Horizontal synaptic connections in monkey prefrontal cortex: An in vitro electrophysiological study
Abstract
In monkey dorsolateral prefrontal cortex (PFC), long-distance, horizontally oriented intrinsic axon collaterals interconnect clusters of pyramidal neurons in the supragranular layers. In order to study the electrophysiological responses mediated by these long-distance projections, an in vitro slice preparation of monkey PFC was used to obtain whole-cell patch clamp recordings from layer 3 pyramidal neurons. Using in vivo tracer injections, we found that long-distance projections were well preserved in PFC slices cut in the coronal plane. Postsynaptic currents were evoked by low-intensity electrical extracellular stimulation applied successively to 20-30 discrete sites located up to 2200 micron lateral to the recorded cell. Several criteria were applied to discriminate between mono- and polysynaptic responses. Long-distance monosynaptic connections were mediated by fibers with relatively slow conduction velocity (0.14 m/s). Excitatory postsynaptic currents (EPSCs) evoked by stimulation of short- or long-distance horizontal connections did not differ in kinetic properties. The majority (77%) of the 35 layer 3 PFC neurons studied were monosynaptic targets of long-distance connections. EPSCs mediated by long-distance connections had amplitudes that were similar or even larger than short-distance EPSCs, suggesting that excitatory input provided by the former was relatively robust. For most neurons (87.5%) in which a full complement of monosynaptic EPSCs was evoked by multisite sti…
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Spatial tuning is refined by lateral inhibition from GABAergic interneurons.
“Conversely, she showed evidence that the spatial tuning was refined by lateral inhibition from GABAergic interneurons, whereby pyramidal cells could inhibit their neighbors with dissimilar preferred directions to enhance “signal to noise” (Fig. 2C).”
From Guanfacine’s mechanism of action in treating prefrontal cortical disorders: Successful translation across species (2020) · cites this paper as González-Burgos et al., 2000
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- Authors
- Gonzalez-Burgos, G.
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-01-01
- Volume
- 10
- Issue
- 1
- Pages
- 82-92
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W2160931640
- Cited by
- 169
- References
- 5
- Citations by year
- 2026: 10 · 2025: 3 · 2024: 10 · 2023: 9 · 2022: 6 · 2021: 11 · 2020: 12 · 2019: 7 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 10 · 2013: 3 · 2012: 8
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (MH-00519, MH-51234, MH 45156, R01MH051234, P50MH045156)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- dorsolateral prefrontal cortex, axon collaterals, monosynaptic connections, polysynaptic connections, excitatory postsynaptic currents, conduction velocity, intrinsic functional connectivity
- MeSH terms
- Animals, Electric Stimulation, Electrophysiology, Macaca fascicularis, Male, Neural Pathways, Synapses, Prefrontal Cortex, Pyramidal Cells, Patch-Clamp Techniques, Excitatory Postsynaptic Potentials, In Vitro Techniques
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