Differential coexpression analysis using microarray data and its application to human cancer
Open accessAbstract
Motivation: Microarrays have been used to identify differential expression of individual genes or cluster genes that are coexpressed over various conditions. However, alteration in coexpression relationships has not been studied. Here we introduce a model for finding differential coexpression from microarrays and test its biological validity with respect to cancer. Results: We collected 10 published gene expression datasets from cancers of 13 different tissues and constructed 2 distinct coexpression networks: a tumor network and normal network. Comparison of the two networks showed that cancer affected many coexpression relationships. Functional changes such as alteration in energy metabolism, promotion of cell growth and enhanced immune activity were accompanied with coexpression changes. Coregulation of collagen genes that may control invasion and metastatic spread of tumor cells was also found. Cluster analysis in the tumor network identified groups of highly interconnected genes related to ribosomal protein synthesis, the cell cycle and antigen presentation. Metallothionein expression was also found to be clustered, which may play a role in apoptosis control in tumor cells. Our results show that this model would serve as a novel method for analyzing microarrays beyond the specific implications for cancer. Supplementary information: Supplementary data are available at Bioinformatics online. Contact: sskimb@ssu.ac.kr
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Differential co-expression or connectivity methods are additional means for determining gene connectivity changes between conditions and can identify disruption or gain of function in pathways.
“Differential co-expression or connectivity methods36,37 are additional means for determining gene connectivity changes between conditions and can identify disruption or gain of function in pathways.”
From Systems biology and gene networks in neurodevelopmental and neurodegenerative disorders (2015) · cites this paper as [37]
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- Authors
- Choi, J. K., Yu, U., Yoo, O. J., & Kim, S.
- Affiliations
- Korea Research Institute of Bioscience and Biotechnology (KR)
- National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology 1 1 52 Ueun-dong, Yuseong-gu, Daejeon, Korea
- Korea Advanced Institute of Science and Technology (KR)
- Soongsil University (KR)
- Corresponding author
- Kim, Sangsoo
- Venue
- Bioinformatics
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-10-18
- Volume
- 21
- Issue
- 24
- Pages
- 4348-4355
- ISSN
- 1367-4803 (ISSN-L)
- 1367-4811
- OpenAlex
- W2139807849
- Cited by
- 265
- References
- 42
- Citations by year
- 2025: 1 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 8 · 2020: 10 · 2019: 9 · 2018: 9 · 2017: 15 · 2016: 13 · 2015: 18 · 2014: 30 · 2013: 20 · 2012: 24
- FWCI
- 4.36
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Bioinformatics (publishedVersion)
- PubMed (publishedVersion)
- KRIBB Repository (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Keywords
- gene co-expression network, cancer, energy metabolism, cell growth, immunological activity, collagen genes, tumor invasion and metastasis, ribosomal protein synthesis, cell cycle, antigen presentation, metallothionein, apoptosis, gene clustering
- MeSH terms
- Algorithms, Data Interpretation, Statistical, Female, Humans, Male, Models, Genetic, Neoplasms, Cluster Analysis, Computational Biology, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Databases, Genetic
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