The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells
Open accessAbstract
Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein with functions in euchromatin and heterochromatin. Here we investigated the diffusional behaviors of HP1 isoforms in mammalian cells. Using fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) we found that in interphase cells most HP1 molecules (50–80%) are highly mobile (recovery halftime: t 1/2≈ 0.9 s; diffusion coefficient: D ≈ 0.6–0.7 μm 2 s-1). Twenty to 40% of HP1 molecules appear to be incorporated into stable, slow-moving oligomeric complexes (t 1/2≈ 10 s), and constitutive heterochromatin of all mammalian cell types analyzed contain 5–7% of very slow HP1 molecules. The amount of very slow HP1 molecules correlated with the chromatin condensation state, mounting to more than 44% in condensed chromatin of transcriptionally silent cells. During mitosis 8–14% of GFP-HP1α, but not the other isoforms, are very slow within pericentromeric heterochromatin, indicating an isoform-specific function of HP1α in heterochromatin of mitotic chromosomes. These data suggest that mobile as well as very slow populations of HP1 may function in concert to maintain a stable conformation of constitutive heterochromatin throughout the cell cycle.
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Cites
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
Lachner, M., O'Carroll, D., Rea, S., Mechtler, K., & Jenuwein, T. (2001) · Nature · doi:10.1038/35065132 · Cited by 2,868
Regulation of chromatin structure by site-specific histone H3 methyltransferases
Rea, S., Eisenhaber, F., O'Carroll, D., Strahl, B. D., Sun, Z.-W., Schmid, M., Opravil, S., Mechtler, K., Ponting, C. P., Allis, C. D., et al. (2000) · Nature · doi:10.1038/35020506 · Cited by 2,845 · cited 1×
“The formation and spreading of heterochromatin is believed to be driven by oligomerization of HP1 on meK9-H3 modified nucleosomes concomitant with further recruitment of methyltransferases (Rea et al., 2000; Hall et al., 2002).”
BackgroundSchultz, D. C., Ayyanathan, K., Negorev, D., Maul, G. G., & Rauscher, F. J. (2002) · Genes & Development · doi:10.1101/gad.973302 · Cited by 1,313
Heterochromatin and Epigenetic Control of Gene Expression
Grewal, S. I. S., & Moazed, D. (2003) · Science · doi:10.1126/science.1086887 · Cited by 1,065
Kinetics of Core Histones in Living Human Cells
Kimura, H., & Cook, P. R. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.7.1341 · Cited by 675
Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin
Peters, A. H. F. M., Mermoud, J. E., O'Carroll, D., Pagani, M., Schweizer, D., Brockdorff, N., & Jenuwein, T. (2002) · Nature Genetics · doi:10.1038/ng789 · Cited by 502
Nielsen, A. L., Ortiz, J. A., You, J., Oulad‐Abdelghani, M., Khechumian, R., Gansmuller, A., Chambon, P., & Losson, R. (1999) · The EMBO Journal · doi:10.1093/emboj/18.22.6385 · Cited by 361
Heterochromatin Dynamics in Mouse Cells
Murzina, N., Verreault, A., Laue, E., & Stillman, B. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80204-x · Cited by 300
Cited by
Lund, A. H., & van Lohuizen, M. (2004) · Genes & Development · doi:10.1101/gad.1232504 · Cited by 489
DNA Binding Selectivity of MeCP2 Due to a Requirement for A/T Sequences Adjacent to Methyl-CpG
Klose, R. J., Sarraf, S. A., Schmiedeberg, L., McDermott, S. M., Stancheva, I., & Bird, A. P. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.07.021 · Cited by 344
Self-citationSpreading of silent chromatin: inaction at a distance
Talbert, P. B., & Henikoff, S. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1920 · Cited by 300
Heterochromatin protein 1 is recruited to various types of DNA damage
Luijsterburg, M. S., Dinant, C., Lans, H., Stap, J., Wiernasz, E., Lagerwerf, S., Warmerdam, D. O., Lindh, M., Brink, M. C., Dobrucki, J. W., et al. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200810035 · Cited by 264
Manukyan, M., & Singh, P. B. (2014) · Scientific Reports · doi:10.1038/srep04789 · Cited by 45
Metadata
- Authors
- Schmiedeberg, L., Weisshart, K., Diekmann, S., Meyer zu Hoerste, G., & Hemmerich, P.
- Venue
- Molecular Biology of the Cell
- Published
- 2004-06
- Volume
- 15
- Issue
- 6
- Pages
- 2819-2833
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2135318926
- Cited by
- 160
- References
- 8
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Schmiedeberg, L., Weisshart, K., Diekmann, S., Meyer zu Hoerste, G., & Hemmerich, P. (2004). High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e03-11-0827
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