Process for an efficient lentiviral cell transduction
Open accessAbstract
The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause insufficient representation. Here, we present a protocol that yielded substantial increases in transduction efficiency in various cell lines in comparison to several other procedures.
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Viral methods for introducing genome editing components into cells could traditionally reach efficiencies of 30-50%, but recent modifications have achieved 80-100%.
“Viral methods could traditionally reach efficiencies of 30-50%, but more recent modifications of the transduction method have achieved efficiencies of 80-100% (Pirona et al., 2020; Gu et al., 2022).”
From Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR (2023) · cites this paper as Pirona et al., 2020
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- Authors
- Pirona, A. C., Oktriani, R., Boettcher, M., & Hoheisel, J. D.
- Affiliations
- German Cancer Research Center (DE)
- Universitas Gadjah Mada (ID)
- Martin Luther University Halle-Wittenberg (DE)
- Corresponding author
- Hoheisel, Jörg D
- Venue
- Biology Methods and Protocols
- Publisher
- Oxford University Press (OUP)
- Published
- 2020-01-01
- Volume
- 5
- Issue
- 1
- Pages
- bpaa005-bpaa005
- ISSN
- 2396-8923 (ISSN-L)
- PMC
- PMC7200879
- OpenAlex
- W3009618901
- Cited by
- 31
- References
- 24
- Citations by year
- 2026: 9 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 3 · 2020: 1
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biology Methods and Protocols (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Deutscher Akademischer Austauschdienst (57214224)
- Deutsches Krebsforschungszentrum (Ca173)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- lentiviral vectors, cell transduction, transduction efficiency, CRISPR/Cas9, genome editing, cell lines
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