The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes
Open accessAbstract
Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates. Using subcellular fractionation and quantitative immunogold electron microscopy, we found that endobrevin/VAMP-8 is present on all membranes known to communicate with early endosomes, including the plasma membrane, clathrin-coated pits, late endosomes, and membranes of thetrans-Golgi network. Affinity-purified antibodies that block the ability of endobrevin/VAMP-8 to form SNARE core complexes potently inhibit homotypic fusion of both early and late endosomes in vitro. Fab fragments were as active as intact immunoglobulin Gs. Recombinant endobrevin/VAMP-8 inhibited both fusion reactions with similar potency. We conclude that endobrevin/VAMP-8 operates as an R-SNARE in the homotypic fusion of early and late endosomes.
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“This complex brings endosomes/phagosomes in close proximity as a prerequisite for fusion (probably involving VAMP7 and 8) (35, 36).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [36]
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SNAREs — engines for membrane fusion
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Metadata
- Authors
- Antonin, W., Holroyd, C., Tikkanen, R., Höning, S., & Jahn, R.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- University of Bonn (DE)
- University of Göttingen (DE)
- Venue
- Molecular Biology of the Cell
- Published
- 2000-10
- Volume
- 11
- Issue
- 10
- Pages
- 3289-3298
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- PMC
- PMC14992
- OpenAlex
- W2148157392
- Cited by
- 156
- References
- 60
- Citations by year
- 2026: 3 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 1 · 2018: 6 · 2017: 10 · 2016: 5 · 2015: 8 · 2014: 6 · 2013: 5 · 2012: 14
- FWCI
- 1.92
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Molecular Biology of the Cell (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- National Science Foundation
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- R-SNARE, homotypic fusion, early endosomes, late endosomes, membrane trafficking, clathrin-coated pits, trans-Golgi network, endosomal membrane fusion
- MeSH terms
- Animals, Cell Fractionation, Cell Membrane, Cell-Free System, Coated Pits, Cell-Membrane, Membrane Fusion, Membrane Proteins, Endosomes, Synaptophysin, trans-Golgi Network, Vesicular Transport Proteins, SNARE Proteins, R-SNARE Proteins, Qa-SNARE Proteins, Rats
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