Tissue integrity is essential for ectopic implantation of human endometrium in the chicken chorioallantoic membrane
Abstract
BACKGROUND: Not all women with patent tubes develop clinically manifest endometriosis. Quality and quantity of endometrium in retrograde menstruation may be the determining factor in the development of the disease. We hypothesize that retrograde shedding of endometrial fragments with preserved integrity facilitates implantation of endometrium in ectopic locations, resulting in endometriotic lesion development. We evaluate the impact of tissue integrity on the success of endometriosis-like lesion development in the chicken embryo chorioallantoic membrane (CAM) model. METHODS: Menstrual and non-menstrual (cyclic) endometrium were collected by biopsy, and either minced or enzymatically dispersed. Spontaneously shed menstrual effluent was collected by a menstrual cup, and cells and tissue were isolated. We evaluated whether infiltration or lesion formation in the CAM occurred after transplantation of endometrium onto the CAM. RESULTS: Transplantation of biopsied menstrual and cyclic endometrium fragments, and of endometrium fragments >1 mm(3) isolated from menstrual effluent, resulted in lesion formation. Transplantation of endometrial cells isolated from menstrual effluent did not lead to lesion formation. After transplantation of digested biopsied cyclic endometrium, infiltration in the CAM but no lesions were observed. CONCLUSION: In the CAM assay, integrity of tissue architecture determines success of implantation of human endometrium in ectopic locations.
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For development on the chicken allantoic membrane, tissue integrity is important.
“For development on the chicken allantoic membrane, tissue integrity is important (115).”
From Pathogenesis of deep endometriosis (2017) · cites this paper as [115]
These experiments emphasize the importance of tissue integrity and of the special relationship of endometrium and junctional zone for growth and development.
“These experiments, however, emphasize the importance of tissue integrity-as for the chicken allantoic membrane experiments (115)-and of the special relationship of endometrium and junctional zone for growth and development.”
From Pathogenesis of deep endometriosis (2017) · cites this paper as [115]
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Cites
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Pathogenesis and pathophysiology of endometriosis
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- Authors
- Nap, A. W., Groothuis, P. G., Demir, A. Y., Maas, J. W. M., Dunselman, G. A. J., de Goeij, A. F. P. M., & Evers, J. L. H.
- Affiliations
- Maastricht University Medical Centre (NL)
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-01-01
- Volume
- 18
- Issue
- 1
- Pages
- 30-34
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2169469417
- Cited by
- 55
- References
- 18
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Menstrual Health and Disorders
- Keywords
- endometriosis, tissue integrity, chorioallantoic membrane model, retrograde menstruation, endometrium
- MeSH terms
- Allantois, Animals, Cell Division, Chick Embryo, Chorion, Endometriosis, Endometrium, Female, Humans, Menstruation, Transplantation, Heterologous, Transplantation, Heterotopic
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