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The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes
Open accessAbstract
Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all membranes known to communicate with early endosomes, including the plasma membrane, clathrin-coated pits, late endosomes, and membranes of thetrans-Golgi network. Affinity-purified antibodies that block the ability of endobrevin/VAMP-8 to form SNARE core complexes potently inhibit homotypic fusion of both early and late endosomes in vitro. Fab fragments were as active as intact immunoglobulin Gs. Recombinant endobrevin/VAMP-8 inhibited both fusion reactions with similar potency. We conclude that endobrevin/VAMP-8 operates as an R-SNARE in the homotypic fusion of early and late endosomes.
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“This complex brings endosomes/phagosomes in close proximity as a prerequisite for fusion (probably involving VAMP7 and 8) (35, 36).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [36]
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Cites
Greene, L., & Tischler, A. S. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.7.2424 · Cited by 5,566
Wessel, D., & Flügge, U. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90782-6 · Cited by 3,997
SNAP receptors implicated in vesicle targeting and fusion
Söllner, T. H., Whiteheart, S. W., Brunner, M., Erdjument‐Bromage, H., Geromanos, S. J., Tempst, P., & Rothman, J. E. (1993) · Nature · doi:10.1038/362318a0 · Cited by 3,239
Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
Sutton, R. B., Fasshauer, D., Jahn, R., & Brunger, A. T. (1998) · Nature · doi:10.1038/26412 · Cited by 2,417 · cited 1×
“In the neuronal SNARE complex, three Q-SNARE motifs (one contributed by syntaxin and two by SNAP-25) and one R-SNARE motif (contributed by synaptobrevin/VAMP) form an extended helical bundle (Sutton et al., 1998).”
Self-citationMechanisms of intracellular protein transport
Rothman, J. E. (1994) · Nature · doi:10.1038/372055a0 · Cited by 2,267
Wiedenmann, B. J., & Franke, W. W. (1985) · Cell · doi:10.1016/s0092-8674(85)80082-9 · Cited by 1,432
A new method of preparing gold probes for multiple-labeling cytochemistry.
Slot, J. W., & Geuze, H. J. (1985) · PubMed · Cited by 1,403
Membrane fusion and exocytosis.
Jahn, R., & Südhof, T. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.863 · Cited by 1,253
Self-citationEEA1 links PI(3)K function to Rab5 regulation of endosome fusion
Simonsen, A., Lippe, R., Christoforidis, S., Gaullier, J.-M., Brech, A., Callaghan, J., Toh, B.-H., Murphy, C., Zerial, M., & Stenmark, H. (1998) · Nature · doi:10.1038/28879 · Cited by 1,133
rab5 controls early endosome fusion in vitro
Gorvel, J., Chavrier, P., Zerial, M., & Gruenberg, J. E. (1991) · Cell · doi:10.1016/0092-8674(91)90316-q · Cited by 1,094
A TECHNIQUE FOR ULTRACRYOTOMY OF CELL SUSPENSIONS AND TISSUES
Tokuyasu, K. T. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.57.2.551 · Cited by 1,080
Fasshauer, D., Sutton, R. B., Brünger, A. T., & Jahn, R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.26.15781 · Cited by 1,055
Self-citationA 38,000-dalton membrane protein (p38) present in synaptic vesicles.
Jahn, R., SCHIEBLER, W., Ouimet, C. C., & Greengard, P. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.12.4137 · Cited by 851
Self-citationSynaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.
Hayashi, T., McMahon, H. T., Yamasaki, S., Binz, T., Hata, Y., Südhof, T. C., & Niemann, H. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06834.x · Cited by 798
The diversity of Rab proteins in vesicle transport
Novick, P., & Zerial, M. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80025-7 · Cited by 757
Navone, F., Jahn, R., Gioia, G. D., Stukenbrok, H., Greengard, P., & Camilli, P. V. D. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.103.6.2511 · Cited by 674
Self-citationGörlich, D. (1993) · Cell · doi:10.1016/0092-8674(93)90483-7 · Cited by 658
Rab 7: an important regulator of late endocytic membrane traffic.
