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Cited by 435Calcium signaling and nucleotide metabolismIon Channels and ReceptorsCellular transport and secretion

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

The Golgi apparatus is an inositol 1,4,5‐trisphosphate‐sensitive Ca2+ store, with functional properties distinct from those of the endoplasmic reticulum

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Pinton, Paolo, Pozzan, Tullio, Rizzuto, Rosario·1998·The EMBO Journal
DOI 10.1093/emboj/17.18.5298PMC PMC1170857
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Abstract

In the past few years, intracellular organelles, such as the endoplasmic reticulum, the nucleus and the mitochondria, have emerged as key determinants in the generation and transduction of Ca2+ signals of high spatio-temporal complexity. Little is known about the Golgi apparatus, despite the fact that Ca2+ within its lumen controls essential processes, such as protein processing and sorting. We report the direct monitoring of the [Ca2+] in the Golgi lumen ([Ca2+]Golgi) of living HeLa cells, using a specifically targeted Ca2+-sensitive photoprotein. With this probe, we show that, in resting cells, [Ca2+]Golgi is approximately 0.3 mM and that Ca2+ accumulation by the Golgi has properties distinct from those of the endoplasmic reticulum (as inferred by the sensitivity to specific inhibitors). Upon stimulation with histamine, an agonist coupled to the generation of inositol 1,4,5-trisphosphate (IP3), a large, rapid decrease in [Ca2+]Golgi is observed. The Golgi apparatus can thus be regarded as a bona fide IP3-sensitive intracellular Ca2+ store, a notion with major implications for the control of organelle function, as well as for the generation of local cytosolic Ca2+ signals.

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Metadata

Authors
Pinton, P., Pozzan, T., & Rizzuto, R.
Affiliations
University of Padua (IT)
Corresponding author
Rizzuto, Rosario
ORCID
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
1998-09-15
Volume
17
Issue
18
Pages
5298-5308
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
DOI
10.1093/emboj/17.18.5298
PMC
PMC1170857
OpenAlex
W2111698016
Cited by
435
References
41
Citations by year
2026: 1 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 8 · 2021: 10 · 2020: 11 · 2019: 6 · 2018: 9 · 2017: 11 · 2016: 17 · 2015: 10 · 2014: 18 · 2013: 15 · 2012: 19
FWCI
10.59
Type
journal article
Language
en
Open access
Licence
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
  • Padua Research Archive (University of Padova) (submittedVersion)
Funders
  • Harvard University
  • European Commission
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
Golgi apparatus, inositol 1,4,5-trisphosphate, intracellular calcium stores, endoplasmic reticulum, calcium signaling, histamine stimulation, HeLa cells
MeSH terms
Calcium-Transporting ATPases, Aequorin, Biological Transport, Calcium Chloride, Calcium-Binding Proteins, Endoplasmic Reticulum, Enzyme Inhibitors, Golgi Apparatus, HeLa Cells, Humans, Hydroquinones, Recombinant Fusion Proteins, Ribonucleoproteins, Sialyltransferases, Strontium, Vanadates, Inositol 1,4,5-Trisphosphate, Guanosine 5'-O-(3-Thiotriphosphate), Thapsigargin, Calcium Signaling, Calreticulin

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