The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Development of a Decellularized Lung Bioreactor System for Bioengineering the Lung: The Matrix Reloaded
Open accessAbstract
We developed a decellularized murine lung matrix bioreactor system that could be used to evaluate the potential of stem cells to regenerate lung tissue. Lungs from 2-3-month-old C57BL/6 female mice were excised en bloc with the trachea and heart, and decellularized with sequential solutions of distilled water, detergents, NaCl, and porcine pancreatic DNase. The remaining matrix was cannulated and suspended in small airway growth medium, attached to a ventilator to simulate normal, murine breathing-induced stretch. After 7 days in an incubator, lung matrices were analyzed histologically. Scanning electron microscopy and histochemical staining demonstrated that the pulmonary matrix was intact and that the geographic placement of the proximal and distal airways, alveoli and vessels, and the basement membrane of these structures all remained intact. Decellularization was confirmed by the absence of nuclear 4',6-diamidino-2-phenylindole staining and negative polymerase chain reaction for genomic DNA. Collagen content was maintained at normal levels. Elastin, laminin, and glycosaminglycans were also present, although at lower levels compared to nondecellularized lungs. The decellularized lung matrix bioreactor was capable of supporting growth of fetal alveolar type II cells. Analysis of day 7 cryosections of fetal-cell-injected lung matrices showed pro-Sp-C, cytokeratin 18, and 4',6-diamidino-2-phenylindole-positive cells lining alveolar areas that appeared to be attached to the matrix. These data illustrate the potential of using decellularized lungs as a natural three-dimensional bioengineering matrix as well as provide a model for the study of lung regeneration from pulmonary stem cells.
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Cites
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Decellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222
Extracellular matrix as a biological scaffold material: Structure and function
BADYLAK, S., FREYTES, D., & GILBERT, T. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.013 · Cited by 1,671
Oligosaccharides of Hyaluronan Activate Dendritic Cells via Toll-like Receptor 4
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Reprint of: Extracellular matrix as a biological scaffold material: Structure and function
Badylak, S. F., Freytes, D. O., & Gilbert, T. W. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.07.016 · Cited by 1,362
Allometric Relationships of Cell Numbers and Size in the Mammalian Lung
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Quantification of DNA in Biologic Scaffold Materials
Gilbert, T. W., Freund, J. M., & Badylak, S. F. (2009) · Journal of Surgical Research · doi:10.1016/j.jss.2008.02.013 · Cited by 481
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The Basement Membrane Component of Biologic Scaffolds Derived from Extracellular Matrix
Brown, B., Lindberg, K., Reing, J., Stolz, D. B., & Badylak, S. F. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.519 · Cited by 436
Cited by
An overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
25th Anniversary Article: Rational Design and Applications of Hydrogels in Regenerative Medicine
Annabi, N., Tamayol, A., Uquillas, J. A., Akbari, M., Bertassoni, L. E., Cha, C., Camci‐Unal, G., Dokmeci, M. R., Peppas, N. A., & Khademhosseini, A. (2014) · Advanced Materials · doi:10.1002/adma.201303233 · Cited by 1,409
Badylak, S. F., Taylor, D., & Uygun, K. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124743 · Cited by 1,041
Hogan, B. L. M., Barkauskas, C. E., Chapman, H. A., Epstein, J. A., Jain, R., Hsia, C. C. W., Niklason, L., Calle, E., Le, A., Randell, S. H., et al. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.07.012 · Cited by 876
Tissue Engineering and Regenerative Medicine: History, Progress, and Challenges
Berthiaume, F., Maguire, T. J., & Yarmush, M. L. (2011) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-061010-114257 · Cited by 734
Metadata
- Authors
- Price, A. P., England, K. A., Matson, A. M., Blazar, B. R., & Panoskaltsis-Mortari, A.
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2010-08
- Volume
- 16
- Issue
- 8
- Pages
- 2581-2591
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- OpenAlex
- W2130806934
- Cited by
- 344
- References
- 45
- FWCI
- 25.15
- Type
- journal article
- Language
- en
- Open access
- green
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- Price, A. P., England, K. A., Matson, A. M., Blazar, B. R., & Panoskaltsis-Mortari, A. (2010). Development of a Decellularized Lung Bioreactor System for Bioengineering the Lung: The Matrix Reloaded. Tissue Engineering Part A. https://doi.org/10.1089/ten.tea.2009.0659
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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