Influence of pH on Extracellular Matrix Preservation During Lung Decellularization
Open accessAbstract
The creation of decellularized organs for use in regenerative medicine requires the preservation of the organ extracellular matrix (ECM) as a means to provide critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. The purpose of this study was to assess the influence of varying pH levels on the preservation of key ECM components during the decellularization of rat lungs. Herein, we show that the pH of the 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)-based decellularization solution influences ECM retention, cell removal, and also the potential for host response upon implantation of acellular lung tissue. The preservation of ECM components, including elastin, fibronectin, and laminin, were better retained in the lower pH conditions that were tested (pH ranges tested: 8, 10, 12); glycosaminoglycans were preserved to a higher extent in the lower pH groups as well. The DNA content following decellularization of the rat lung was inversely correlated with the pH of the decellularization solution. Despite detectible levels of cyotoskeletal proteins and significant residual DNA, tissues decellularized at pH 8 demonstrated the greatest tissue architecture maintenance and the least induction of host response of all acellular conditions. These results highlight the effect of pH on the results obtained by organ decellularization and suggest that altering the pH of the solutions used for decellularization may influence the ability of cells to properly differentiate and home to appropriate locations within the scaffold, based on the preservation of key ECM components and implantation results.
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More aggressive protocols designed for decellularization at higher pH diminish fibronectin, laminin, elastin, and GAGs' content and integrity, which interferes with subsequent cell adhesion and proliferation.
“Notably, more aggressive protocols designed for decellularization in higher pH diminish fibronectin, laminin, elastin, and GAGs’ content and integrity, which in turn interfere with subsequent cell adhesion and proliferation [82].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [82]
Higher pH shatters ECM and produces particles that elicit more severe immune reactions.
“Presumably, higher pH shatters ECM and produces particles that elicit more severe immune reactions [81, 82].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [82]
Alkalines such as NaOH separate DNA strands at pH>11 and extract cell components, but decellularization at higher pH provokes a more robust immune response than in neutral conditions.
“Alkalines such as NaOH separate DNA strands at pH>11 and extract cell components, but decellularization at higher pH provokes a more robust immune response than in neutral conditions.”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [82]
CHAPS deplete elastin and GAGs content of ECM.
“Of note, they have been demonstrated to deplete the elastin and GAGs content of ECM [51, 75, 82].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [82]
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- Authors
- Tsuchiya, T., Balestrini, J. L., Mendez, J., Calle, E. A., Zhao, L., & Niklason, L. E.
- Affiliations
- Yale University (US)
- Corresponding author
- Niklason, Laura E.
- Venue
- Tissue Engineering Part C: Methods
- Publisher
- SAGE Publications
- Published
- 2014-12
- Volume
- 20
- Issue
- 12
- Pages
- 1028-1036
- ISSN
- 1937-3384 (ISSN-L)
- 1937-3392
- PMC
- PMC4241865
- OpenAlex
- W2084112522
- Cited by
- 89
- References
- 34
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 10 · 2023: 8 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 5 · 2018: 5 · 2017: 7 · 2016: 6 · 2015: 15 · 2014: 2
- FWCI
- 9.92
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Tissue Engineering Part C Methods (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Nagasaki University's Academic Output SITE (Nagasaki University) (submittedVersion, cc-by-nc-nd)
- Funders
- National Heart, Lung, and Blood Institute (R01HL098220, U01HL111016)
- National Institute of General Medical Sciences (T32GM086287)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- extracellular matrix preservation, pH, CHAPS, elastin, fibronectin, laminin, glycosaminoglycans, residual DNA, host response, tissue structure, rat lung, regenerative medicine
- MeSH terms
- Animals, Cholic Acids, Collagen, Elastin, Extracellular Matrix, Fibronectins, Glycosaminoglycans, Hydrogen-Ion Concentration, Laminin, Lung, Male, Rats, Sprague-Dawley, Implants, Experimental, Tissue Engineering, Subcutaneous Tissue
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