The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Effects of Blood-Processing Protocols on Fetal and Total DNA Quantification in Maternal Plasma
Abstract
Background: Recently, apoptotic cells have been found in plasma obtained by centrifugation of blood from pregnant women, raising the question of what constitutes plasma and whether plasma is truly cell free. We compared the effects of different blood-processing protocols on the quantification, DNA composition, and day-to-day fluctuation of fetal and total DNA in maternal plasma. Methods: Blood samples were collected from healthy pregnant women. The blood sample from each individual was simultaneously processed by different means, including the following: Percoll separation, centrifugation, microcentrifugation, and filtration. The resulting plasma aliquots were subjected to real-time quantitative amplification of the β-globin (for total DNA) and SRY (for fetal DNA) genes. The differences in the β-globin and SRY DNA concentrations and the degree of variation between the various plasma aliquots were assessed statistically. Results: Different protocols of blood processing significantly affected the quantification and the day-to-day fluctuation of total (P <0.001), but not fetal (quantification, P = 0.336; fluctuation, P = 0.206), DNA in maternal plasma. The quantitative difference could be attributed to the fact that efficacies of different protocols for generating cell-free plasma vary. Processing blood samples by centrifugation followed by filtration or microcentrifugation is effective in producing cell-free plasma. Conclusions: Standardization in plasma-processing protocols is needed for maternal plasma DNA analysis, especially for quantification of total DNA in maternal plasma. Such preanalytic factors may also affect other applications of plasma DNA analysis.
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Presence of fetal DNA in maternal plasma and serum
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Self-citationRapid Clearance of Fetal DNA from Maternal Plasma
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Self-citationPrenatal Diagnosis of Fetal RhD Status by Molecular Analysis of Maternal Plasma
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Quantitative Abnormalities of Fetal DNA in Maternal Serum in Preeclampsia
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Fleischhacker, M., & Schmidt, B. (2007) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2006.10.001 · Cited by 851
Detection and Characterization of Placental MicroRNAs in Maternal Plasma
Chim, S. S. C., Shing, T. K. F., Hung, E. C. W., Leung, T.-Y., Lau, T.-K., Chiu, R. W. K., & Dennis Lo, Y. M. (2008) · Clinical Chemistry · doi:10.1373/clinchem.2007.097972 · Cited by 850
Self-citationCirculating cell free DNA: Preanalytical considerations
El Messaoudi, S., Rolet, F., Mouliere, F., & Thierry, A. R. (2013) · Clinica Chimica Acta · doi:10.1016/j.cca.2013.05.022 · Cited by 560
Circulating Methylated SEPT9 DNA in Plasma Is a Biomarker for Colorectal Cancer
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Disorders of sex development: a new definition and classification
Hughes, I. A. (2008) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2007.11.001 · Cited by 438
Metadata
- Authors
- Chiu, R. W. K., Poon, L. L. M., Lau, T. K., Leung, T. N., Wong, E. M. C., & Lo, Y. M. D.
- Venue
- Clinical Chemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-09-01
- Volume
- 47
- Issue
- 9
- Pages
- 1607-1613
- ISSN
- 0009-9147 (ISSN-L)
- 1530-8561
- OpenAlex
- W2108546855
- Cited by
- 351
- References
- 25
- FWCI
- 18.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- Chiu, R. W. K., Poon, L. L. M., Lau, T. K., Leung, T. N., Wong, E. M. C., & Lo, Y. M. D. (2001). Effects of Blood-Processing Protocols on Fetal and Total DNA Quantification in Maternal Plasma. Clinical Chemistry. https://doi.org/10.1093/clinchem/47.9.1607
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