Novel Utilization of Serum in Tissue Decellularization
Open accessAbstract
Decellularization of native tissues is a promising technique with numerous applications in tissue engineering and regenerative medicine. However, there are various limitations of currently available decellularization methods, such as alteration of extracellular matrix mechanics and restricted use on certain tissues. This study was conducted to explore the effect of serum on the decellularization of various types of tissues. Fetal bovine serum-containing cell culture medium endothelial growth media-2 removed DNA but not cellular beta-actin from human umbilical artery after detergent treatment, without compromising the tissue mechanical strength assessed by burst pressure. In addition, the effect of serum-containing endothelial growth media-2 on DNA removal was replicated in other types of tissues such as tissue-engineered vessels and myocardium. Other types of serum, including human serum, were also shown to remove DNA from detergent-pretreated tissues. In conclusion, we describe a novel utilization of serum that may have broad applications in tissue decellularization.
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Enzyme residues may negatively affect the recellularization process or simulate an adverse immune response.
“Furthermore, enzyme residues may negatively affect the recellularization process or simulate an adverse immune response [147,148].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [147]
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“Furthermore, enzyme residues may negatively affect the recellularization process or simulate an adverse immune response [147,148].”
Background
- Authors
- Gui, L., Chan, S. A., Breuer, C. K., & Niklason, L. E.
- Affiliations
- Yale University (US)
- Corresponding author
- Niklason, Laura E.
- Venue
- Tissue Engineering Part C: Methods
- Publisher
- SAGE Publications
- Published
- 2010-04
- Volume
- 16
- Issue
- 2
- Pages
- 173-184
- ISSN
- 1937-3384 (ISSN-L)
- 1937-3392
- PMC
- PMC2946886
- OpenAlex
- W2068782007
- Cited by
- 109
- References
- 4
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 1 · 2021: 9 · 2020: 11 · 2019: 11 · 2018: 7 · 2017: 8 · 2016: 8 · 2015: 11 · 2014: 7 · 2013: 9 · 2012: 9
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part C Methods (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (NIH-R01, 5r01hl083895-09)
- National Heart, Lung, and Blood Institute (R01HL-083895)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- serum, fetal bovine serum, DNA removal, detergent treatment, human umbilical artery, myocardium, extracellular matrix mechanics, burst pressure, human serum
- MeSH terms
- Animals, Biomechanical Phenomena, Blood Vessels, Cattle, Cell Fractionation, Cells, Cultured, Culture Media, Detergents, DNA, Heart, Humans, Materials Testing, Rats, Inbred F344, Stress, Mechanical, Swine, Umbilical Arteries, Tissue Engineering, Serum, Rats
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