The DOI matches its Crossref record; 73 of the 73 references it cites were found in a registry or an index.
Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility
Abstract
BACKGROUND: The aim of this study was to examine the role of apoptosis and reactive oxygen species (ROS) in inducing DNA damage in ejaculated spermatozoa. METHODS: We examined ejaculated spermatozoa from 31 patients examined for infertility and 19 healthy donors for apoptosis, production of ROS and DNA damage using annexin V binding, chemiluminescence assay and sperm chromatin structure assay. RESULTS: The percentage of spermatozoa that underwent apoptosis in the whole ejaculate and mature fraction was higher in the patients than in the donors (P<0.001 and P=0.009, respectively). Levels of ROS in the whole ejaculate and immature fraction were higher in the patients than in the donors (P=0.002 and P=0.009). Apoptosis was significantly correlated with ROS within patients in the whole ejaculate [r (95% confidence interval)=0.53 (0.19-0.86)] and in the mature [0.71 (0.39-1.00)] and immature spermatozoa [0.75 (0.45-1.00)]. Only apoptosis and the DNA fragmentation index (DFI) were significantly correlated within patients in the whole ejaculate [0.57 (0.18-0.97)]. CONCLUSIONS: DNA damage may be induced by oxidative assault. Apoptosis may not contribute significantly to the DNA damage.
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Cites
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“Measurement of mature sperm nuclear DNA damage by SCSA DNA damage was measured in separate aliquots after a simple wash and density gradient centrifugation (fractions 1 and 2) by sperm chromatin structure assay (SCSA; Evenson et al., 1999, 2002).”
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Cited by
Oxidative stress and male infertility—a clinical perspective
Tremellen, K. (2008) · Human Reproduction Update · doi:10.1093/humupd/dmn004 · Cited by 1,507
Reduction of the Incidence of Sperm DNA Fragmentation by Oral Antioxidant Treatment
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Sharma, R., Agarwal, A., Rohra, V. K., Assidi, M., Abu-Elmagd, M., & Turki, R. F. (2015) · Reproductive Biology and Endocrinology · doi:10.1186/s12958-015-0028-x · Cited by 451
Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect
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Sperm chromatin structure and male fertility: biological and clinical aspects
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Metadata
- Authors
- Moustafa, M. H.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-01-01
- Volume
- 19
- Issue
- 1
- Pages
- 129-138
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2134414426
- Cited by
- 419
- References
- 73
- FWCI
- 12.85
- Type
- journal article
- Language
- en
- Open access
- closed
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