Ku protein stimulates DNA end joining by mammalian DNA ligases: a direct role for Ku in repair of DNA double‐strand breaks
Open accessAbstract
Ku protein binds to DNA ends and is a cofactor for the DNA-dependent protein kinase. Both of these components are involved in DNA double-strand break repair, but it has not been clear if they function indirectly, by sensing DNA damage and activating other factors, or if they are more directly involved in the processing and rejoining of DNA breaks. We demonstrate that intermolecular ligation of DNA fragments is highly dependent on Ku under conditions designed to mimic those existing in the cell. This effect of Ku is specific to eukaryotic DNA ligases. Ku protein, therefore, has an activity consistent with a direct role in rejoining DNA breaks and independent of DNA-dependent protein kinase.
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The Ku complex, a Ku70/Ku80 heterodimer, is a major end-binding factor in mammalian cells and has end-bridging activity.
“It has been shown that Ku complex (a heterodimer of Ku70/Ku80 [≈86]) is a major end binding factor in mammalian cells, possess end bridging activity [308, 309].”
From Molecular Mechanisms of Ultraviolet Radiation‐Induced DNA Damage and Repair (2010) · cites this paper as [309]
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- Authors
- Ramsden, D. A., & Gellert, M.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Gellert, Martin
- Venue
- The EMBO Journal
- Published
- 1998-01-15
- Volume
- 17
- Issue
- 2
- Pages
- 609-614
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC1170410
- OpenAlex
- W2011320725
- Cited by
- 296
- References
- 41
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 6 · 2020: 5 · 2019: 7 · 2018: 7 · 2017: 3 · 2016: 5 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 6
- FWCI
- 11.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Cancer Research Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- Ku protein, DNA end joining, DNA double-strand break repair, DNA-dependent protein kinase, DNA end binding, DNA repair
- MeSH terms
- Ku Autoantigen, DNA Ligase ATP, Poly-ADP-Ribose Binding Proteins, DNA, DNA Damage, DNA Repair, DNA Helicases, DNA-Binding Proteins, Humans, Nuclear Proteins, DNA Ligases, Xenopus Proteins, Antigens, Nuclear
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