The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Estrogen receptors α and β (ERα and ERβ) and androgen receptor (AR) in human sperm: localization of ERβ and AR in mitochondria of the midpiece
Abstract
BACKGROUND: The central role of estrogens and androgens in the male reproductive system has focused attention on the presence and distribution of their cognate receptors [estrogen receptor (ER) alpha, ERbeta and androgen receptor (AR)] in male reproductive tissues and cells. Since the presence of steroid hormone receptors in mitochondria of mammalian cells has been well documented, we investigated the possibility of mitochondrial localization of sex steroid hormone receptors in sperm. METHODS AND RESULTS: Applying immunofluorescence labelling and confocal laser scanning microscopy we show that the estrogen receptor beta and the AR of human sperm are specifically enriched in the midpiece, at the site of the mitochondria, which were visualized by labelling with the vital dye CMX. Nuclear and mitochondrial localization of AR was also detected in LnCap human prostate cancer cells. Differentially, most of the ERalpha immunostaining is in the form of a compact zone at a region corresponding to the equatorial segment of the upper post-acrosomal region of the sperm head. Immunoblotting experiments using sperm extracts revealed the presence of a 66 and a 45 kDa protein reacting with the ERalpha antibody, one 64 kDa protein reacting with the ERbeta antibody and a 110 and a 90 kDa protein reacting with the antibody against AR. CONCLUSIONS: Our findings suggest that the differential localization of AR and ER isoforms in human sperm reveals distinct roles of these receptors in the physiology of sperm cells and, perhaps, also in the process of fertilization.
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Citations
Cites
Estrogen promotes apoptosis of murine osteoclasts mediated by TGF–β
Hughes, D. E., Dai, A., Tiffee, J. C., Li, H. H., Mundy, G. R., & Boyce, B. F. (1996) · Nature Medicine · doi:10.1038/nm1096-1132 · Cited by 813 · cited 1×
“The central role of mitochondria in energy production and cell apoptosis and the known effects of steroid and thyroid hormones on these processes (Hughes et al., 1996; Scheller et al., 2003) led to the hypothesis (Sekeris, 1990) that the hormone receptors could be involved in these important regulatory processes by ac…”
BackgroundMitochondrial localization of ERα and ERβ in human MCF7 cells
Chen, J. Q., Delannoy, M., Cooke, C., & Yager, J. D. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00508.2003 · Cited by 272
Aquila, S., Sisci, D., Gentile, M., Middea, E., Catalano, S., Carpino, A., Rago, V., & Andò, S. (2004) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-031681 · Cited by 184
Mitochondrial genes as sites of primary action of steroid hormones
Demonacos, C. V., Karayanni, N., Hatzoglou, E., Tsiriyiotis, C., Spandidos, D. A., & Sekeris, C. E. (1996) · Steroids · doi:10.1016/0039-128x(96)00019-0 · Cited by 177
Self-citationDemonacos, C., Djordjevic-Markovic, R., Tsawdaroglou, N., & Sekeris, C. E. (1995) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(95)00159-w · Cited by 131
Self-citationGlucocorticoid and thyroid hormone receptors in mitochondria of animal cells
Scheller, K., Seibel, P., & Sekerist, C. E. (2003) · International review of cytology · doi:10.1016/s0074-7696(02)22011-2 · Cited by 73 · cited 1×
“The central role of mitochondria in energy production and cell apoptosis and the known effects of steroid and thyroid hormones on these processes (Hughes et al., 1996; Scheller et al., 2003) led to the hypothesis (Sekeris, 1990) that the hormone receptors could be involved in these important regulatory processes by ac…”
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Cited by
Chen, J.-Q., Cammarata, P. R., Baines, C. P., & Yager, J. D. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2009.06.001 · Cited by 264
Muscle metabolism and atrophy: let’s talk about sex
Rosa-Caldwell, M. E., & Greene, N. P. (2019) · Biology of Sex Differences · doi:10.1186/s13293-019-0257-3 · Cited by 202
Chen, J.-Q., Brown, T. R., & Russo, J. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2009.03.009 · Cited by 190
Sex differences in inflammation, redox biology, mitochondria and autoimmunity
Di Florio, D. N., Sin, J., Coronado, M. J., Atwal, P. S., & Fairweather, D. (2020) · Redox Biology · doi:10.1016/j.redox.2020.101482 · Cited by 185
Classical androgen receptors in non-classical sites in the brain
Sarkey, S., Azcoitia, I., Garcia-Segura, L. M., Garcia-Ovejero, D., & DonCarlos, L. L. (2008) · Hormones and Behavior · doi:10.1016/j.yhbeh.2008.02.015 · Cited by 146
Metadata
- Authors
- Solakidi, S., Psarra, A.-M. G., Nikolaropoulos, S., & Sekeris, C. E.
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-08-25
- Volume
- 20
- Issue
- 12
- Pages
- 3481-3487
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2100534001
- Cited by
- 206
- References
- 6
- FWCI
- 11.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Solakidi, S., Psarra, A.-M. G., Nikolaropoulos, S., & Sekeris, C. E. (2005). Estrogen receptors α and β (ERα and ERβ) and androgen receptor (AR) in human sperm: localization of ERβ and AR in mitochondria of the midpiece. Human Reproduction. https://doi.org/10.1093/humrep/dei267
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