The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study
Open accessAbstract
Background Galactooligosaccharides are selectively fermented by the beneficial member of the colonic microflora contributing to the health of the host.Objective We assessed the prebiotic potential of a novel galactooligosaccharide produced through the action of beta-galactosidases, originating from a probiotic Bifidobacterium bifidum strain, against a galactooligosaccharide produced through the action of an industrial beta-galactosidase and a placebo.Design Fifty-nine healthy human volunteers participated in this study. Initially, the effect of the matrix on the prebiotic properties of a commercially available galactooligosaccharide (7 g/d) was assessed during 7-d treatment periods with a 7-d washout period in between. During the second phase, 30 volunteers were assigned to a sequence of treatments (7 d) differing in the amount of the novel galactooligosaccharide (0, 3.6, or 7 g/d). Stools were recovered before and after each intervention, and bacteria numbers were determined by fluorescent in situ hybridization.Results Addition of the novel galactooligosaccharide mixture significantly increased the bifidobacterial population ratio compared with the placebo (P < 0.05), whereas 7 g/d of the novel galactooligosaccharide significantly increased the bifidobacterial ratio compared with the commercial galactooligosaccharide (P < 0.05). Moreover, a significant relation (P < 0.001) between the bifidobacteria proportion and the novel galactooligosaccharide dose (0, 3.6, and 7 g/d) was observed. This relation was similar to the effect of the novel galactooligosaccharide on the prebiotic index of each dose.Conclusions This study showed that galactooligosaccharide mixtures produced with different beta-galactosidases show different prebiotic properties and that, by using enzymes originating from bifidobacterial species, an increase in the bifidogenic properties of the prebiotic product is achievable.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Although not tested with GLP-1RAs, GOS consistently increases Bifidobacterium and Lactobacillus, while reducing potentially pathogenic bacteria such as Bacteroides spp., Clostridium perfringens, Escherichia coli, Prevotella, and Gardnerella [135-138]”
From Nutritional Approaches to Enhance GLP-1 Analogue Therapy in Obesity: A Narrative Review (2025) · cites this paper as [138]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics
Gibson, G. R., & Roberfroid, M. B. (1995) · Journal of Nutrition · doi:10.1093/jn/125.6.1401 · Cited by 7,659
Self-citationAFRC, R. F. (1989) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1989.tb05105.x · Cited by 4,831
Dietary modulation of the human colonic microbiota: updating the concept of prebiotics
Gibson, G. R., Probert, H. M., Loo, J. V., Rastall, R. A., & Roberfroid, M. B. (2004) · Nutrition Research Reviews · doi:10.1079/nrr200479 · Cited by 2,539
Self-citationPrebiotics: The Concept Revisited1,
Roberfroid, M. (2007) · Journal of Nutrition · doi:10.1093/jn/137.3.830s · Cited by 1,502
Manz, W., Amann, R., Ludwig, W., Vancanneyt, M., & SCHLEIFER, K. H. (1996) · Microbiology · doi:10.1099/13500872-142-5-1097 · Cited by 1,176
Franks, A. H., Harmsen, H. J. M., Raangs, G. C., Jansen, G. J., Schut, F., & Welling, G. W. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.9.3336-3345.1998 · Cited by 1,056
Langendijk-Genevaux, P. S., Schut, F., Jansen, G. J., Raangs, G. C., Kamphuis, G. R., Wilkinson, M. H. F., & Welling, G. W. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.8.3069-3075.1995 · Cited by 974
A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides
Rycroft, C. E., Jones, M. R., Gibson, G. R., & Rastall, R. A. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01446.x · Cited by 653
