Cited by 35Tissue Engineering and Regenerative MedicineCollagen: Extraction and CharacterizationXenotransplantation and immune response

Absence of Immune Responses with Xenogeneic Collagen and Elastin

Open access
Bayrak, Alexandra, Prüger, Pauline, Stock, Ulrich A., Seifert, Martina·2013·Tissue Engineering Part A

Abstract

Novel tissue-engineering approaches for cardiovascular matrices based on xenogeneic extracellular matrix protein (ECMp) constituents require a detailed evaluation of their interaction with essential immune cell subsets playing a role in innate or adaptive immunity. Therefore, in this study, the effects of xenogeneic (porcine, bovine) collagen type I and elastin as the two main components of the heart valve ECM were analyzed in comparison to their human equivalents. First, their potential to induce maturation and cytokine secretion of human dendritic cells (DC) was tested by flow cytometry. Second, the influence on proliferation and cytokine release of purified human B and T cells was measured. We could demonstrate that xenogeneic collagen type I and elastin are not able to trigger the maturation of DC as verified by the lack of CD83 induction accompanied by a low tumor necrosis factor-α release. Moreover, both ECMp showed no effect on the proliferation and the interleukin-6 release of either unstimulated or prestimulated B cells. Additionally, anti-CD3-induced purified T cell proliferation and secretion of cytokines was not affected. All in vitro data verify the low immunogenicity of porcine and bovine collagen type I and elastin and favor their suitability for tissue-engineered scaffolds.

Integrity signals

Registry

Crossref · 10.1089/ten.tea.2012.0394

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Interspecies variations between ECM components such as collagen, laminin, fibronectin, elastin, and growth factors are not extreme, making xenogeneic ECM plausible surrogates for their human-derived counterparts.

    “Interspecies variations between ECM components such as collagen, laminin, fibronectin, elastin, and growth factors are not extreme, making xenogeneic ECM plausible surrogates for their human-derived counterparts [114, 117–119].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Bayrak, A., Prüger, P., Stock, U. A., & Seifert, M.
Affiliations
  • Berlin-Brandenburger Centrum für Regenerative Therapien (DE)
  • University Hospital Frankfurt (DE)
Corresponding author
Seifert, Martina
Venue
Tissue Engineering Part A
Published
2013-04-11
Volume
19
Issue
13-14
Pages
1592-1600
ISSN
  • 1937-3341 (ISSN-L)
  • 1937-335X
OpenAlex
W2062678179
Cited by
35
References
54
Citations by year
2026: 4 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 3 · 2013: 2
FWCI
2.30
Type
journal article
Language
en
Open access
Locations
  • Tissue Engineering Part A (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • Bundesministerium für Bildung und Forschung
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
Keywords
elastin, extracellular matrix proteins, immunogenicity, dendritic cell maturation, CD83, cytokine production, T cell proliferation, B cell proliferation, interleukin-6, TNF-α, heart valve tissue engineering, tissue engineering scaffolds
MeSH terms
Animals, Cattle, Collagen, Dendritic Cells, Elastin, Extracellular Matrix, Humans, Leukocytes, Mononuclear, Swine, Tumor Necrosis Factor-alpha

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review