The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
RNAi-mediated Hip1R Silencing Results in Stable Association between the Endocytic Machinery and the Actin Assembly Machinery
Open accessAbstract
Actin filaments transiently associate with the endocytic machinery during clathrin-coated vesicle formation. Although several proteins that might mediate or regulate this association have been identified, in vivo demonstration of such an activity has not been achieved. Huntingtin interacting protein 1R (Hip1R) is a candidate cytoskeletal-endocytic linker or regulator because it binds to clathrin and actin. Here, Hip1R levels were lowered by RNA interference (RNAi). Surprisingly, rather than disrupting the transient association between endocytic and cytoskeletal proteins, clathrin-coated structures (CCSs) and their endocytic cargo became stably associated with dynamin, actin, the Arp2/3 complex, and its activator, cortactin. RNAi double-depletion experiments demonstrated that accumulation of the cortical actin-endocytic complexes depended on cortactin. Fluorescence recovery after photobleaching showed that dynamic actin filament assembly can occur at CCSs. Our results provide evidence that Hip1R helps to make the interaction between actin and the endocytic machinery functional and transient.
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Citations
Cites
Actin Assembly and Endocytosis: From Yeast to Mammals
Engqvist-Goldstein, Å. E. Y., & Drubin, D. G. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111401.093127 · Cited by 572
Self-citationActivation of Arp2/3 complex-mediated actin polymerization by cortactin
Uruno, T., Liu, J., Zhang, P., Fan, Y.-X., Egile, C., Li, R., Mueller, S. C., & Zhan, X. (2001) · Nature Cell Biology · doi:10.1038/35060051 · Cited by 568
Cited by
Mechanisms of clathrin-mediated endocytosis
Kaksonen, M., & Roux, A. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.132 · Cited by 1,698
Harnessing actin dynamics for clathrin-mediated endocytosis
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1940 · Cited by 706
Self-citationActin dynamics counteract membrane tension during clathrin-mediated endocytosis
Boulant, S., Kural, C., Zeeh, J.-C., Ubelmann, F., & Kirchhausen, T. (2011) · Nature Cell Biology · doi:10.1038/ncb2307 · Cited by 566
Turcot, V., Lu, Y., Highland, H. M., Schurmann, C., Justice, A. E., Fine, R. S., Bradfield, J. P., Esko, T., Giri, A., Graff, M., et al. (2018) · Nature Genetics · doi:10.1038/s41588-017-0011-x · Cited by 409
A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis
Yarar, D., Waterman-Storer, C. M., & Schmid, S. L. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-09-0774 · Cited by 408
Metadata
- Authors
- Engqvist-Goldstein, Å. E. Y., Zhang, C. X., Carreno, S., Barroso, C., Heuser, J. E., & Drubin, D. G.
- Venue
- Molecular Biology of the Cell
- Published
- 2004-04
- Volume
- 15
- Issue
- 4
- Pages
- 1666-1679
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2132009450
- Cited by
- 161
- References
- 2
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Engqvist-Goldstein, Å. E. Y., Zhang, C. X., Carreno, S., Barroso, C., Heuser, J. E., & Drubin, D. G. (2004). RNAi-mediated Hip1R Silencing Results in Stable Association between the Endocytic Machinery and the Actin Assembly Machinery. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e03-09-0639
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