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Cited by 607

The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.

Receptor-mediated Endocytosis in the Caenorhabditis elegans Oocyte

Open access
Grant, Barth, Hirsh, David·1999·Molecular Biology of the Cell

Abstract

The Caenorhabditis elegans oocyte is a highly amenable system for forward and reverse genetic analysis of receptor-mediated endocytosis. We describe the use of transgenic strains expressing a vitellogenin::green fluorescent protein (YP170::GFP) fusion to monitor yolk endocytosis by theC. elegans oocyte in vivo. This YP170::GFP reporter was used to assay the functions of C. eleganspredicted proteins homologous to vertebrate endocytosis factors using RNA-mediated interference. We show that the basic components and pathways of endocytic trafficking are conserved between C. elegans and vertebrates, and that this system can be used to test the endocytic functions of any new gene. We also used the YP170::GFP assay to identify rme(receptor-mediated endocytosis) mutants. We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor.

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Metadata

Authors
Grant, B., & Hirsh, D.
Venue
Molecular Biology of the Cell
Published
1999-12
Volume
10
Issue
12
Pages
4311-4326
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2159803165
Cited by
607
References
71
FWCI
4.56
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Receptor-mediated Endocytosis in the Caenorhabditis elegans Oocyte. https://app.cochat.ai/papers/10.1091/mbc.10.12.4311
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Grant, B., & Hirsh, D. (1999). Receptor-mediated Endocytosis in the Caenorhabditis elegans Oocyte. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.10.12.4311

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