Advanced transfection with Lipofectamine 2000 reagent: Primary neurons, siRNA, and high-throughput applications
Abstract
Lipofectamine 2000 is a cationic liposome based reagent that provides high transfection efficiency and high levels of transgene expression in a range of mammalian cell types in vitro using a simple protocol. Optimum transfection efficiency and subsequent cell viability depend on a number of experimental variables such as cell density, liposome and DNA concentrations, liposome-DNA complexing time, and the presence or absence of media components such as antibiotics and serum. The importance of these factors in Lipofectamine 2000 mediated transfection will be discussed together with some specific applications: transfection of primary neurons, high throughput transfection, and delivery of small interfering RNAs.
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Lipid-based nanoparticles are composed of a cationic lipid and a helper lipid.
“Lipid-based NPs are composed of a cationic lipid (with a positive charge, a hydrophilic head, and a hydrophobic tail such as DOTAP) and a helper lipid (such as cholesterol) [31].”
From The Ocular Gene Delivery Landscape (2021) · cites this paper as [31]
Cites
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456 · see citing works
Brewer, G. J., Torricelli, J. R., Evege, E. K., & Price, P. J. (1993) · Journal of Neuroscience Research · doi:10.1002/jnr.490350513 · Cited by 2,094 · see citing works
Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
Zabner, J., Fasbender, A., Moninger, T., Poellinger, K. A., & Welsh, M. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.32.18997 · Cited by 1,408 · see citing works
Analysis of gene function in somatic mammalian cells using small interfering RNAs
Elbashir, S. M. (2002) · Methods · doi:10.1016/s1046-2023(02)00023-3 · Cited by 1,094 · see citing works
Short interfering RNA confers intracellular antiviral immunity in human cells
Gitlin, L., Karelsky, S., & Andino, R. (2002) · Nature · doi:10.1038/nature00873 · Cited by 612 · see citing works
Zuhorn, I. S., Kalicharan, R. D., & Hoekstra, D. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111257200 · Cited by 337 · see citing works
Improving the transfection efficiency of post-mitotic neurons
Ohki, E. C., Tilkins, M. L., Ciccarone, V. C., & Price, P. J. (2001) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(01)00441-1 · Cited by 110 · see citing works
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Gold Nanoparticles for Nucleic Acid Delivery
Ding, Y., Jiang, Z., Saha, K., Kim, C. S., Kim, S. T., Landis, R. F., & Rotello, V. M. (2014) · Molecular Therapy · doi:10.1038/mt.2014.30 · Cited by 521 · see citing works
Mechanism of Virus Attenuation by Codon Pair Deoptimization
Groenke, N., Trimpert, J., Merz, S., Conradie, A. M., Wyler, E., Zhang, H., Hazapis, O.-G., Rausch, S., Landthaler, M., Osterrieder, N., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107586 · Cited by 85 · see citing works
Microfluidic platform for hepatitis B viral replication study
Sodunke, T. R., Bouchard, M. J., & Noh, H. (. (2008) · Biomedical Microdevices · doi:10.1007/s10544-007-9148-1 · Cited by 38 · see citing works
Process for an efficient lentiviral cell transduction
Pirona, A. C., Oktriani, R., Boettcher, M., & Hoheisel, J. D. (2020) · Biology Methods and Protocols · doi:10.1093/biomethods/bpaa005 · Cited by 31 · see citing works
The Ocular Gene Delivery Landscape
Sahu, B., Chug, I., & Khanna, H. (2021) · Biomolecules · doi:10.3390/biom11081135 · Cited by 28 · see citing works
Evolution of prime editing systems: move forward to the treatment of hereditary diseases
Volodina, O. V., Fabrichnikova, A. R., Anuchina, A. A., Mishina, O. S., Lavrov, A. V., & Smirnikhina, S. A. (2025) · Current Gene Therapy · doi:10.2174/0115665232295117240405070809 · Cited by 1 · see citing works
- Authors
- Dalby, B.
- Corresponding author
- Dalby, B
- Venue
- Methods
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 33
- Issue
- 2
- Pages
- 95-103
- ISSN
- 1046-2023 (ISSN-L)
- OpenAlex
- W2077112895
- Cited by
- 619
- References
- 7
- Citations by year
- 2026: 12 · 2025: 20 · 2024: 29 · 2023: 25 · 2022: 33 · 2021: 30 · 2020: 40 · 2019: 41 · 2018: 46 · 2017: 23 · 2016: 37 · 2015: 40 · 2014: 29 · 2013: 42 · 2012: 27
- FWCI
- 4.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lipofectamine 2000, cationic liposomes, transfection efficiency, primary neurons, siRNA delivery, cell viability
- MeSH terms
- Animals, beta-Galactosidase, Cell Count, Cells, Cultured, Cerebral Cortex, Culture Media, DNA, Hippocampus, Humans, Lipids, Liposomes, Luciferases, Neurons, Plasmids, Transfection, Genes, Reporter, RNA Interference, RNA, Small Interfering, Lamin Type A, Rats
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