, differentiation studies in vitro, are needed to prove multilineage differentiation of expanded cells, and demonstration of pluripotency is necessary to identify most immature precursors. MSCs show powerful immunomodulative properties toward most of the cells of the immune system: this strengthens the theoretical rationale for their use also in an allogeneic setting across the major histocompatibility complex (MHC) immunological barriers. Systemic intravenous injection and local use have been tried: after systemic injection, MSCs show a high degree of chemotaxis based on pro-inflammatory cytokines, and localize at inflamed and neoplastic tissues; local regeneration has been improved using synthetic, as well as organic scaffolds. On the other hand, inadequate heterotopic in vivo differentiation and neoplastic transformation are potential risks of this form of cell therapy, even if evidence of this sort has been collected only from studies in mice, and generally after prolonged in vitro expansion. This review tries to provide a detailed technical overview of the methods used for human bone-marrow (BM)-derived and adipose-tissue (AT)-derived MSC isolation, in vitro expansion, and characterization for tissue repair. We chose to use BM-MSCs as a model to describe techniques that have been used for MSC isolation and expansion from very different sources, and AT-MSCs as an example of a reliable and increasingly common alternative source.
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Cites
Multilineage potential of adult human mesenchymal stem cells
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., & Marshak, D. R. (1999) · Science · doi:10.1126/science.284.5411.143 · Cited by 21,052
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Human mesenchymal stem cells modulate allogeneic immune cell responses
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A perivascular origin for mesenchymal stem cells in multiple human organs
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“Even if MSCs are expandable from virtually all tissues [10], to date the preferred source remains the BM.”
Kern, S., Eichler, H., Stoeve, J., Klüter, H., & Bieback, K. (2006) · STEM CELLS · doi:10.1634/stemcells.2005-0342 · Cited by 3,370
Isolation of Mesenchymal Stem Cells of Fetal or Maternal Origin from Human Placenta
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Stem-cell ageing modified by the cyclin-dependent kinase inhibitor p16INK4a
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Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma
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Adult human mesenchymal stem cell as a target for neoplastic transformation
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Earlier Onset of Syngeneic Tumors in the Presence of Mesenchymal Stem Cells
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Cited by
Li, C.-Y., Wu, X.-Y., Tong, J.-B., Yang, X.-X., Zhao, J.-L., Zheng, Q.-F., Zhao, G.-B., & Ma, Z.-J. (2015) · Stem Cell Research & Therapy · doi:10.1186/s13287-015-0066-5 · Cited by 410
LI, X., BAI, J., JI, X., LI, R., XUAN, Y., & WANG, Y. (2014) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2014.1821 · Cited by 301
Mesenchymal stem cells: Amazing remedies for bone and cartilage defects
Kangari, P., Talaei-Khozani, T., Razeghian-Jahromi, I., & Razmkhah, M. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-02001-1 · Cited by 286
The secretome of periodontal ligament stem cells from MS patients protects against EAE
Rajan, T. S., Giacoppo, S., Diomede, F., Ballerini, P., Paolantonio, M., Marchisio, M., Piattelli, A., Bramanti, P., Mazzon, E., & Trubiani, O. (2016) · Scientific Reports · doi:10.1038/srep38743 · Cited by 109
Duma, C., Kopyov, O., Kopyov, A., Berman, M., Lander, E., Elam, M., Arata, M., Weiland, D., Cannell, R., Caraway, C., et al. (2019) · Molecular Biology Reports · doi:10.1007/s11033-019-04983-5 · Cited by 85
Metadata
- Authors
- Mosna, F., Sensebé, L., & Krampera, M.
- Venue
- Stem Cells and Development
- Publisher
- SAGE Publications
- Published
- 2010-10
- Volume
- 19
- Issue
- 10
- Pages
- 1449-1470
- ISSN
- 1525-8165 (ISSN-L)
- 1547-3287
- 1557-8534
- OpenAlex
- W2120840499
- Cited by
- 330
- References
- 10
- FWCI
- 12.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Mosna, F., Sensebé, L., & Krampera, M. (2010). Human Bone Marrow and Adipose Tissue Mesenchymal Stem Cells: A User's Guide. Stem Cells and Development. https://doi.org/10.1089/scd.2010.0140
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