Self-citationSatokari, R. M., Vaughan, E. E., Akkermans, A. D. L., Saarela, M., & de Vos, W. M. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.2.504-513.2001 · Cited by 444
Bouhnik, Y., Raskine, L., Simoneau, G., Vicaut, É., Neut, C., Flourié, B., Brouns, F. J. P. H., & Bornet, F. R. J. (2004) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/80.6.1658 · Cited by 412
Flagged first, then by citations
Cited by
Sorted by citations
Items 201–268 of 268
Prebiotics for irritable bowel syndrome
Quigley, E. M. M. (2009) · Expert Review of Gastroenterology & Hepatology · doi:10.1586/egh.09.46 · Cited by 8
Bhushan, B., Singh, B. P., Kumari, M., Mishra, V., Saini, K., & Singh, D. (2019) · Environmental chemistry for a sustainable world · doi:10.1007/978-3-030-04675-0_7 · Cited by 9
Sato, S., Chinda, D., Shimoyama, T., Iino, C., Kudo, S., Sawada, K., Mikami, T., Nakaji, S., Sakuraba, H., & Fukuda, S. (2022) · Microorganisms · doi:10.3390/microorganisms10091813 · Cited by 9
Shaping the human microbiome with prebiotic foods – current perspectives for continued development
Tuohy, K. M., Brown, D. T., Klinder, A., & Costabile, A. (2010) · Food Science & Technology Bulletin Functional Foods · doi:10.1616/1476-2137.15989 · Cited by 8
Overview of Prebiotics: Membership, Physiological Effects and their Health Attributes
Tungland, B. C. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-814649-1.00007-7 · Cited by 10
Patra, R., Mitra, S., Das, N. C., & Mukherjee, S. (2022) · Springer eBooks · doi:10.1007/978-981-16-8990-1_8 · Cited by 7
Kusmiyati, N., Wahyuningsih, T. D., & Widodo, W. (2018) · Pakistan Journal of Nutrition · doi:10.3923/pjn.2018.405.410 · Cited by 7
Tenorio, M. L. O., Orrego, D., Klotz-Ceberio, B., Palanca, C., & Tortajada, M. (2022) · JSFA reports · doi:10.1002/jsf2.92 · Cited by 6
Cuany, D., Andetsion, F., Fontannaz, X., Bénet, T., Spichtig, V., & Austin, S. (2021) · Journal of AOAC International · doi:10.1093/jaoacint/qsab095 · Cited by 6
Kim, T., Kim, S. H., & Sung, S. H. (2012) · Phytochemical Analysis · doi:10.1002/pca.2362 · Cited by 6
EFSA Panel on Dietetic Products, N. A. A. (. (2011) · EFSA Journal · doi:10.2903/j.efsa.2011.2060 · Cited by 6
Wang, X., Zhong, P., Huang, W., Zhang, S., Zhang, J., Wang, X., Wang, Q., Huang, L., Wang, J., Lu, Y., et al. (2022) · Journal of Dairy Science · doi:10.3168/jds.2021-21701 · Cited by 6
Assis, N. M., Ferreira-Leitão, V. S., & da Silva, A. S. (2024) · Process Biochemistry · doi:10.1016/j.procbio.2024.12.026 · Cited by 5
Galacto-Oligosaccharides Exert Bifidogenic Effects at Capsule-Compatible Ultra-Low Doses
Harthoorn, L. F., Heyse, J., Baudot, A., van Hengel, I. A. J., & den Abbeele, P. V. (2025) · Metabolites · doi:10.3390/metabo15080530 · Cited by 6
Sriphannam, W. (2012) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb11.3771 · Cited by 5
EFSA Panel on Dietetic Products, N. A. A. (. (2012) · EFSA Journal · doi:10.2903/j.efsa.2012.2854 · Cited by 5
An improved method for galactosyl oligosaccharide characterization
Patil, S., & Rohrer, J. S. (2021) · Journal of Chromatography B · doi:10.1016/j.jchromb.2021.122967 · Cited by 5
Prebiotics, Probiotics, and Synbiotics: A Bifidobacterial View
Morelli, L., Callegari, M. L., & Patrone, V. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-805060-6.00017-x · Cited by 6
Tzortzis, G. (2010) · Wiley eBooks · doi:10.1002/9780470958186.ch12 · Cited by 5
Self-citationGough, L. A., Weldon, A., Clark, C. C. T., Young, A., Roberts, C. J., Clarke, N. D., Brown, M. A., & Williams, R. (2025) · Nutrients · doi:10.3390/nu17213390 · Cited by 4
Bonnet, N., Capeding, M. R., Siegwald, L., Garcia‐Garcerà, M., Desgeorges, T., Tytgat, H. L. P., Krattinger, L.-F., Lebumfacil, J. D., Phee, L., Moll, J. M., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-66930-2 · Cited by 5