Feng, Y., Press, B., & Wandinger‐Ness, A. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.6.1435 · Cited by 637
Horiuchi, H., Lippé, R., McBride, H. M., Rubino, M., Woodman, P. G., Stenmark, H., Rybin, V., Wilm, M. S., Ashman, K., Mann, M., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80380-3 · Cited by 595
Gruenberg, J. E., Griffiths, G. W., & Howell, K. E. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.4.1301 · Cited by 586
Improving structural integrity of cryosections for immunogold labeling
Liou, W., Geuze, H. J., & Slot, J. W. (1996) · Histochemistry and Cell Biology · doi:10.1007/bf02473201 · Cited by 521
McBride, H. M., Rybin, V., Murphy, C., Giner, A., Teasdale, R., & Zerial, M. (1999) · Cell · doi:10.1016/s0092-8674(00)81966-2 · Cited by 520
McMahon, H. T., Ushkaryov, Y. A., Edelmann, L., Link, E., Binz, T., Niemann, H., Jahn, R., & Südhof, T. C. (1993) · Nature · doi:10.1038/364346a0 · Cited by 509
Self-citationRabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion
Stenmark, H., Vitale, G., Ullrich, O., & Zerial, M. (1995) · Cell · doi:10.1016/0092-8674(95)90120-5 · Cited by 474
Homotypic vacuolar fusion mediated by t- and v-SNAREs
Nichols, B. J., Ungermann, C., Pelham, H. R. B., Wickner, W. T., & Haas, A. (1997) · Nature · doi:10.1038/387199a0 · Cited by 455
Görlich, D., Prehn, S., Hartmann, E., Kalies, K., & Rapoport, T. A. (1992) · Cell · doi:10.1016/0092-8674(92)90517-g · Cited by 447
Aniento, F. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.123.6.1373 · Cited by 404
Neurotransmitter release — four years of SNARE complexes
Hanson, P. I., Heuser, J. E., & Jahn, R. (1997) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(97)80057-8 · Cited by 394
Self-citationSynaptic transmission: A bidirectional and self-modifiable form of cell-cell communication
Jessell, T. M., & Kandel, E. R. (1993) · Cell · doi:10.1016/s0092-8674(05)80025-x · Cited by 373
Ravichandran, V., Chawla, A., & Roche, P. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.23.13300 · Cited by 354
A marker of early amacrine cell development in rat retina
Barnstable, C. J., Hofstein, R., & Akagawa, K. (1985) · Developmental Brain Research · doi:10.1016/0165-3806(85)90116-6 · Cited by 322
Mixed and Non-cognate SNARE Complexes
Fasshauer, D., Antonin, W., Margittai, M., Pabst, S., & Jahn, R. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.22.15440 · Cited by 316
Self-citationSNARE Interactions Are Not Selective
Yang, B., Gonzalez, L. C., Prekeris, R., Steegmaier, M., Advani, R. J., & Scheller, R. H. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.9.5649 · Cited by 315
Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate
Burger, P. M., Mehl, E., Cameron, P. L., Maycox, P. R., Baumert, M., Lottspeich, F., Camilli, P. V. D., & Jahn, R. (1989) · Neuron · doi:10.1016/0896-6273(89)90240-7 · Cited by 299
Self-citationFasshauer, D., Eliason, W. K., Brünger, A. T., & Jahn, R. (1998) · Biochemistry · doi:10.1021/bi980542h · Cited by 270
Self-citationSeven Novel Mammalian SNARE Proteins Localize to Distinct Membrane Compartments
Advani, R. J., Bae, H.-R., Bock, J. B., Chao, D. S., Doung, Y.-C., Prekeris, R., Yoo, J.-S., & Scheller, R. H. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.17.10317 · Cited by 263
Rabouille, C., Kondo, H., Newman, R., Hui, N., Freemont, P. S., & Warren, G. (1998) · Cell · doi:10.1016/s0092-8674(00)81128-9 · Cited by 256
Mannose 6-phosphate receptors in sorting and transport of lysosomal enzymes
Hille-Rehfeld, A. (1995) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(95)00004-b · Cited by 249
Galli, T., Chilcote, T. J., Mundigl, O., Binz, T., Niemann, H., & Camilli, P. V. D. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.5.1015 · Cited by 226
Barriocanal, J. G., Bonifacino, J. S., Yuan, L. C., & Sandoval, I. V. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66630-x · Cited by 217
Three Novel Proteins of the Syntaxin/SNAP-25 Family
Steegmaier, M., Yang, B., Yoo, J.-S., Huang, B. C. B., Shen, M., Yu, S., Luo, Y., & Scheller, R. H. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.51.34171 · Cited by 206
Vamp-7 Mediates Vesicular Transport from Endosomes to Lysosomes
Advani, R. J., Yang, B., Prekeris, R., Lee, K. C., Klumperman, J., & Scheller, R. H. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.146.4.765 · Cited by 204
Export of Cellubrevin from the Endoplasmic Reticulum Is Controlled by BAP31
Annaert, W. G., Becker, B., Kistner, U., Reth, M. G., & Jahn, R. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.139.6.1397 · Cited by 169
Self-citationA model for structural similarity between different SNARE complexes based on sequence relationships
Weimbs, T., Mostov, K. E., Low, S. H., & Hofmann, K. (1998) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01285-9 · Cited by 153
Syntaxin 5 regulates endoplasmic reticulum to Golgi transport.
