The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Tissue-Engineered Follicles Produce Live, Fertile Offspring
Open accessAbstract
Oocytes grown in vitro are of low quality and yield few live births, thus limiting the ability to store or bank the ova of women wishing to preserve their fertility. We applied tissue engineering principles to the culture of immature mouse follicles by designing an alginate hydrogel matrix to maintain the oocyte's 3- dimensional (3D) architecture and cell-cell interactions in vitro. A 3D culture mimics the in vivo follicle environment, and hydrogel-encapsulated follicles develop mature oocytes within the capacity for fertilization similar to that of oocytes matured in vivo. Embryos derived from cultured oocytes fertilized in vitro and transferred to pseudopregnant female mice were viable, and both male and female offspring were fertile. Our results demonstrate that alginate hydrogel-based 3D in vitro culture of follicles permits normal growth and development of follicles and oocytes. This system creates new opportunities for discovery in follicle biology and establishes a core technology for human egg banks for preservation of fertility.
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Cites
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Livebirth after orthotopic transplantation of cryopreserved ovarian tissue
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Alginate type and RGD density control myoblast phenotype
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Cited by
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047
3D bioprinting of tissues and organs for regenerative medicine
Vijayavenkataraman, S., Yan, W.-C., Lu, W. F., Wang, C.-H., & Fuh, J. Y. H. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.07.004 · Cited by 630
The ovary: basic biology and clinical implications
Richards, J. S., & Pangas, S. A. (2010) · Journal of Clinical Investigation · doi:10.1172/jci41350 · Cited by 561
Laronda, M. M., Rutz, A. L., Xiao, S., Whelan, K. A., Duncan, F. E., Roth, E. W., Woodruff, T. K., & Shah, R. N. (2017) · Nature Communications · doi:10.1038/ncomms15261 · Cited by 527
Self-citationPhysical properties of alginate hydrogels and their effects on in vitro follicle development
West, E. R., Xu, M., Woodruff, T. K., & Shea, L. D. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.07.001 · Cited by 371
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Metadata
- Authors
- Xu, M., Kreeger, P. K., Shea, L. D., & Woodruff, T. K.
- Venue
- Tissue Engineering
- Publisher
- SAGE Publications
- Published
- 2006-10
- Volume
- 12
- Issue
- 10
- Pages
- 2739-2746
- ISSN
- 1076-3279 (ISSN-L)
- 1557-8690
- OpenAlex
- W2091960270
- Cited by
- 386
- References
- 25
- FWCI
- 15.54
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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