The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
Spontaneous differentiation of germ cells from human embryonic stem cells in vitro
Abstract
Little is known of molecular requirements for specification of human germ cells. However, it is likely that they are specified through the action of sequentially expressed genes just as in model organisms. We sought to determine whether human embryonic stem (ES) cell lines, like those of mice, might be capable of forming germ cells in vitro. We compared transcriptional profiles of three pluripotent human ES cells to those of isolated inner cell mass (ICM) cells. We found that ICM cells expressed NANOS1, STELLAR and OCT4, whereas undifferentiated human ES cells expressed these genes along with the germ cell-specific gene, DAZL. Upon ES cell differentiation into embryoid bodies (EBs), we observed a shift in expression from RNA and protein markers of immature germ cells to those indicative of mature germ cells, including expression of VASA, BOL, SCP1, SCP3, GDF9 and TEKT1. Although ability to test the function of these putative VASA positive germ cells is limited, these results demonstrate that differentiation of human ES cells into EBs in vitro results in formation of cells that express markers specific to gonocytes.
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Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,926
Pfaffl, M. W. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.9.e36 · Cited by 7,376
Niwa, H., Miyazaki, J.-I., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,596
In vitro differentiation of transplantable neural precursors from human embryonic stem cells
Zhang, S.-C., Wernig, M., Duncan, I. D., Brüstle, O., & Thomson, J. A. (2001) · Nature Biotechnology · doi:10.1038/nbt1201-1129 · Cited by 1,912
Kehat, I., Kenyagin‐Karsenti, D., Snir, M., Segev, H., Amit, M., Gepstein, A., Livne, E., Binah, O., Itskovitz‐Eldor, J., & Gepstein, L. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12131 · Cited by 1,814
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
Ramalho-Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R. C., & Melton, D. A. (2002) · Science · doi:10.1126/science.1072530 · Cited by 1,709
Growth differentiation factor-9 is required during early ovarian folliculogenesis
Dong, J., Albertini, D. F., Nishimori, K., Kumar, T. R., Lu, N., & Matzuk, M. M. (1996) · Nature · doi:10.1038/383531a0 · Cited by 1,646
A Stem Cell Molecular Signature
Ivanova, N. B., Dimos, J. T., Schaniel, C., Hackney, J. A., Moore, K. A., & Lemischka, I. R. (2002) · Science · doi:10.1126/science.1073823 · Cited by 1,542
Embryonic Stem Cells Express Neuronal Properties in Vitro
Bain, G., Kitchens, D., Yao, M., Huettner, J. E., & Gottlieb, D. I. (1995) · Developmental Biology · doi:10.1006/dbio.1995.1085 · Cited by 1,168
BMP4 initiates human embryonic stem cell differentiation to trophoblast
Xu, R.-H., Chen, X., Li, D. S., Li, R., Addicks, G. C., Glennon, C., Zwaka, T. P., & Thomson, J. A. (2002) · Nature Biotechnology · doi:10.1038/nbt761 · Cited by 1,128
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In vitro spatially organizing the differentiation in individual multicellular stem cell aggregates
Qi, H., Huang, G., Han, Y. L., Lin, W., Li, X., Wang, S., Lu, T. J., & Xu, F. (2016) · Critical Reviews in Biotechnology · doi:10.3109/07388551.2014.922917 · Cited by 26
What Can Stem Cell Models Tell Us About Human Germ Cell Biology?
Irie, N., Sybirna, A., & Surani, M. A. (2018) · Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2018.02.010 · Cited by 22
Ganjibakhsh, M., Mehraein, F., Koruji, M., Aflatoonian, R., & Farzaneh, P. (2019) · Experimental Cell Research · doi:10.1016/j.yexcr.2019.111544 · Cited by 18
Metadata
- Authors
- Clark, A. T.
- Venue
- Human Molecular Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-01-28
- Volume
- 13
- Issue
- 7
- Pages
- 727-739
- ISSN
- 0964-6906 (ISSN-L)
- 1460-2083
- OpenAlex
- W2168684990
- Cited by
- 503
- References
- 60
- FWCI
- 10.06
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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