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Skeletal Muscle Disuse Atrophy and the Rehabilitative Role of Protein in Recovery from Musculoskeletal Injury
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“As rehabilitation progresses and activity increases, protein is vital for rebuilding lean mass and enhancing overall recovery [68].”
From Youth and Adolescent Athlete Musculoskeletal Health: Dietary and Nutritional Strategies to Optimise Injury Prevention and Support Recovery (2024) · cites this paper as [68]
In the citing paper
“Furthermore, protein has been shown to play a role in preventing disuse atrophy by stimulating muscle protein synthesis (MPS) and reducing muscle protein breakdown (MPB) during periods of inactivity or immobilisation [68,69].”
From Youth and Adolescent Athlete Musculoskeletal Health: Dietary and Nutritional Strategies to Optimise Injury Prevention and Support Recovery (2024) · cites this paper as [68]
In the citing paper
“Distributing protein intake throughout the day (e.g., 0.3 g/kg BM across five meals and snacks) provides the necessary EAAs to minimise muscle loss and support healing [39,61,68].”
From Youth and Adolescent Athlete Musculoskeletal Health: Dietary and Nutritional Strategies to Optimise Injury Prevention and Support Recovery (2024) · cites this paper as [68]
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Citations
Cites
Identification of ubiquitin ligases required for skeletal muscle atrophy.
Bodine, S. C., Latres, E., Baumhueter, S., Lai, V. K.-M., Nunez, L., Clarke, B. A., Poueymirou, W. T., Panaro, F. J., Na, E., Dharmarajan, K., et al. (2001) · Science · doi:10.1126/science.1065874 · Cited by 3,521
Bauer, J., Biolo, G., Cederholm, T., Cesari, M., Cruz-Jentoft, A. J., Morley, J. E., Phillips, S., Sieber, C., Stehle, P., Teta, D., et al. (2013) · Journal of the American Medical Directors Association · doi:10.1016/j.jamda.2013.05.021 · Cited by 2,553 · cited 1×
“8 g · kg−1 · d−1 in the control group, however, which may be inadequate in older populations (71).”
BackgroundMultiple types of skeletal muscle atrophy involve a common program of changes in gene expression
Lecker, S. H., Jagoe, R. T., Gilbert, A., Gomes, M., Baracos, V., Bailey, J., Price, S. R., Mitch, W. E., & Goldberg, A. L. (2004) · The FASEB Journal · doi:10.1096/fj.03-0610com · Cited by 1,539 · cited 1×
“Transcript levels of MAFbx and MuRF1 are increased under several conditions of muscle atrophy (46).”
BackgroundSestrin2 is a leucine sensor for the mTORC1 pathway
Wolfson, R. L., Chantranupong, L., Saxton, R. A., Shen, K., Scaria, S. M., Cantor, J. R., & Sabatini, D. M. (2016) · Science · doi:10.1126/science.aab2674 · Cited by 1,306
Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle
Cuthbertson, D., Smith, K., Babraj, J., Leese, G., Waddell, T., Atherton, P., Wackerhage, H., Taylor, P. M., & Rennie, M. J. (2005) · The FASEB Journal · doi:10.1096/fj.04-2640fje · Cited by 1,211 · cited 7×
“Delivery format is also relevant as consumption of free-form amino acids (i.e., free-form EAAs) leads to an early appearance and higher concentration of plasma amino acids compared with intact protein sources (69) and can elicit a greater anabolic stimulus compared with mixed meals requiring digestion (70).”
BackgroundInfluentialMyostatin, a Negative Regulator of Muscle Growth, Functions by Inhibiting Myoblast Proliferation
Thomas, M., Langley, B., Berry, C., Sharma, M., Kirk, S., Bass, J., & Kambadur, R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m004356200 · Cited by 1,014
Tang, J. E., Moore, D. R., Kujbida, G. W., Tarnopolsky, M. A., & Phillips, S. M. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00076.2009 · Cited by 998
Volpi, E., Kobayashi, H., Sheffield-Moore, M., Mittendorfer, B., & Wolfe, R. R. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.2.250 · Cited by 846
IL-6-induced skeletal muscle atrophy
Haddad, F., Zaldivar, F., Cooper, D. M., & Adams, G. R. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01026.2004 · Cited by 612
Effect of physiologic hyperinsulinemia on skeletal muscle protein synthesis and breakdown in man.
