The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Single-molecule investigation of the interference between kinesin, tau and MAP2c
Open accessAbstract
Motor proteins and microtubule-associated proteins (MAPs) play important roles in cellular transport, regulation of shape and polarity of cells. While motor proteins generate motility, MAPs are thought to stabilize the microtubule tracks. However, the proteins also interfere with each other, such that MAPs are able to inhibit transport of vesicles and organelles in cells. In order to investigate the mechanism of MAP-motor interference in molecular detail, we have studied single kinesin molecules by total internal reflection fluorescence microscopy in the presence of different neuronal MAPs (tau, MAP2c). The parameters observed included run-length (a measure of processivity), velocity and frequency of attachment. The main effect of MAPs was to reduce the attachment frequency of motors. This effect was dependent on the concentration, the affinity to microtubules and the domain composition of MAPs. In contrast, once attached, the motors did not show a change in speed, nor in their run-length. The results suggest that MAPs can regulate motor activity on the level of initial attachment, but not during motion.
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“MAP2 has been shown to block the path of kinesin motors in in vitro assays (46, 47, 48, 49, 50).”
From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [49]
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Cites
The Way Things Move: Looking Under the Hood of Molecular Motor Proteins
Vale, R. D., & Milligan, R. A. (2000) · Science · doi:10.1126/science.288.5463.88 · Cited by 1,503
Bead movement by single kinesin molecules studied with optical tweezers
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Stamer, K., Vogel, R., Thies, E., Mandelkow, E., & Mandelkow, E.-M. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200108057 · Cited by 885
Self-citationEbneth, A., Godemann, R., Stamer, K., Illenberger, S., Trinczek, B., Mandelkow, E.-M., & Mandelkow, E. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.3.777 · Cited by 820
Self-citation[39] Preparation of modified tubulins
Hyman, A. A., Drechsel, D. N., Kellogg, D., Salser, S. J., Sawin, K. E., Steffen, P., Wordeman, L. G., & Mitchison, T. J. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)96041-o · Cited by 728
Direct observation of single kinesin molecules moving along microtubules
Vale, R. D., Funatsu, T., Pierce, D. W., Romberg, L., Harada, Y., & Yanagida, T. (1996) · Nature · doi:10.1038/380451a0 · Cited by 711
Domains of tau Protein and Interactions with Microtubules
Gustke, N., Trinczek, B., Biernat, J., Mandelkow, E.-M., & Mandelkow, E. (1994) · Biochemistry · doi:10.1021/bi00198a017 · Cited by 655
Self-citationProjection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons
Chen, J., Kanai, Y., Cowan, N. J., & Hirokawa, N. (1992) · Nature · doi:10.1038/360674a0 · Cited by 601
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Cited by
Sorted by citations
Roles of tau protein in health and disease
Guo, T., Noble, W., & Hanger, D. P. (2017) · Acta Neuropathologica · doi:10.1007/s00401-017-1707-9 · Cited by 1,003
Differential Regulation of Dynein and Kinesin Motor Proteins by Tau
Dixit, R., Ross, J. L., Goldman, Y. E., & Holzbaur, E. L. F. (2008) · Science · doi:10.1126/science.1152993 · Cited by 1,012
Biochemistry and Cell Biology of Tau Protein in Neurofibrillary DegenerationPreprint
Mandelkow, E., & Mandelkow, E. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a006247 · Cited by 897
“Another indirect function is the inhibition of MT-dependent transport by motor proteins, which is based on the competition between motors (kinesin, dynein) and MAPs for binding sites on the MTsurface (Seitz et al. 2002; Stamer et al. 2002; Dixit et al. 2008).”
BackgroundSelf-citationAxonal transport deficits and neurodegenerative diseases
Millecamps, S., & Julien, J.-P. (2013) · Nature Reviews Neuroscience · doi:10.1038/nrn3380 · Cited by 755
The MAP1 family of microtubule-associated proteins.
Dehmelt (2004) · Genome biology · doi:10.1186/gb-2004-6-1-204 · Cited by 972
Metadata
- Authors
- Seitz, A., Kojima, H., Oiwa, K., Mandelkow, E.-M., Song, Y.-H., & Mandelkow, E.
- Affiliations
- Max Planck Unit for Structural Molecular Biology (DE)
- Corresponding author
- Seitz, Arne
- Venue
- The EMBO Journal
- Published
- 2002-09-16
- Volume
- 21
- Issue
- 18
- Pages
- 4896-4905
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC126299
- OpenAlex
- W1968991344
- Cited by
- 257
- References
- 49
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 12 · 2018: 7 · 2017: 15 · 2016: 11 · 2015: 12 · 2014: 18 · 2013: 10 · 2012: 12
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Desy publication database (The Deutsches Elektronen-Synchrotron) (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- kinesin, tau protein, MAP2c, microtubule-associated proteins, microtubule stabilization, single-molecule tracking, total internal reflection fluorescence microscopy, run length, processivity, motor speed, neuronal transport
- MeSH terms
- Adenosine Triphosphatases, Animals, Biological Transport, Humans, Isoelectric Point, Microscopy, Fluorescence, Microtubule-Associated Proteins, Microtubules, Models, Biological, Protein Binding, Recombinant Proteins, Kinesins, tau Proteins, Genes, Reporter, Protein Isoforms, Molecular Motor Proteins, Rats
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