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Indirect Assessment of Hydroxymethylglutaryl-CoA Reductase (NADPH) Activity in Liver Tissue
Abstract
We describe an indirect method for assessing variation in 3-hydroxy-3-methylglutaryl-coenzyme A reductase (NADPH) activity in liver tissue. 3-Hydroxy-3-methylglutaryl-CoA and mevalonate concentrations in the tissue homogenate are estimated in terms of absorbances and the ratio between the two is taken as an index of activity of the enzyme, which catalyzes the conversion of 3-hydroxy-3-methylglutaryl-CoA to mevalonate. The ratio increases under conditions in which activity of this enzyme in liver reportedly decreases (e.g., fasting, cholesterol feeding) and decreases under conditions in which the activity of this enzyme reportedly increases (e.g., Triton injection, phenobarbital treatment). We assessed the magnitude of hepatic cholesterogenesis in intact male albino rats by using [1,2-14C] acetate, in fed controls, fasted, cholesterol-fed, Triton-treated, and phenobarbital-treated. The ratio, 3-hydroxy-3-methylglutarylCoA/mevalonate was estimated in liver tissue for all the above groups. The method is simple, reproducible, and does not require any unusual chemicals.
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Cited by
Sharma, S. B., Nasir, A., Prabhu, K. M., Murthy, P. S., & Dev, G. (2003) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(02)00366-5 · Cited by 345
Sandhya, V. G., & Rajamohan, T. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2008.08.030 · Cited by 62
Su, J., Ma, C., Liu, C., Gao, C., Nie, R., & Wang, H. (2016) · Journal of Food Science · doi:10.1111/1750-3841.13252 · Cited by 62
Beneficial effects of coconut water feeding on lipid metabolism in cholesterol-fed rats
Sandhya, V. G., & Rajamohan, T. (2006) · Journal of Medicinal Food · doi:10.1089/jmf.2006.9.400 · Cited by 46
Preetha, P. P., Devi, V. G., & Rajamohan, T. (2014) · Comparative Clinical Pathology · doi:10.1007/s00580-013-1784-7 · Cited by 17
Metadata
- Authors
- Rao, A. V., & Ramakrishnan, S.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 1975-09-01
- Volume
- 21
- Issue
- 10
- Pages
- 1523-1525
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W1898641180
- Cited by
- 279
- References
- 0
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Indirect Assessment of Hydroxymethylglutaryl-CoA Reductase (NADPH) Activity in Liver Tissue. https://app.cochat.ai/papers/10.1093/clinchem/21.10.1523
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- Rao, A. V., & Ramakrishnan, S. (1975). Indirect Assessment of Hydroxymethylglutaryl-CoA Reductase (NADPH) Activity in Liver Tissue. Clinical Chemistry. https://doi.org/10.1093/clinchem/21.10.1523
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