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Cited by 399Skin Protection and AgingTea Polyphenols and EffectsPhytochemicals and Antioxidant Activities

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress

Katiyar, S. K., Afaq, F., Perez, A., Mukhtar, H.·2001·Carcinogenesis
DOI 10.1093/carcin/22.2.287
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Abstract

The use of naturally occurring botanicals with substantial antioxidant activity to afford protection to human skin against UV damage is receiving increasing attention. The green tea constituent (-)-epigallocatechin-3-gallate (EGCG) is a potent antioxidant and has shown remarkable preventive effects against photocarcinogenesis and phototoxicity in mouse models. In this study we have investigated the effects of topical application of EGCG, the major polyphenol present in green tea, to human skin before UV irradiation on UV-induced markers of oxidative stress and antioxidant enzymes. Using immunohistochemistry and analytical enzyme assays, we found that application of EGCG (mg/cm(2) skin) before a single UV exposure of 4x minimal erythema dose (MED) markedly decreases UV-induced production of hydrogen peroxide (68-90%, P < 0.025-0.005) and nitric oxide (30-100%, P < 0.025-0.005) in both epidermis and dermis in a time-dependent manner. EGCG pretreatment also inhibits UV-induced infiltration of inflammatory leukocytes, particularly CD11b(+) cells (a surface marker of monocytes/macrophages and neutrophils), into the skin, which are considered to be the major producers of reactive oxygen species. EGCG treatment was also found to inhibit UV-induced epidermal lipid peroxidation at each time point studied (41-84%, P < 0.05). A single UV exposure of 4x MED to human skin was found to increase catalase activity (109-145%) and decrease glutathione peroxidase (GPx) activity (36-54%) and total glutathione (GSH) level (13-36%) at different time points studied. Pretreatment with EGCG was found to restore the UV-induced decrease in GSH level and afforded protection to the antioxidant enzyme GPx. Further studies are warranted to study the preventive effects of EGCG against multiple exposures to UV light of human skin.

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Crossref · 10.1093/carcin/22.2.287

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Citations

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Show all 53 references

Research works printed but not linked: 10

  1. Young, 1990, Seminars in dermatology, 9, 25
  2. Wang, 1992, Cancer Research, 52, 1162
  3. Wang, 1992, Cancer Research, 52, 6657
  4. Xu, 1992, Cancer Research, 52, 3875
  5. Han, 1990, Biomedical and environmental sciences : BES, 3, 35
  6. Xu, 1990, Biomedical and environmental sciences : BES, 3, 406
  7. Katiyar, 1992, Cancer Research, 52, 6890
  8. Katiyar, 1993, Cancer Research, 53, 5409
  9. Mohandas, 1984, Cancer Research, 44, 5086
  10. Cerutti, 1991, Cancer cells (Cold Spring Harbor, N.Y. : 1989), 3, 1

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Metadata

Authors
Katiyar, S. K., Afaq, F., Perez, A., & Mukhtar, H.
Affiliations
University of Alabama at Birmingham (US)
ORCID
Venue
Carcinogenesis
Publisher
Oxford University Press (OUP)
Published
2001-02-01
Volume
22
Issue
2
Pages
287-294
ISSN
  • 0143-3334 (ISSN-L)
  • 1460-2180
DOI
10.1093/carcin/22.2.287
OpenAlex
W2012908941
Cited by
399
References
53
Citations by year
2026: 2 · 2025: 11 · 2024: 7 · 2023: 7 · 2022: 13 · 2021: 11 · 2020: 15 · 2019: 11 · 2018: 17 · 2017: 12 · 2016: 21 · 2015: 12 · 2014: 17 · 2013: 22 · 2012: 21
FWCI
17.81
Type
journal article
Language
en
Open access
closed
Locations
  • Carcinogenesis (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ohio Cancer Research Associates
  • U.S. Public Health Service
  • National Cancer Institute (R01CA 78809, R01CA078809)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (P30AR039750, P30 AR39750)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Tea Polyphenols and Effects
  • Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
Keywords
epigallocatechin gallate, green tea polyphenols, ultraviolet radiation, skin photoprotection, oxidative stress, hydrogen peroxide, nitric oxide, lipid peroxidation, antioxidant enzymes, catalase, glutathione peroxidase, glutathione, topical application, phototoxicity
MeSH terms
Adult, Catalase, Catechin, Female, Glutathione, Glutathione Peroxidase, Humans, Hydrogen Peroxide, Immunoenzyme Techniques, Leukocytes, Male, Middle Aged, Nitric Oxide, Radiodermatitis, Skin, Tea, Time Factors, Ultraviolet Rays, Lipid Peroxidation, Free Radical Scavengers, Oxidative Stress

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