Gelfand, R. A., & Barrett, E. J. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113033 · Cited by 522
Research works printed but not linked: 71
- Suetta, 2009, Effects of aging on human skeletal muscle after immobilization and retraining, J Appl Physiol (1985), 107, 1172, 10.1152/japplphysiol.00290.2009 · DOI · Paper page
- Thomas, 2016, Muscle atrophy contributes to quadriceps weakness after anterior cruciate ligament reconstruction, J Sci Med Sport, 19, 7, 10.1016/j.jsams.2014.12.009 · DOI
- Agten, 2017, MR imaging of soft tissue alterations after total hip arthroplasty: comparison of classic surgical approaches, Eur Radiol, 27, 1312, 10.1007/s00330-016-4455-7 · DOI
- Muyskens, 2016, Transcriptional profiling and muscle cross-section analysis reveal signs of ischemia reperfusion injury following total knee arthroplasty with tourniquet, Physiol Rep, 4, e12671, 10.14814/phy2.12671 · DOI
- Kobayashi, 2004, Muscle performance after anterior cruciate ligament reconstruction, Int Orthop, 28, 48, 10.1007/s00264-003-0502-5 · DOI
- Rice, 2010, Quadriceps arthrogenic muscle inhibition: neural mechanisms and treatment perspectives, Semin Arthritis Rheum, 40, 250, 10.1016/j.semarthrit.2009.10.001 · DOI · Paper page
- Spencer, 1984, Knee joint effusion and quadriceps reflex inhibition in man, Arch Phys Med Rehabil, 65, 171
- Wall, 2014, Substantial skeletal muscle loss occurs during only 5 days of disuse, Acta Physiol (Oxf), 210, 600, 10.1111/apha.12190 · DOI · Paper page
- Campbell, 2013, Skeletal muscle adaptations to physical inactivity and subsequent retraining in young men, Biogerontology, 14, 247, 10.1007/s10522-013-9427-6 · DOI
- Hvid, 2013, Four days of muscle disuse impairs single fiber contractile function in young and old healthy men, Exp Gerontol, 48, 154, 10.1016/j.exger.2012.11.005 · DOI
- Hvid, 2011, Effects of ageing on single muscle fibre contractile function following short-term immobilisation, J Physiol, 589, 4745, 10.1113/jphysiol.2011.215434 · DOI · Paper page
- Brocca, 2015, Human skeletal muscle fibre contractile properties and proteomic profile: adaptations to 3 weeks of unilateral lower limb suspension and active recovery, J Physiol, 593, 5361, 10.1113/JP271188 · DOI
- Greenhaff, 2008, Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle, Am J Physiol Endocrinol Metab, 295, E595, 10.1152/ajpendo.90411.2008 · DOI · Paper page
- Biolo, 1997, An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein, Am J Physiol, 273, E122
- Gibson, 1987, Decrease in human quadriceps muscle protein turnover consequent upon leg immobilization, Clin Sci (Lond), 72, 503, 10.1042/cs0720503 · DOI
- Wall, 2016, Short-term muscle disuse lowers myofibrillar protein synthesis rates and induces anabolic resistance to protein ingestion, Am J Physiol Endocrinol Metab, 310, E137, 10.1152/ajpendo.00227.2015 · DOI · Paper page
- Ferrando, 1996, Prolonged bed rest decreases skeletal muscle and whole body protein synthesis, Am J Physiol, 270, E627
- Glover, 2008, Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion, J Physiol, 586, 6049, 10.1113/jphysiol.2008.160333 · DOI · Paper page
- Tanner, 2015, Age-related differences in lean mass, protein synthesis and skeletal muscle markers of proteolysis after bed rest and exercise rehabilitation, J Physiol, 593, 4259, 10.1113/JP270699 · DOI · Paper page
- Biolo, 2017, Anabolic resistance assessed by oral stable isotope ingestion following bed rest in young and older adult volunteers: relationships with changes in muscle mass, Clin Nutr, 36, 1420, 10.1016/j.clnu.2016.09.019 · DOI