Kumari, N., Jassal, S., Kumar, A., & Kumar, A. R. (2025) · Archives of Microbiology · doi:10.1007/s00203-025-04446-5 · Cited by 4
Biopurification of Oligosaccharides by Immobilized Kluyveromyces Lactis
Yeo, I.-S., Yoon, Y., Seo, N., An, H. J., & Kim, J. (2019) · Applied Sciences · doi:10.3390/app9142845 · Cited by 4
In VivoAssessment of the Bioactivity of Food Oligosaccharides
Clemente, A. (2014) · Wiley eBooks · doi:10.1002/9781118817360.ch14 · Cited by 4
Balliett, M., Rasmussen, O., & Burke, J. R. (2011) · Journal of Chiropractic Medicine · doi:10.1016/j.jcm.2011.06.005 · Cited by 3
Enrichment of foods with prebiotics
Stegmayer, M. Á., Sirini, N., Frizzo, L. S., Fernández‐López, J., Pérez‐Álvarez, J. Á., Rosmini, M. R., & Soto, L. P. (2023) · Elsevier eBooks · doi:10.1016/b978-0-443-15346-4.00007-0 · Cited by 3
EFSA Panel on Dietetic Products, N. A. A. (. (2014) · EFSA Journal · doi:10.2903/j.efsa.2014.3756 · Cited by 3
EFSA Panel on Dietetic Products, N. A. A. (. (2011) · EFSA Journal · doi:10.2903/j.efsa.2011.2472 · Cited by 3
Bhattacharjee, C., & Sen, D. (2015) · Springer eBooks · doi:10.1007/978-3-642-40872-4_1854-1 · Cited by 3
Probable Interaction Between Warfarin and Banana Flakes Supplement
King, A. E., & Strnad, K. (2015) · Nutrition in Clinical Practice · doi:10.1177/0884533615591056 · Cited by 2
Milano, A. M., & Arreola, S. L. (2023) · International Food Research Journal · doi:10.47836/ifrj.30.6.08 · Cited by 2
Michalak, M. (2014) · Cited by 2
Prebiotic Profiling of Indigenous Selected Dioscorea Spp. Using In-vitro Techniques
Aswani, M. A., Kathade, S. A., Nisar, A., Anand, P. K., Kunchiraman, B. N., & Jagtap, S. (2022) · Biosciences Biotechnology Research Asia · doi:10.13005/bbra/2993 · Cited by 2
Prebiotic Ingredients in Probiotic Dairy Products
Wang, X., & Rastall, R. A. (2017) · Wiley eBooks · doi:10.1002/9781119214137.ch7 · Cited by 2
“Gut” to grips with the science of the microbiome – a symposium report
Finnegan, Y. E., Neill, H. R., Prpa, E. J., & Pot, B. (2024) · Gut Microbiome · doi:10.1017/gmb.2024.8 · Cited by 2
Parker, C. J., Abbott, S., Butterfield, L., Hunter, K. A., Johnson, M. A., Sharpe, G. R., & Williams, N. C. (2026) · Experimental Physiology · doi:10.1113/ep092682 · Cited by 1
Post-Genomics Approaches towards Monitoring Changes within the Microbial Ecology of the Gut
Tuohy, K. M., Abecia, L., Deaville, E. R., Fava, F., Klinder, A., & Shen, Q. (2009) · Springer eBooks · doi:10.1007/978-0-387-79058-9_3 · Cited by 1
(EFSA), E. F. S. A. (2009) · EFSA Journal · doi:10.2903/j.efsa.2009.1107 · Cited by 1
Tuohy, K. M., Brown, D. T., Klinder, A., Costabile, A., & Fava, F. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-407825-3.00005-8 · Cited by 2
EFSA Panel on Dietetic Products, N. A. A. (. (2013) · EFSA Journal · doi:10.2903/j.efsa.2013.3259 · Cited by 1
Role of the Gut Microbiota in Maintaining GI Health: Highlights on Inflammatory Bowel Disease
Gruber, L., & Haller, D. (2014) · Molecular and integrative toxicology · doi:10.1007/978-1-4471-6539-2_13 · Cited by 1
Martínez, Y., Iser, M., Valdivié, M., Galindo, J., & Sánchez-Chiprés, D. (2021) · Revista Mexicana de Ciencias Pecuarias · doi:10.22319/rmcp.v12i3.5892 · Cited by 1
Synbiot encapsulation employing a pea protein-alginate matrix
Klemmer, K. J. (2011) · University Library - University of Saskatchewan (University of Saskatchewan) · Cited by 1
Hernández‐Hernández, O. (2012) · Biblos-e Archivo (Universidad Autónoma de Madrid) · Cited by 1
Walton, G., Lim, Y. N., Ng, Y. K., Hannah, R., & Hunter, K. A. (2010) · Food Science & Technology Bulletin Functional Foods · doi:10.1616/1476-2137.15860 · Cited by 1
The gut in control of health and disease
van der Beek (2017) · Research Publications (Maastricht University) · doi:10.26481/dis.20171004cvdb · Cited by 1
