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Gene regulation inference from single-cell RNA-seq data with linear differential equations and velocity inference
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Abstract
Motivation: Single-cell RNA sequencing (scRNA-seq) offers new possibilities to infer gene regulatory network (GRNs) for biological processes involving a notion of time, such as cell differentiation or cell cycles. It also raises many challenges due to the destructive measurements inherent to the technology. Results: In this work, we propose a new method named GRISLI for de novo GRN inference from scRNA-seq data. GRISLI infers a velocity vector field in the space of scRNA-seq data from profiles of individual cells, and models the dynamics of cell trajectories with a linear ordinary differential equation to reconstruct the underlying GRN with a sparse regression procedure. We show on real data that GRISLI outperforms a recently proposed state-of-the-art method for GRN reconstruction from scRNA-seq data. Availability and implementation: The MATLAB code of GRISLI is available at: https://github.com/PCAubin/GRISLI. Supplementary information: Supplementary data are available at Bioinformatics online.
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“Single-cell RNA sequencing (scRNA-seq) data enables cell type-specific trans-regulation inference through co-expression analysis such as PIDC and SCENIC21–30.”
From Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data (2025) · cites this paper as [27]
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Cites
Scenic: single-cell regulatory network inference and clustering.
Aibar, S., González-Blas, C. B., Moerman, T., Huynh-Thu, V. A., Imrichova, H., Hulselmans, G., Rambow, F., Marine, J.-C., Geurts, P., Aerts, J., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4463 · Cited by 6,520
Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma
Patel, A. P., Tirosh, I., Trombetta, J. J., Shalek, A. K., Gillespie, S. M., Wakimoto, H., Cahill, D. P., Nahed, B. V., Curry, W. T., Martuza, R. L., et al. (2014) · Science · doi:10.1126/science.1254257 · Cited by 5,063
La Manno, G., Soldatov, R., Zeisel, A., Braun, E., Hochgerner, H., Petukhov, V., Lidschreiber, K., Kastriti, M. E., Lönnerberg, P., Furlan, A., et al. (2018) · Nature · doi:10.1038/s41586-018-0414-6 · Cited by 4,118
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq
Zeisel, A., Muñoz-Manchado, A. B., Codeluppi, S., Lönnerberg, P., La Manno, G., Juréus, A., Marques, S., Munguba, H., He, L., Betsholtz, C., et al. (2015) · Science · doi:10.1126/science.aaa1934 · Cited by 3,124
Generalizing RNA velocity to transient cell states through dynamical modeling
Bergen, V., Lange, M., Peidli, S., Wolf, F. A., & Theis, F. J. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0591-3 · Cited by 2,951
Meinshausen, N., & Bühlmann, P. (2010) · Journal of the Royal Statistical Society Series B (Statistical Methodology) · doi:10.1111/j.1467-9868.2010.00740.x · Cited by 2,463
Inferring Regulatory Networks from Expression Data Using Tree-Based Methods
Huynh-Thu, V. A., Irrthum, A., Wehenkel, L., & Geurts, P. (2010) · PLoS ONE · doi:10.1371/journal.pone.0012776 · Cited by 2,131
Adult mouse cortical cell taxonomy revealed by single cell transcriptomics
Tasic, B., Menon, V., Nguyen, T. N., Kim, T. K., Jarsky, T., Yao, Z., Levi, B., Gray, L. T., Sorensen, S. A., Dolbeare, T., et al. (2016) · Nature Neuroscience · doi:10.1038/nn.4216 · Cited by 1,714
Wisdom of crowds for robust gene network inference
The DREAM5 Consortium, Marbach, D., Costello, J. C., Küffner, R., Vega, N. M., Prill, R. J., Camacho, D. M., Allison, K. R., Kellis, M., Collins, J. J., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2016 · Cited by 1,667
The Technology and Biology of Single-Cell RNA Sequencing
Kolodziejczyk, A. A., Kim, J. K., Svensson, V., Marioni, J. C., & Teichmann, S. A. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.04.005 · Cited by 1,487