- Dirks, 2018, Interventional strategies to combat muscle disuse atrophy in humans: focus on neuromuscular electrical stimulation and dietary protein, J Appl Physiol (1985), 125, 850, 10.1152/japplphysiol.00985.2016 · DOI
- Bienso, 2012, GLUT4 and glycogen synthase are key players in bed rest-induced insulin resistance, Diabetes, 61, 1090, 10.2337/db11-0884 · DOI
- Richter, 1989, Insulin action in human thighs after one-legged immobilization, J Appl Physiol (1985), 67, 19, 10.1152/jappl.1989.67.1.19 · DOI
- Drummond, 2012, Bed rest impairs skeletal muscle amino acid transporter expression, mTORC1 signaling, and protein synthesis in response to essential amino acids in older adults, Am J Physiol Endocrinol Metab, 302, E1113, 10.1152/ajpendo.00603.2011 · DOI · Paper page
- Wall, 2013, Disuse impairs the muscle protein synthetic response to protein ingestion in healthy men, J Clin Endocrinol Metab, 98, 4872, 10.1210/jc.2013-2098 · DOI
- de Boer, 2007, The temporal responses of protein synthesis, gene expression and cell signalling in human quadriceps muscle and patellar tendon to disuse, J Physiol, 585, 241, 10.1113/jphysiol.2007.142828 · DOI · Paper page
- Urso, 2006, Analysis of human skeletal muscle after 48 h immobilization reveals alterations in mRNA and protein for extracellular matrix components, J Appl Physiol (1985), 101, 1136, 10.1152/japplphysiol.00180.2006 · DOI
- Guan, 1997, Role of focal adhesion kinase in integrin signaling, Int J Biochem Cell Biol, 29, 1085, 10.1016/S1357-2725(97)00051-4 · DOI · Paper page
- Wilkinson, 2008, Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle, J Physiol, 586, 3701, 10.1113/jphysiol.2008.153916 · DOI · Paper page
- Fluck, 2014, Early changes in costameric and mitochondrial protein expression with unloading are muscle specific, Biomed Res Int, 2014, 519310, 10.1155/2014/519310 · DOI
- Crossland, 2013, Focal adhesion kinase is required for IGF-I-mediated growth of skeletal muscle cells via a TSC2/mTOR/S6K1-associated pathway, Am J Physiol Endocrinol Metab, 305, E183, 10.1152/ajpendo.00541.2012 · DOI
- Gan, 2006, Association of focal adhesion kinase with tuberous sclerosis complex 2 in the regulation of s6 kinase activation and cell growth, J Biol Chem, 281, 37321, 10.1074/jbc.M605241200 · DOI
- Kelleher, 2015, REDD2 expression in rat skeletal muscle correlates with nutrient-induced activation of mTORC1: responses to aging, immobilization, and remobilization, Am J Physiol Endocrinol Metab, 308, E122, 10.1152/ajpendo.00341.2014 · DOI
- Symons, 2009, Artificial gravity maintains skeletal muscle protein synthesis during 21 days of simulated microgravity, J Appl Physiol (1985), 107, 34, 10.1152/japplphysiol.91137.2008 · DOI · Paper page
- Dirks, 2014, Neuromuscular electrical stimulation prevents muscle disuse atrophy during leg immobilization in humans, Acta Physiol (Oxf), 210, 628, 10.1111/apha.12200 · DOI
- Jones, 2004, Disuse atrophy and exercise rehabilitation in humans profoundly affects the expression of genes associated with the regulation of skeletal muscle mass, FASEB J, 18, 1025, 10.1096/fj.03-1228fje · DOI · Paper page
- Suetta, 2012, Aging affects the transcriptional regulation of human skeletal muscle disuse atrophy, PLoS One, 7, e51238, 10.1371/journal.pone.0051238 · DOI
- Moller, 2019, Immobilization decreases FOXO3a phosphorylation and increases autophagy-related gene and protein expression in human skeletal muscle, Front Physiol, 10, 736, 10.3389/fphys.2019.00736 · DOI
- Mortensen, 2014, Physical inactivity affects skeletal muscle insulin signaling in a birth weight-dependent manner, J Diabetes Complications, 28, 71, 10.1016/j.jdiacomp.2013.09.002 · DOI
