The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Effects of Dispensing Pressure and Nozzle Diameter on Cell Survival from Solid Freeform Fabrication–Based Direct Cell Writing
Abstract
Novel technologies are emerging that incorporate cells as part of the building blocks for various biomanufacturing processes, such as solid freeform fabricated tissue constructs for tissue regeneration, three-dimensional pharmacokinetic models, cell-based microelectromechanical systems, sensors, and microfluidic devices. However, the effects of these biomanufacturing processes on cells have not been fully studied. This paper examines the effect of solid freeform fabrication-based direct cell writing process, focusing on dispensing pressure and nozzle size, on the viability and functional behavior of HepG2 cells encapsulated within alginate. Our experimental results revealed a process-induced mechanical damage to cell membrane integrity, causing a quantifiable loss in cell viability due to incremental increases and decreases in the studied process parameters of dispensing pressure and nozzle size, respectively. The experimental results also suggested that cells may require a recovery period following direct cell writing biofabrication. The general finding of this study may be applicable to freeform fabrication of cell-based tissue constructs and three-dimensional biological models.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Integrins and Signal Transduction Pathways: the Road Taken
Clark, E. A., & Brugge, J. S. (1995) · Science · doi:10.1126/science.7716514 · Cited by 2,937
TENSEGRITY: THE ARCHITECTURAL BASIS OF CELLULAR MECHANOTRANSDUCTION
Ingber, D. E. (1997) · Annual Review of Physiology · doi:10.1146/annurev.physiol.59.1.575 · Cited by 1,566
Patterning proteins and cells using soft lithography
Kane, R. (1999) · Biomaterials · doi:10.1016/s0142-9612(99)00165-9 · Cited by 1,490
Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton
Ingber, D. E. (1993) · Journal of Cell Science · doi:10.1242/jcs.104.3.613 · Cited by 1,155
Inkjet printing of viable mammalian cells
Xu, T., Jin, J., Gregory, C., Hickman, J. J., & Boland, T. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.04.011 · Cited by 1,035
Apparent viscosity and cortical tension of blood granulocytes determined by micropipet aspiration
Evans, E., & Yeung, A. (1989) · Biophysical Journal · doi:10.1016/s0006-3495(89)82660-8 · Cited by 788
Mechanical behavior in living cells consistent with the tensegrity model
Wang, N., Naruse, K., Stamenović, D., Fredberg, J. J., Mijailovich, S. M., Tolić-Nørrelykke, I. M., Polte, T., Mannix, R., & Ingber, D. E. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.141199598 · Cited by 741
Viability and electrophysiology of neural cell structures generated by the inkjet printing method
Xu, T., Gregory, C. A., Molnar, P., Cui, X., Jalota, S., Bhaduri, S. B., & Boland, T. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.01.048 · Cited by 520
Plopper, G. E., McNamee, H. P., Dike, L. E., Bojanowski, K., & Ingber, D. E. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.10.1349 · Cited by 504
Microfluidics technology for manipulation and analysis of biological cells
Yi, C., Li, C.-W., Ji, S., & Yang, M. (2006) · Analytica Chimica Acta · doi:10.1016/j.aca.2005.12.037 · Cited by 478
Cited by
Additive manufacturing (3D printing): A review of materials, methods, applications and challenges
Ngo, T. D., Kashani, A., Imbalzano, G., Nguyen, K. T. Q., & Hui, D. (2018) · Composites Part B: Engineering · doi:10.1016/j.compositesb.2018.02.012 · Cited by 8,431
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034
3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
Kolesky, D. B., Truby, R. L., Gladman, A. S., Busbee, T. A., Homan, K. A., & Lewis, J. A. (2014) · Advanced Materials · doi:10.1002/adma.201305506 · Cited by 2,102
Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink
Pati, F., Jang, J., Ha, D.-H., Won Kim, S., Rhie, J.-W., Shim, J.-H., Kim, D.-H., & Cho, D.-W. (2014) · Nature Communications · doi:10.1038/ncomms4935 · Cited by 1,904
Progress in 3D bioprinting technology for tissue/organ regenerative engineering
Matai, I., Kaur, G., Seyedsalehi, A., McClinton, A., & Laurencin, C. T. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119536 · Cited by 1,156
Metadata
- Authors
- Chang, R., Nam, J., & Sun, W.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2008-01
- Volume
- 14
- Issue
- 1
- Pages
- 41-48
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W2016058943
- Cited by
- 549
- References
- 35
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Effects of Dispensing Pressure and Nozzle Diameter on Cell Survival from Solid Freeform Fabrication–Based Direct Cell Writing. https://app.cochat.ai/papers/10.1089/ten.a.2007.0004
- The paper
- Chang, R., Nam, J., & Sun, W. (2008). Effects of Dispensing Pressure and Nozzle Diameter on Cell Survival from Solid Freeform Fabrication–Based Direct Cell Writing. Tissue Engineering Part A. https://doi.org/10.1089/ten.a.2007.0004
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