Dascher, C., Matteson, J., & Balch, W. E. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)43884-7 · Cited by 151
Vesicle-associated Membrane Protein 4 is Implicated inTrans-Golgi Network Vesicle Trafficking
Steegmaier, M., Klumperman, J., Foletti, D. L., Yoo, J.-S., & Scheller, R. H. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.6.1957 · Cited by 150
Reconstitution of Retrograde Transport from the Golgi to the ER In Vitro
Spang, A., & Schekman, R. W. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.3.589 · Cited by 140
von Mollard, G. F., Stahl, B., Walch-Solimena, C., Takei, K., Daniels, L. S., Khoklatchev, A., Camilli, P. V. D., Südhof, T. C., & Jahn, R. (1994) · PubMed · Cited by 127
Self-citationUngermann, C., & Wickner, W. T. (1998) · The EMBO Journal · doi:10.1093/emboj/17.12.3269 · Cited by 118
Wong, S. H., Zhang, T., Xu, Y., Subramaniam, V. N., Griffiths, G. W., & Hong, W. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.6.1549 · Cited by 115
Bock, J. B., & Scheller, R. H. (1997) · Nature · doi:10.1038/387133a0 · Cited by 98
Götte, M. (1998) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01272-0 · Cited by 92
Syntaxin 12, a Member of the Syntaxin Family Localized to the Endosome
Tang, B. L., Tan, A. E. H., Lim, L. K., Lee, S. S., Low, D. Y. H., & Hong, W. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.12.6944 · Cited by 91
SCAMP 37, a new marker within the general cell surface recycling system.
Brand, S., & Castle, J. D. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06053.x · Cited by 86
Syntaxin 7, a Novel Syntaxin Member Associated with the Early Endosomal Compartment
Wong, S. H., Xu, Y., Zhang, T., & Hong, W. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.375 · Cited by 86
Fusion of Endosomes Involved in Synaptic Vesicle Recycling
Holroyd, C., Kistner, U., Annaert, W. G., & Jahn, R. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.9.3035 · Cited by 86
Self-citationMammalian Sly1 Regulates Syntaxin 5 Function in Endoplasmic Reticulum to Golgi Transport
Dascher, C., & Balch, W. E. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.27.15866 · Cited by 84
GS32, a Novel Golgi SNARE of 32 kDa, Interacts Preferentially with Syntaxin 6
Wong, S. H., Xu, Y., Zhang, T., Griffiths, G. W., Lowe, S. L., Subramaniam, V. N., Seow, K. T., & Hong, W. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.1.119 · Cited by 73
Cleavage of cellubrevin by tetanus toxin does not affect fusion of early endosomes.
Link, E., McMahon, H. T., von Mollard, G. F., Yamasaki, S., Niemann, H., Südhof, T. C., & Jahn, R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)46640-3 · Cited by 66
Self-citationNSF is required for transport from early to late endosomes
Robinson, L. J., Aniento, F., & Grüenberg, J. (1997) · Journal of Cell Science · doi:10.1242/jcs.110.17.2079 · Cited by 44
Research works printed but not linked: 2
- Fischer von Mollard G., 1994, Eur. J. Cell Biol., 65, 319
- Slot J.W., 1985, Eur. J. Cell Biol., 38, 87
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Weiss, G., & Schaible, U. E. (2015) · Immunological Reviews · doi:10.1111/imr.12266 · Cited by 989
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Antonin, W., Holroyd, C., Fasshauer, D., Pabst, S., von Mollard, G. F., & Jahn, R. (2000) · The EMBO Journal · doi:10.1093/emboj/19.23.6453 · Cited by 290
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Metadata
- Authors
- Antonin, W., Holroyd, C., Tikkanen, R., Höning, S., & Jahn, R.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- University of Bonn (DE)
- University of Göttingen (DE)
- Venue
- Molecular Biology of the Cell
- Published
- 2000-10
- Volume
- 11
- Issue
- 10
- Pages
- 3289-3298
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC14992
- OpenAlex
- W2148157392
- Cited by
- 156
- References
- 60
- Citations by year
- 2026: 3 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 1 · 2018: 6 · 2017: 10 · 2016: 5 · 2015: 8 · 2014: 6 · 2013: 5 · 2012: 14
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- National Science Foundation
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- R-SNARE, homotypic fusion, early endosomes, late endosomes, membrane trafficking, clathrin-coated pits, trans-Golgi network, endosomal membrane fusion
- MeSH terms
- Animals, Cell Fractionation, Cell Membrane, Cell-Free System, Coated Pits, Cell-Membrane, Membrane Fusion, Membrane Proteins, Endosomes, Synaptophysin, trans-Golgi Network, Vesicular Transport Proteins, SNARE Proteins, R-SNARE Proteins, Qa-SNARE Proteins, Rats
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