- Atherton, 2016, Control of skeletal muscle atrophy in response to disuse: clinical/preclinical contentions and fallacies of evidence, Am J Physiol Endocrinol Metab, 311, E594, 10.1152/ajpendo.00257.2016 · DOI
- Holloway, 2019, A novel amino acid composition ameliorates short-term muscle disuse atrophy in healthy young men, Front Nutr, 6, 105, 10.3389/fnut.2019.00105 · DOI
- Arentson-Lantz, 2019, Improving dietary protein quality reduces the negative effects of physical inactivity on body composition and muscle function, J Gerontol A Biol Sci Med Sci, 74, 1605, 10.1093/gerona/glz003 · DOI
- Mitchell, 2018, Impact of dairy protein during limb immobilization and recovery on muscle size and protein synthesis; a randomized controlled trial, J Appl Physiol (1985), 124, 717, 10.1152/japplphysiol.00803.2017 · DOI
- Dirks, 2014, Skeletal muscle disuse atrophy is not attenuated by dietary protein supplementation in healthy older men, J Nutr, 144, 1196, 10.3945/jn.114.194217 · DOI · Paper page
- Backx, 2018, Leucine supplementation does not attenuate skeletal muscle loss during leg immobilization in healthy, young men, Nutrients, 10, E635, 10.3390/nu10050635 · DOI
- Trappe, 2007, Influence of concurrent exercise or nutrition countermeasures on thigh and calf muscle size and function during 60 days of bed rest in women, Acta Physiol (Oxf), 191, 147, 10.1111/j.1748-1716.2007.01728.x · DOI · Paper page
- Borsheim, 2002, Essential amino acids and muscle protein recovery from resistance exercise, Am J Physiol Endocrinol Metab, 283, E648, 10.1152/ajpendo.00466.2001 · DOI · Paper page
- Gropper, 1991, Effect of simultaneous ingestion of L-amino acids and whole protein on plasma amino acid and urea nitrogen concentrations in humans, JPEN J Parenter Enteral Nutr, 15, 48, 10.1177/014860719101500148 · DOI
- Stein, 2011, Does protein supplementation prevent muscle disuse atrophy and loss of strength?, Crit Rev Food Sci Nutr, 51, 828, 10.1080/10408398.2010.482679 · DOI
- Burd, 2017, Skeletal muscle remodeling: interconnections between stem cells and protein turnover, Exerc Sport Sci Rev, 45, 187, 10.1249/JES.0000000000000117 · DOI
- Deger, 2017, Systemic inflammation is associated with exaggerated skeletal muscle protein catabolism in maintenance hemodialysis patients, JCI Insight, 2, 95185, 10.1172/jci.insight.95185 · DOI
- Gore, 1993, Acute response of human muscle protein to catabolic hormones, Ann Surg, 218, 679, 10.1097/00000658-199321850-00015 · DOI
- Biolo, 2007, Calorie restriction accelerates the catabolism of lean body mass during 2 wk of bed rest, Am J Clin Nutr, 86, 366, 10.1093/ajcn/86.2.366 · DOI
- Weijzen, 2019, Protein intake falls below 0.6 g·kg-1·d-1 in healthy, older patients admitted for elective hip or knee arthroplasty, J Nutr Health Aging, 23, 299, 10.1007/s12603-019-1157-2 · DOI
- Ferrando, 1997, Resistance exercise maintains skeletal muscle protein synthesis during bed rest, J Appl Physiol (1985), 82, 807, 10.1152/jappl.1997.82.3.807 · DOI · Paper page
- Oates, 2010, Low-volume resistance exercise attenuates the decline in strength and muscle mass associated with immobilization, Muscle Nerve, 42, 539, 10.1002/mus.21721 · DOI
- Arentson-Lantz, 2019, 2,000 Steps/day does not fully protect skeletal muscle health in older adults during bed rest, J Aging Phys Act, 27, 191, 10.1123/japa.2018-0093 · DOI
- Devries, 2015, Low-load resistance training during step-reduction attenuates declines in muscle mass and strength and enhances anabolic sensitivity in older men, Physiol Rep, 3, e12493, 10.14814/phy2.12493 · DOI · Paper page
