Absence of Immune Responses with Xenogeneic Collagen and Elastin
Open accessAbstract
Novel tissue-engineering approaches for cardiovascular matrices based on xenogeneic extracellular matrix protein (ECMp) constituents require a detailed evaluation of their interaction with essential immune cell subsets playing a role in innate or adaptive immunity. Therefore, in this study, the effects of xenogeneic (porcine, bovine) collagen type I and elastin as the two main components of the heart valve ECM were analyzed in comparison to their human equivalents. First, their potential to induce maturation and cytokine secretion of human dendritic cells (DC) was tested by flow cytometry. Second, the influence on proliferation and cytokine release of purified human B and T cells was measured. We could demonstrate that xenogeneic collagen type I and elastin are not able to trigger the maturation of DC as verified by the lack of CD83 induction accompanied by a low tumor necrosis factor-α release. Moreover, both ECMp showed no effect on the proliferation and the interleukin-6 release of either unstimulated or prestimulated B cells. Additionally, anti-CD3-induced purified T cell proliferation and secretion of cytokines was not affected. All in vitro data verify the low immunogenicity of porcine and bovine collagen type I and elastin and favor their suitability for tissue-engineered scaffolds.
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Interspecies variations between ECM components such as collagen, laminin, fibronectin, elastin, and growth factors are not extreme, making xenogeneic ECM plausible surrogates for their human-derived counterparts.
“Interspecies variations between ECM components such as collagen, laminin, fibronectin, elastin, and growth factors are not extreme, making xenogeneic ECM plausible surrogates for their human-derived counterparts [114, 117–119].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [118]
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- Authors
- Bayrak, A., Prüger, P., Stock, U. A., & Seifert, M.
- Affiliations
- Berlin-Brandenburger Centrum für Regenerative Therapien (DE)
- University Hospital Frankfurt (DE)
- Corresponding author
- Seifert, Martina
- Venue
- Tissue Engineering Part A
- Publisher
- SAGE Publications
- Published
- 2013-04-11
- Volume
- 19
- Issue
- 13-14
- Pages
- 1592-1600
- ISSN
- 1937-3341 (ISSN-L)
- 1937-335X
- PMC
- PMC3665304
- OpenAlex
- W2062678179
- Cited by
- 35
- References
- 54
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 3 · 2013: 2
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part A (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Bundesministerium für Bildung und Forschung
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Keywords
- elastin, extracellular matrix proteins, immunogenicity, dendritic cell maturation, CD83, cytokine production, T cell proliferation, B cell proliferation, interleukin-6, TNF-α, heart valve tissue engineering, tissue engineering scaffolds
- MeSH terms
- Animals, Cattle, Collagen, Dendritic Cells, Elastin, Extracellular Matrix, Humans, Leukocytes, Mononuclear, Swine, Tumor Necrosis Factor-alpha
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