Bayesian approach to single-cell differential expression analysis
Kharchenko, P. V., Silberstein, L., & Scadden, D. T. (2014) · Nature Methods · doi:10.1038/nmeth.2967 · Cited by 1,348
Single-Cell RNA-Seq Reveals Dynamic, Random Monoallelic Gene Expression in Mammalian Cells
Deng, Q., Ramsköld, D., Reinius, B., & Sandberg, R. (2014) · Science · doi:10.1126/science.1245316 · Cited by 1,345
Computational and analytical challenges in single-cell transcriptomics
Stegle, O., Teichmann, S. A., & Marioni, J. C. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3833 · Cited by 1,170
Transcriptional Heterogeneity and Lineage Commitment in Myeloid Progenitors
Paul, F., Arkin, Y., Giladi, A., Jaitin, D. A., Kenigsberg, E., Keren-Shaul, H., Winter, D., Lara-Astiaso, D., Gury, M., Weiner, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.11.013 · Cited by 1,082
Hu, H., Miao, Y., Jia, L.-H., Yu, Q.-Y., Zhang, Q., & Guo, A. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky822 · Cited by 816
Defining cell types and states with single-cell genomics
Trapnell, C. (2015) · Genome Research · doi:10.1101/gr.190595.115 · Cited by 813
A general and flexible method for signal extraction from single-cell RNA-seq data
Risso, D., Perraudeau, F., Gribkova, S., Dudoit, S., & Vert, J.-P. (2018) · Nature Communications · doi:10.1038/s41467-017-02554-5 · Cited by 718
Self-citationGene Regulatory Network Inference from Single-Cell Data Using Multivariate Information Measures
Chan, T. E., Stumpf, M. P. H., & Babtie, A. C. (2017) · Cell Systems · doi:10.1016/j.cels.2017.08.014 · Cited by 578
Chu, L.-F., Leng, N., Zhang, J., Hou, Z., Mamott, D., Vereide, D. T., Choi, J., Kendziorski, C., Stewart, R., & Thomson, J. A. (2016) · Genome Biology · doi:10.1186/s13059-016-1033-x · Cited by 477
Circuitry and Dynamics of Human Transcription Factor Regulatory Networks
Neph, S., Stergachis, A. B., Reynolds, A., Sandstrom, R., Borenstein, E., & Stamatoyannopoulos, J. A. (2012) · Cell · doi:10.1016/j.cell.2012.04.040 · Cited by 467
Design and computational analysis of single-cell RNA-sequencing experiments
Bacher, R. L., & Kendziorski, C. M. (2016) · Genome biology · doi:10.1186/s13059-016-0927-y · Cited by 460
Dissecting direct reprogramming from fibroblast to neuron using single-cell RNA-seq
Treutlein, B., Lee, Q. Y., Camp, J. G., Mall, M., Koh, W. L. C., Shariati, S. A., Sim, S., Neff, N. F., Skotheim, J. M., Wernig, M., et al. (2016) · Nature · doi:10.1038/nature18323 · Cited by 442
Matsumoto, H., Kiryu, H., Furusawa, C., Ko, M. S. H., Ko, S. B. H., Gouda, N., Hayashi, T., & Nikaido, I. (2017) · Bioinformatics · doi:10.1093/bioinformatics/btx194 · Cited by 439
TIGRESS: Trustful Inference of Gene REgulation using Stability Selection
Haury, A.-C., Mordelet, F., Vera‐Licona, P., & Vert, J. (2012) · BMC Systems Biology · doi:10.1186/1752-0509-6-145 · Cited by 414
Self-citationMoignard, V., Woodhouse, S., Haghverdi, L., Lilly, A. J., Tanaka, Y., Wilkinson, A. C., Buettner, F., Macaulay, I. C., Jawaid, W., Diamanti, E., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3154 · Cited by 380
Structured Sparsity through Convex Optimization
Bach, F., Jenatton, R., Mairal, J., & Obozinski, G. (2012) · Statistical Science · doi:10.1214/12-sts394 · Cited by 335
Moignard, V., Macaulay, I. C., Swiers, G., Buettner, F., Schütte, J., Calero‐Nieto, F. J., Kinston, S., Joshi, A., Hannah, R., Theis, F. J., et al. (2013) · Nature Cell Biology · doi:10.1038/ncb2709 · Cited by 275
Lönnberg, T., Svensson, V., James, K. R., Fernandez‐Ruiz, D., Sebina, I., Montandon, R., Soon, M. S. F., Fogg, L. G., Nair, A. S., Liligeto, U. N., et al. (2017) · Science Immunology · doi:10.1126/sciimmunol.aal2192 · Cited by 260
Comprehensive single cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis
Bastidas-Ponce, A., Tritschler, S., Dony, L., Scheibner, K., Tarquis-Medina, M., Salinno, C., Schirge, S., Burtscher, I., Böttcher, A., Theis, F. J., et al. (2019) · Development · doi:10.1242/dev.173849 · Cited by 250
Bessonnard, S., Mot, L. D., Gonze, D., Barriol, M., Dennis, C., Goldbeter, A., Dupont, G., & Chazaud, C. (2014) · Development · doi:10.1242/dev.109678 · Cited by 221
Predicting causal effects in large-scale systems from observational data
Maathuis, M. H., Colombo, D., Kalisch, M., & Bühlmann, P. (2010) · Nature Methods · doi:10.1038/nmeth0410-247 · Cited by 217
Conservation of trans-acting circuitry during mammalian regulatory evolution
Stergachis, A. B., Neph, S., Sandstrom, R., Haugen, E., Reynolds, A. P., Zhang, M., Byron, R., Canfield, T., Stelhing-Sun, S., Lee, K., et al. (2014) · Nature · doi:10.1038/nature13972 · Cited by 212
Computational methods for trajectory inference from single‐cell transcriptomics
Cannoodt, R., Saelens, W., & Saeys, Y. (2016) · European Journal of Immunology · doi:10.1002/eji.201646347 · Cited by 210
Reconstructing gene regulatory dynamics from high-dimensional single-cell snapshot data
Ocone, A., Haghverdi, L., Mueller, N. S., & Theis, F. J. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv257 · Cited by 155
A genome-wide and nonredundant mouse transcription factor database
Kanamori, M., Konno, H., Osato, N., Kawai, J., Hayashizaki, Y., & Suzuki, H. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.07.179 · Cited by 122
Inferring gene regulatory networks from single-cell data: a mechanistic approach
Herbach, U., Bonnaffoux, A., Espinasse, T., & Gandrillon, O. (2017) · BMC Systems Biology · doi:10.1186/s12918-017-0487-0 · Cited by 83
A Bayesian Nonparametric Approach to Testing for Dependence Between Random Variables
Filippi, S., & Holmes, C. C. (2016) · Bayesian Analysis · doi:10.1214/16-ba1027 · Cited by 33
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Benchmarking algorithms for gene regulatory network inference from single-cell transcriptomic data
Pratapa, A., Jalihal, A. P., Law, J. N., Bharadwaj, A., & Murali, T. M. (2020) · Nature Methods · doi:10.1038/s41592-019-0690-6 · Cited by 792
Applications of single-cell and bulk RNA sequencing in onco-immunology
Kuksin, M., Morel, D., Aglave, M., Danlos, F., Marabelle, A., Zinovyev, A. Y., Gautheret, D., & Verlingue, L. (2021) · European Journal of Cancer · doi:10.1016/j.ejca.2021.03.005 · Cited by 172
Inferring gene regulatory network from single-cell transcriptomes with graph autoencoder model
Wang, J., Chen, Y., & Zou, Q. (2023) · PLoS Genetics · doi:10.1371/journal.pgen.1010942 · Cited by 154
Network inference with Granger causality ensembles on single-cell transcriptomics
Deshpande, A., Chu, L.-F., Stewart, R., & Gitter, A. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.110333 · Cited by 130
Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data
Yuan, Q., & Duren, Z. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02182-7 · Cited by 118
Metadata
- Authors
- Aubin-Frankowski, P.-C., & Vert, J.-P.
- Affiliations
- Université Paris Sciences et Lettres (FR)
- Google (United States) (US)
- Corresponding author
- Aubin-Frankowski, Pierre-Cyril
- Vert, Jean-Philippe
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-06-17
- Volume
- 36
- Issue
- 18
- Pages
- 4774-4780
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- OpenAlex
- W3036077871
- Cited by
- 116
- References
- 38
- Citations by year
- 2026: 9 · 2025: 18 · 2024: 20 · 2023: 23 · 2022: 23 · 2021: 17 · 2020: 4
- FWCI
- 5.44
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Bioinformatics (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- gene regulatory network inference, single-cell RNA sequencing, linear differential equations, sparse regression, cell differentiation, cell cycle
- MeSH terms
- RNA-Seq, Sequence Analysis, RNA, Gene Expression Profiling, Gene Regulatory Networks, Single-Cell Analysis
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