- Bamman, 1998, Impact of resistance exercise during bed rest on skeletal muscle sarcopenia and myosin isoform distribution, J Appl Physiol (1985), 84, 157, 10.1152/jappl.1998.84.1.157 · DOI · Paper page
- Elliott, 2012, The central role of myostatin in skeletal muscle and whole body homeostasis, Acta Physiol (Oxf), 205, 324, 10.1111/j.1748-1716.2012.02423.x · DOI
- McFarlane, 2006, Myostatin induces cachexia by activating the ubiquitin proteolytic system through an NF-kappaB-independent, FoxO1-dependent mechanism, J Cell Physiol, 209, 501, 10.1002/jcp.20757 · DOI · Paper page
- Wall, 2012, Neuromuscular electrical stimulation increases muscle protein synthesis in elderly type 2 diabetic men, Am J Physiol Endocrinol Metab, 303, E614, 10.1152/ajpendo.00138.2012 · DOI
- Hauger, 2018, Neuromuscular electrical stimulation is effective in strengthening the quadriceps muscle after anterior cruciate ligament surgery, Knee Surg Sports Traumatol Arthrosc, 26, 399, 10.1007/s00167-017-4669-5 · DOI
- Moran, 2019, Functional electrical stimulation following anterior cruciate ligament reconstruction: a randomized controlled pilot study, J Neuroeng Rehabil, 16, 89, 10.1186/s12984-019-0566-0 · DOI
- Holm, 2010, Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle, Am J Physiol Endocrinol Metab, 298, E257, 10.1152/ajpendo.00609.2009 · DOI · Paper page
- Holm, 2008, Changes in muscle size and MHC composition in response to resistance exercise with heavy and light loading intensity, J Appl Physiol (1985), 105, 1454, 10.1152/japplphysiol.90538.2008 · DOI · Paper page
- Bechshoeft, 2013, The anabolic potential of dietary protein intake on skeletal muscle is prolonged by prior light-load exercise, Clin Nutr, 32, 236, 10.1016/j.clnu.2012.06.015 · DOI
- Dreyer, 2013, Essential amino acid supplementation in patients following total knee arthroplasty, J Clin Invest, 123, 4654, 10.1172/JCI70160 · DOI
- Dreyer, 2018, Essential amino acid supplementation mitigates muscle atrophy after total knee arthroplasty: a randomized, double-blind, placebo-controlled trial, JBJS Open Access, 3, e0006, 10.2106/JBJS.OA.18.00006 · DOI
- Ferrando, 2013, Increased nitrogen intake following hip arthroplasty expedites muscle strength recovery, J Aging Res Clin Pract, 2, 369
- Nishizaki, 2015, Effects of supplementation with a combination of beta-hydroxy-beta-methyl butyrate, L-arginine, and L-glutamine on postoperative recovery of quadriceps muscle strength after total knee arthroplasty, Asia Pac J Clin Nutr, 24, 412
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Alcock, R., Hislop, M., Vidgen, H. A., & Desbrow, B. (2024) · Journal of Functional Morphology and Kinesiology · doi:10.3390/jfmk9040221 · Cited by 33
Metadata
- Authors
- Howard, E. E., Pasiakos, S. M., Fussell, M. A., & Rodriguez, N. R.
- ORCID
- Howard, Emily E
- Venue
- Advances in Nutrition
- Publisher
- Elsevier BV
- Published
- 2020-07
- Volume
- 11
- Issue
- 4
- Pages
- 989-1001
- ISSN
- 2161-8313
- Cited by
- 66
- References
- 27
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 575 d)
- Funders
- National Institute of Food and Agriculture (1016873)
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- Howard, E. E., Pasiakos, S. M., Fussell, M. A., & Rodriguez, N. R. (2020). Skeletal Muscle Disuse Atrophy and the Rehabilitative Role of Protein in Recovery from Musculoskeletal Injury. Advances in Nutrition. https://doi.org/10.1093/advances/nmaa015
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