Smith, M. L. (2018) · SUNScholar (Stellenbosch University) · Cited by 1
Bridging the immune gap between human milk and infant formula : non-digestible oligosaccharides
Logtenberg, M. J. (2020) · doi:10.18174/524463 · Cited by 1
Exploitation of Exopolysaccharides from Lactic Acid Bacteria
TSUDA, H. (2020) · Wiley eBooks · doi:10.1002/9783527818310.ch4 · Cited by 1
Röytiö, H., Tiihonen, K., & Ouwehand, A. C. (2012) · Wiley eBooks · doi:10.1002/9781118373941.ch3 · Cited by 1
Role of Fermented Dairy Products in Enhancing the Immunity
Dasriya, V. L., Ranveer, S. A., Bajaj, R., Sharma, A., Dasriya, Y., & Dhillon, H. S. (2023) · doi:10.1002/9781394229116.ch6 · Cited by 1
Toward Second‐Generation Carbohydrate Functional Food Ingredients
Rastall, R. A. (2010) · doi:10.1002/9780470958186.ch9
Macronutrients and type 1 diabetes (revision number 6)
Garden, L., & Paterson, K. (2014) · Diapedia.org eBooks · doi:10.14496/dia.8105345813.6
Villa, C. (2012) · TSpace (University of Toronto)
THE CARBOHYDRATE PREBIOTICS AND PROSPECTS OF THEIR USE
Poltavs'ka, O. A., & Коваленко, Н. К. (2013) · Microbiology&Biotechnology · doi:10.18524/2307-4663.2013.4(24).48949
Wen, B. (2015) · TSpace (University of Toronto)
de Boer, R. (2014) · doi:10.1002/9781118598634.ch7
Prebiotics Ingestion in Relevance to Lactose Intolerance AlleviationPreprint
Abd-ElSalam, R., & Kielė, V. (2024) · Research Square · doi:10.21203/rs.3.rs-4722898/v1
Bonnet, N., Capeding, M. R., Siegwald, L., Garcia‐Garcerà, M., Desgeorges, T., Tytgat, H. L. P., Krattinger, L.-F., Lebumfacil, J. D., Phee, L., Moll, J. M., et al. (2024) · medRxiv · doi:10.1101/2024.12.11.24318836
Macůrková, A., Bleha, R., Synytsya, A., Krausová, G., Štětina, J., Němečková, I., & Čurda, L. (2026) · SSRN Electronic Journal · doi:10.2139/ssrn.6591045
Personalized medicine and microbiome integration
Acharya, S., Zafar, S. S., Khan, M. K. A., & Aziz, M. A. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-40551-8.00018-4
Macůrková, A., Bleha, R., Synytsya, A., Krausová, G., Štětina, J., Němečková, I., & Čurda, L. (2026) · Carbohydrate Research · doi:10.1016/j.carres.2026.110023
Bhattacharjee, C., & Sen, D. (2016) · Springer eBooks · doi:10.1007/978-3-662-44324-8_1854
Sage, S. A., Abbott, S. J., Korczak, R., & Williams, N. C. (2026) · Journal of the International Society of Sports Nutrition · doi:10.1080/15502783.2026.2748763
Bajwa, A. A., Iqbal, S., Nasir, M., & Anjum, A. A. (2026) · AIMS Medical Science · doi:10.3934/medsci.2026016
Gorini, F., Tonacci, A., Gabriele, M., & Borghini, A. (2026) · Nutrients · doi:10.3390/nu18213535
Metadata
- Authors
- Depeint, F., Tzortzis, G., Vulevic, J., I'Anson, K., & Gibson, G. R.
- Affiliations
- Quadram Institute (GB)
- Corresponding author
- Tzortzis, George
- Venue
- The American Journal of Clinical Nutrition
- Publisher
- Elsevier BV
- Published
- 2008-03
- Volume
- 87
- Issue
- 3
- Pages
- 785-791
- ISSN
- 0002-9165 (ISSN-L)
- OpenAlex
- W2153503726
- Cited by
- 268
- References
- 25
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 10 · 2023: 11 · 2022: 15 · 2021: 15 · 2020: 20 · 2019: 11 · 2018: 22 · 2017: 19 · 2016: 18 · 2015: 15 · 2014: 13 · 2013: 11 · 2012: 14
- FWCI
- 17.81
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- American Journal of Clinical Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- galactooligosaccharides, prebiotics, Bifidobacterium bifidum, beta-galactosidase, bifidogenic effect, Bifidobacterium, colonic microbiota, prebiotic index, dose-response relationship
- MeSH terms
- Adult, beta-Galactosidase, Bifidobacterium, Colon, Dose-Response Relationship, Drug, Double-Blind Method, Feces, Female, Fermentation, Galactose, Humans, Male, Oligosaccharides, In Situ Hybridization, Fluorescence, Cross-Over Studies, Probiotics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
